FastQCFastQC Report
Fri 10 Feb 2017
ERR1632502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23287
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1130.48524928071456175TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1050.4508953493365397No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA750.3220681066689569No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA620.2662429681796711No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA600.25765448533516555No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA590.2533602439129128No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA540.231889036801649No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA530.22759479537939623No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA510.2190063125348907No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC500.21471207111263793No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT500.21471207111263793No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT480.20612358826813246No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA470.2018293468458797No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT460.19753510542362693No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC450.19324086400137414No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT440.1889466225791214No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT430.18465238115686866No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC430.18465238115686866No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC410.17606389831236313No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT410.17606389831236313No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA400.17176965689011037No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC400.17176965689011037No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG390.1674754154678576No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC380.16318117404560484No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC380.16318117404560484No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA380.16318117404560484No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG370.15888693262335207No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT360.1545926912010993No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA350.15029844977884654No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC350.15029844977884654No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC340.1460042083565938RNA PCR Primer, Index 10 (95% over 22bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC340.1460042083565938No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC330.14170996693434107No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA330.14170996693434107No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA320.1374157255120883No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC310.13312148408983554No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC310.13312148408983554No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC310.13312148408983554No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT300.12882724266758278No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA300.12882724266758278No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG300.12882724266758278No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG290.12453300124533001No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA290.12453300124533001No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA290.12453300124533001No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG280.12023875982307725No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA270.1159445184008245No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG270.1159445184008245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT260.11165027697857173No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC260.11165027697857173No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT260.11165027697857173No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC260.11165027697857173No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA260.11165027697857173No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC250.10735603555631897No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA250.10735603555631897No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC240.10306179413406623No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC240.10306179413406623No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC240.10306179413406623No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGA307.966986E-637.029
GGTATCA452.391971E-937.01
AATACGG401.5127298E-632.37528
TACGGAG352.2899554E-531.71428530
CGGAGGG303.493092E-430.83333232
CAGCACC250.005387664329.64
CGTGCCA502.532306E-729.610
GGAGGGT250.005387664329.633
GCGGTAA453.7909595E-628.77777723
AACTCCG453.7909595E-628.7777775
GCAGCCG453.7909595E-628.77777717
TAATACG453.7909595E-628.77777727
AGCCGCG453.7909595E-628.77777719
GTAATAC453.7909595E-628.77777726
CTCCGTG453.7909595E-628.7777777
CGGTAAT453.7909595E-628.77777724
GGTAATA453.7909595E-628.77777725
GTGCCAG453.7909595E-628.77777711
ACGGAGG358.6096936E-426.4285731
CCAGCAG508.601601E-625.90000214
Produced by FastQC (version 0.11.5)