FastQCFastQC Report
Fri 10 Feb 2017
ERR1632505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92121
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2330.2529282139794401No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1590.1725990816426222No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT1360.14763191888928692RNA PCR Primer, Index 41 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1180.12809240021276366No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1090.11832264087450202No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA990.10746735272087798No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA980.10638182390551557No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA970.10529629509015317No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC930.10095417982870356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCCG200.001833160137.09
CGCGGTA705.456968E-1231.71428522
GGATACC705.456968E-1231.7142851
CGCCCTA705.456968E-1231.71428516
TCAATGA303.574055E-430.83333232
TAATACG751.4551915E-1129.627
TACCACG751.4551915E-1129.64
AATGATA250.00547045429.64
ACGTGTC751.4551915E-1129.68
TACTCAT751.4551915E-1129.621
TCCCGCC751.4551915E-1129.613
CCGCGGT751.4551915E-1129.621
TCGAGCT751.4551915E-1129.627
CACGTGT751.4551915E-1129.67
CGGTAAT751.4551915E-1129.624
AATACGG751.4551915E-1129.628
GGTATCA1000.027.7500021
AGCCGCG803.092282E-1127.7519
GCGGTAA803.092282E-1127.7523
CCCTACT803.092282E-1127.7518
Produced by FastQC (version 0.11.5)