##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632505.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92121
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.99497400158487	34.0	33.0	34.0	31.0	34.0
2	33.14097762725112	34.0	33.0	34.0	31.0	34.0
3	33.220926824502556	34.0	33.0	34.0	31.0	34.0
4	36.51906731364184	37.0	37.0	37.0	35.0	37.0
5	36.47932610371142	37.0	37.0	37.0	35.0	37.0
6	36.523029493817916	37.0	37.0	37.0	35.0	37.0
7	36.520749883305655	37.0	37.0	37.0	35.0	37.0
8	36.484547497313315	37.0	37.0	37.0	35.0	37.0
9	38.27745030991848	39.0	39.0	39.0	37.0	39.0
10	38.292354620553404	39.0	39.0	39.0	37.0	39.0
11	38.37024131305566	39.0	39.0	39.0	37.0	39.0
12	38.328426743087896	39.0	39.0	39.0	37.0	39.0
13	38.36748406986464	39.0	39.0	39.0	37.0	39.0
14	39.90352905417874	41.0	40.0	41.0	38.0	41.0
15	39.899968519664355	41.0	40.0	41.0	38.0	41.0
16	39.86517732113199	41.0	40.0	41.0	38.0	41.0
17	39.857502632407375	41.0	40.0	41.0	38.0	41.0
18	39.85499506084389	41.0	40.0	41.0	38.0	41.0
19	39.85664506464324	41.0	40.0	41.0	38.0	41.0
20	39.84365128472335	41.0	40.0	41.0	38.0	41.0
21	39.79993703932871	41.0	40.0	41.0	38.0	41.0
22	39.75560404250931	41.0	40.0	41.0	38.0	41.0
23	39.70687465398769	41.0	40.0	41.0	38.0	41.0
24	39.69536804854485	41.0	40.0	41.0	37.0	41.0
25	39.64974327243517	41.0	40.0	41.0	37.0	41.0
26	39.54938613345491	41.0	40.0	41.0	37.0	41.0
27	39.46874219776164	41.0	39.0	41.0	37.0	41.0
28	39.42285689473627	41.0	39.0	41.0	37.0	41.0
29	39.365410709827295	41.0	39.0	41.0	36.0	41.0
30	39.338576437511534	41.0	39.0	41.0	36.0	41.0
31	39.2691351591928	41.0	39.0	41.0	36.0	41.0
32	39.214315954016996	41.0	39.0	41.0	36.0	41.0
33	39.144635859358885	41.0	39.0	41.0	35.0	41.0
34	39.0849209192258	41.0	39.0	41.0	35.0	41.0
35	39.00895561272674	40.0	39.0	41.0	35.0	41.0
36	38.9595531963396	40.0	39.0	41.0	35.0	41.0
37	38.89433462511262	40.0	39.0	41.0	35.0	41.0
38	38.81573148359223	40.0	38.0	41.0	35.0	41.0
39	38.72447107608471	40.0	38.0	41.0	35.0	41.0
40	38.66080481106371	40.0	38.0	41.0	35.0	41.0
41	38.605399420327615	40.0	38.0	41.0	35.0	41.0
42	38.53351570217431	40.0	38.0	41.0	35.0	41.0
43	37.77545836454229	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	3.0
21	12.0
22	26.0
23	33.0
24	50.0
25	109.0
26	104.0
27	163.0
28	212.0
29	311.0
30	441.0
31	551.0
32	837.0
33	999.0
34	1564.0
35	2401.0
36	3961.0
37	7450.0
38	19255.0
39	53636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99973947308432	15.958359114642699	11.817066684035128	28.224834728237862
2	21.857122697322	18.78181956340031	30.712866773048493	28.648190966229198
3	21.5944247240043	19.145471716546716	28.056577761856687	31.203525797592295
4	16.350235016988528	14.802270926281738	32.341159996092095	36.50633406063764
5	18.066456074076488	32.335732352015285	32.392179850414124	17.2056317234941
6	37.30311221111364	31.94819856493091	14.30618425766112	16.44250496629433
7	31.99379077517613	26.528153189826426	18.94899100096612	22.52906503403133
8	28.880494132716755	29.926943910726113	19.196491570868748	21.996070385688387
9	28.180328046808	12.343548159485893	16.888657309408277	42.58746648429783
