FastQCFastQC Report
Fri 10 Feb 2017
ERR1632504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17867
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1110.621257066099513No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA950.5317064980130968No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA710.3973806458834723No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA520.291039346280853No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA490.27424861476464996No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA490.27424861476464996No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT460.2574578832484469No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC460.2574578832484469No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA450.25186097274304586No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG440.2462640622376448No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA440.2462640622376448No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC430.2406671517322438No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC430.2406671517322438No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT420.23507024122684278No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT420.23507024122684278No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT400.22387642021604076No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG400.22387642021604076No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA380.2126825992052387No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT380.2126825992052387No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC360.20148877819443667No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC360.20148877819443667No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC360.20148877819443667No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC350.19589186768903566No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC350.19589186768903566No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC350.19589186768903566No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC330.1846980466782336No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC330.1846980466782336No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT330.1846980466782336No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC330.1846980466782336No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT320.1791011361728326No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC320.1791011361728326No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC320.1791011361728326No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC320.1791011361728326No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG320.1791011361728326No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT310.17350422566743157No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA310.17350422566743157No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA300.16790731516203056No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA300.16790731516203056No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG290.16231040465662952No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC280.1567134941512285No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC280.1567134941512285No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT270.1511165836458275RNA PCR Primer, Index 33 (95% over 22bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG270.1511165836458275No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT260.1455196731404265No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC260.1455196731404265No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA260.1455196731404265No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT260.1455196731404265No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA260.1455196731404265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT250.13992276263502548No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC250.13992276263502548No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC250.13992276263502548No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG250.13992276263502548No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG240.13432585212962445No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC240.13432585212962445No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG240.13432585212962445No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA230.12872894162422344No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA230.12872894162422344No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA230.12872894162422344No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC230.12872894162422344No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA230.12872894162422344No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA220.1231320311188224No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT220.1231320311188224No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG220.1231320311188224No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC210.11753512061342139No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA210.11753512061342139No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG210.11753512061342139No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG210.11753512061342139No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT200.11193821010802038No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA200.11193821010802038No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG200.11193821010802038No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC190.10634129960261934No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG190.10634129960261934No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG190.10634129960261934No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA190.10634129960261934No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG190.10634129960261934No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG190.10634129960261934No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG180.10074438909721833No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC180.10074438909721833No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC180.10074438909721833No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA180.10074438909721833No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT180.10074438909721833No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG180.10074438909721833No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT180.10074438909721833No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG180.10074438909721833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT180.10074438909721833No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA180.10074438909721833No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC180.10074438909721833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC608.7606494E-418.51
ATCAACG608.7606494E-418.52
TCAACGC650.001499136417.0769233
CAACGCA650.001499136417.0769234
AACGCAG700.002460974715.8571435
ACGCAGA700.002460974715.8571436
CGCAGAG700.002460974715.8571437
CAGAGTA750.003897859514.89
AGTACGG750.003897859514.812
AGAGTAC750.003897859514.810
GAGTACG750.003897859514.811
GCAGAGT800.00598421613.8758
CTGTCTC1551.6093181E-613.12903237
GTACGGG850.00893908113.05882413
Produced by FastQC (version 0.11.5)