FastQCFastQC Report
Fri 10 Feb 2017
ERR1632506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814699
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11700.14361132148192154No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8800.10801535290947946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7800.027.2756421
TAGTATG358.867169E-426.4285726
TAATACG4900.022.2755127
AATACGG5250.020.79047828
ATACGGC1251.8189894E-1120.7229
CGTATGC555.1413267E-420.18181831
AGACAGT1202.382876E-1020.0416666
GTTCAAA656.898975E-519.9230771
CTTAGGA1700.019.5882362
GTAACGT2550.019.58823626
TGTATAT951.6746162E-719.4736842
GTAATAC5800.019.45689626
GTATCAA11050.019.4208152
ATACGGA4350.019.13793229
GCGGTAA5900.019.1271223
GGTAATA6400.018.78906225
CGGTAAT6050.018.65289324
CCTAGTA500.007033164618.524
GACCGTT701.2186442E-418.57
TAAACCA1002.8723662E-718.55
Produced by FastQC (version 0.11.5)