10	19.465702717078624	23.025151702651947	29.575232574548693	27.933913005720733
11	41.066640613975096	18.488726783252464	17.878659589018792	22.565973013753652
12	24.210549169027693	22.423768738941174	26.229632765601764	27.13604932642937
13	35.84633254089729	16.694347651458408	19.688236124227917	27.771083683416375
14	24.924827129536155	18.47787149509884	21.79850414129243	34.79879723407257
15	29.90848992086495	23.2487706386166	19.172609936930776	27.67012950358767
16	27.184898123120675	23.0121253568676	21.15152896733644	28.651447552675286
17	27.92305771756711	22.721203634350474	21.08965382486078	28.266084823221632
18	27.64190575438825	20.896429695726273	22.4085713355261	29.053093214359375
19	29.06937614658981	21.49998371706777	21.918997839797658	27.511642296544764
20	30.169016836551926	20.24945452177028	21.552089100205166	28.029439541472627
21	28.435427318418167	21.20254882165847	21.40554271013124	28.956481149792126
22	29.288652967293018	22.190380043638257	20.336296826999273	28.18467016206945
23	29.127994702619382	21.515181120482843	21.241627859011516	28.11519631788626
24	29.125823644988657	20.960475895832655	20.964818011094106	28.948882448084586
25	29.032468166867492	21.406628238946602	21.47827314076052	28.082630453425384
26	28.940198217561687	21.623734002019084	21.401200594869792	28.034867185549437
27	28.15210429760858	21.475016554314436	22.625677098598583	27.747202049478403
28	27.518155469436934	22.151301006285212	21.4153124694695	28.91523105480835
29	28.009900022796103	22.586598061245535	21.380575547377905	28.022926368580453
30	27.736346761324782	21.661727510556766	22.522551861139153	28.079373866979303
31	28.305163860574677	21.582483907035314	21.74097111407822	28.371381118311785
32	26.75719976986789	21.533635110344004	22.173011582592462	29.53615353719565
33	27.530096286405925	21.05817348921527	23.1130795366963	28.298650687682503
34	27.627793879788538	21.168897428382234	22.90357247533136	28.299736216497866
35	26.753943183421804	22.103537738409266	23.468047459319806	27.67447161884912
36	27.315161580964165	21.150443438521073	23.818673266681863	27.71572171383289
37	27.15450331629053	20.52952095613378	24.066173836584493	28.249801890991193
38	26.12542199932697	20.179980677587086	25.34709783871213	28.347499484373813
39	25.615223456106644	19.737084920919227	26.012527002529286	28.635164620444847
40	25.50124293049359	20.20820442678651	27.056805722907917	27.233746919811985
41	24.053147490800143	19.548202907046168	27.84598517167638	28.55266443047731
42	22.713604932642937	20.51215249508798	28.73069115619674	28.043551416072336
43	22.473703064447843	20.405770671182466	28.264999294406266	28.855526969963417
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	0.5
4	0.0
5	0.0
6	2.0
7	4.0
8	3.0
9	2.0
10	4.0
11	6.0
12	6.0
13	15.0
14	24.0
15	30.5
16	37.0
17	41.0
18	45.0
19	45.0
20	64.0
21	83.0
22	82.5
23	82.0
24	91.5
25	101.0
26	101.0
27	148.5
28	196.0
29	258.5
30	321.0
31	424.0
32	527.0
33	527.0
34	717.5
35	908.0
36	1068.5
37	1229.0
38	1623.0
39	2017.0
40	2017.0
41	2374.5
42	2732.0
43	3204.5
44	3677.0
45	4503.5
46	5330.0
47	5330.0
48	6067.5
49	6805.0
50	7660.5
51	8516.0
52	8905.5
