FastQCFastQC Report
Fri 10 Feb 2017
ERR1632503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14410
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA940.6523247744621791No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT800.5551700208188758TruSeq Adapter, Index 5 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA570.395558639833449No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA520.3608605135322693No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA500.3469812630117973No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA500.3469812630117973No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA450.31228313671061764No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA420.2914642609299098No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC420.2914642609299098No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT410.28452463566967384No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC410.28452463566967384No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC390.27064538514920194No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA380.263705759888966No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG370.25676613462873005No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA360.24982650936849407No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT340.2359472588480222No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA340.2359472588480222No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC330.22900763358778628No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC320.22206800832755033No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT300.2081887578070784No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC300.2081887578070784No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT300.2081887578070784No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT290.20124913254684246No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA290.20124913254684246No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA290.20124913254684246No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC280.19430950728660654No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA280.19430950728660654No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC280.19430950728660654No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT280.19430950728660654No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG270.18736988202637056No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG270.18736988202637056No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA260.18043025676613464No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC250.17349063150589866No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT250.17349063150589866No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG240.16655100624566274No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA240.16655100624566274No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG240.16655100624566274No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG240.16655100624566274No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC240.16655100624566274No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA230.15961138098542677No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC230.15961138098542677No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC230.15961138098542677No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG230.15961138098542677No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG230.15961138098542677No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA230.15961138098542677No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC220.15267175572519084No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA220.15267175572519084No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA220.15267175572519084No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC220.15267175572519084No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT220.15267175572519084No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC210.1457321304649549No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC210.1457321304649549No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT210.1457321304649549No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG200.13879250520471895No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT200.13879250520471895No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA200.13879250520471895No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA190.131852879944483No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC190.131852879944483No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT190.131852879944483No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT180.12491325468424704No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC180.12491325468424704No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT180.12491325468424704No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC180.12491325468424704No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC180.12491325468424704No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC170.1179736294240111No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC170.1179736294240111No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT170.1179736294240111No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA160.11103400416377517No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA160.11103400416377517No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC160.11103400416377517No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA160.11103400416377517No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG160.11103400416377517No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT150.1040943789035392No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG150.1040943789035392No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT150.1040943789035392No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA150.1040943789035392No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC150.1040943789035392No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC150.1040943789035392No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG150.1040943789035392No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT150.1040943789035392No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG150.1040943789035392No Hit
GGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGC150.1040943789035392No Hit
CTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAA150.1040943789035392No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC150.1040943789035392No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC150.1040943789035392No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTGC200.001782245937.035
GGAGGGT200.001782245937.033
GAGGGTG200.001782245937.034
CAGCAGC352.2298716E-531.71428515
CCAGCAG352.2298716E-531.71428514
TACGGAG352.2298716E-531.71428530
GCGGTAA352.2298716E-531.71428523
GCCAGCA352.2298716E-531.71428513
GCAGCCG352.2298716E-531.71428517
ATACGGA352.2298716E-531.71428529
CGCGGTA352.2298716E-531.71428522
CGTGCCA352.2298716E-531.71428510
TAATACG352.2298716E-531.71428527
AATACGG352.2298716E-531.71428528
GTAATAC352.2298716E-531.71428526
CGGTAAT352.2298716E-531.71428524
GGTAATA352.2298716E-531.71428525
CCGCGGT352.2298716E-531.71428521
GTGCCAG352.2298716E-531.71428511
CGGAGGG250.005320228629.59999832
Produced by FastQC (version 0.11.5)