53	9295.0
54	9295.0
55	9165.0
56	9035.0
57	8922.5
58	8810.0
59	8488.0
60	8166.0
61	8166.0
62	7637.5
63	7109.0
64	6160.0
65	5211.0
66	4580.0
67	3949.0
68	3949.0
69	3378.5
70	2808.0
71	2436.5
72	2065.0
73	1640.0
74	1215.0
75	1215.0
76	963.0
77	711.0
78	568.0
79	425.0
80	369.5
81	314.0
82	314.0
83	252.0
84	190.0
85	136.0
86	82.0
87	68.5
88	55.0
89	55.0
90	37.0
91	19.0
92	16.0
93	13.0
94	9.0
95	5.0
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	92121.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.40408810151865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.77926378071778	68.45127603912246
2	5.169658667204123	7.7962679519327835
3	1.4396153347825462	3.256586446087211
4	0.6852568993564919	2.066846864450017
5	0.41460921641737325	1.5631614941218615
6	0.30231922030433467	1.3677663073566289
7	0.17563307084347063	0.9270416083194929
8	0.15979730216086263	0.9639495880418146
9	0.11516922678260369	0.7815807470609307
>10	0.7111699753825778	9.807752846799318
>50	0.04030922937391129	2.198195851108868
>100	0.007198076673912731	0.8195742555986149
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	233	0.2529282139794401	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	159	0.1725990816426222	No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	136	0.14763191888928692	RNA PCR Primer, Index 41 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	118	0.12809240021276366	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	109	0.11832264087450202	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	99	0.10746735272087798	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	98	0.10638182390551557	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	97	0.10529629509015317	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	93	0.10095417982870356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0010855288153624038	0.0
19	0.0	0.0	0.0	0.0010855288153624038	0.0
20	0.0	0.0	0.0	0.0010855288153624038	0.0
21	0.0	0.0	0.0	0.0010855288153624038	0.0
22	0.0	0.0	0.0	0.0021710576307248076	0.0
23	0.0	0.0	0.0	0.0032565864460872116	0.0
24	0.0	0.0	0.0	0.005427644076812019	0.0
25	0.0	0.0	0.0	0.006513172892174423	0.0
26	0.0	0.0	0.0	0.010855288153624038	0.0
27	0.0	0.0	0.0	0.04559221024522096	0.0
28	0.0	0.0	0.0	0.1443753324431997	0.0
29	0.0	0.0	0.0	0.2648690309484265	0.0
30	0.0	0.0	0.0	0.4548365736368472	0.0
31	0.0	0.0	0.0	0.9378968964731169	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCCG	20	0.0018331601	37.0	9
CGCGGTA	70	5.456968E-12	31.714285	22
GGATACC	70	5.456968E-12	31.714285	1
CGCCCTA	70	5.456968E-12	31.714285	16
TCAATGA	30	3.574055E-4	30.833332	32
TAATACG	75	1.4551915E-11	29.6	27
TACCACG	75	1.4551915E-11	29.6	4
AATGATA	25	0.005470454	29.6	4
ACGTGTC	75	1.4551915E-11	29.6	8
TACTCAT	75	1.4551915E-11	29.6	21
TCCCGCC	75	1.4551915E-11	29.6	13
CCGCGGT	75	1.4551915E-11	29.6	21
TCGAGCT	75	1.4551915E-11	29.6	27
CACGTGT	75	1.4551915E-11	29.6	7
CGGTAAT	75	1.4551915E-11	29.6	24
AATACGG	75	1.4551915E-11	29.6	28
GGTATCA	100	0.0	27.750002	1
AGCCGCG	80	3.092282E-11	27.75	19
GCGGTAA	80	3.092282E-11	27.75	23
CCCTACT	80	3.092282E-11	27.75	18
>>END_MODULE