##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632502.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23287
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60578863743719	34.0	31.0	34.0	31.0	34.0
2	32.77635590672907	34.0	31.0	34.0	31.0	34.0
3	32.84394726671533	34.0	31.0	34.0	31.0	34.0
4	36.316915016962255	37.0	37.0	37.0	35.0	37.0
5	36.17687980418259	37.0	35.0	37.0	35.0	37.0
6	36.22991368574741	37.0	37.0	37.0	35.0	37.0
7	36.23429381199811	37.0	37.0	37.0	35.0	37.0
8	36.202602310301884	37.0	36.0	37.0	35.0	37.0
9	37.918710009876754	39.0	38.0	39.0	35.0	39.0
10	37.87022802421952	39.0	38.0	39.0	35.0	39.0
11	37.96345600549663	39.0	38.0	39.0	35.0	39.0
12	37.881307167088934	39.0	38.0	39.0	35.0	39.0
13	37.93154979172929	39.0	38.0	39.0	35.0	39.0
14	39.31107484862799	40.0	39.0	41.0	37.0	41.0
15	39.32704942671877	40.0	39.0	41.0	37.0	41.0
16	39.25563619186671	40.0	39.0	41.0	36.0	41.0
17	39.227981277107396	40.0	39.0	41.0	36.0	41.0
18	39.23772920513591	40.0	39.0	41.0	36.0	41.0
19	39.224846480869154	40.0	39.0	41.0	36.0	41.0
20	39.22123931807446	40.0	39.0	41.0	36.0	41.0
21	39.186799501867995	40.0	39.0	41.0	36.0	41.0
22	39.12929960922403	40.0	39.0	41.0	36.0	41.0
23	39.06136470992399	40.0	39.0	41.0	36.0	41.0
24	39.04616309528922	40.0	39.0	41.0	36.0	41.0
25	39.03358096792201	40.0	38.0	41.0	36.0	41.0
26	38.898999441748614	40.0	38.0	41.0	35.0	41.0
27	38.74706918022931	40.0	38.0	41.0	35.0	41.0
28	38.727788036243396	40.0	38.0	41.0	35.0	41.0
29	38.69566711040495	40.0	38.0	41.0	35.0	41.0
30	38.62910636836003	40.0	38.0	41.0	35.0	41.0
31	38.54292094301542	40.0	38.0	41.0	35.0	41.0
32	38.4727530381758	40.0	38.0	41.0	34.0	41.0
33	38.35083952419805	40.0	38.0	41.0	34.0	41.0
34	38.35556318976253	40.0	38.0	41.0	34.0	41.0
35	38.244557048997294	40.0	38.0	41.0	34.0	41.0
36	38.206638897238804	40.0	38.0	41.0	34.0	41.0
37	38.10765663245588	40.0	38.0	41.0	34.0	41.0
38	38.04294241422253	40.0	37.0	41.0	34.0	41.0
39	37.85661527891098	40.0	37.0	41.0	33.0	41.0
40	37.77777300639842	40.0	37.0	41.0	33.0	41.0
41	37.7012925666681	40.0	37.0	41.0	33.0	41.0
42	37.64374973160991	40.0	36.0	41.0	33.0	41.0
43	36.76995748700992	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	7.0
22	11.0
23	22.0
24	22.0
25	27.0
26	49.0
27	72.0
28	107.0
29	131.0
30	167.0
31	198.0
32	294.0
33	406.0
34	662.0
35	975.0
36	1548.0
37	3015.0
38	7237.0
39	8332.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.5688152187916	16.51135826856186	13.264911753338774	27.65491475930777
2	22.746596813672866	19.710568128140164	29.995276334435523	27.547558723751447
3	22.13252029029072	18.77012925666681	29.802035470434145	29.29531498260832
4	15.92304719371323	16.378236784472023	34.242281101043496	33.456434920771244
5	17.842573109460215	31.665736247691846	32.47734787649762	18.014342766350325
6	32.773650534633056	33.80856271739597	15.639627259844548	17.77815948812642
7	29.235195602696784	27.99845407308799	19.263967020225877	23.502383303989347
8	27.216902134237987	29.1063683600292	20.990252071971486	22.686477433761326
9	28.556705457980847	12.393180744621462	17.164082964744278	41.88603083265341
10	19.929574440675054	22.463176879804184	27.916863486065186	29.690385193455576
11	40.59346416455533	18.868896809378626	17.949929145016533	22.587709881049513
12	22.50611929402671	24.09498862026023	26.04886846738524	27.350023618327825
13	37.2868982694207	17.237085068922575	18.946193154979174	26.52982350667755
14	24.77347876497617	20.410529479967362	22.433117189848414	32.382874565208056
15	30.884184308841846	23.21896337012067	19.00631253489071	26.89053978614678
16	25.499205565336887	23.210374887276163	22.609181088160778	28.681238459226176
17	27.440202688195132	23.41220423412204	20.638124275346758	28.50946880233607
18	27.195430927126722	19.852278095074503	22.823893159273414	30.12839781852536
19	29.059131704384424	21.69880190664319	22.360115085670117	26.88195130330227
20	31.837505904581953	19.727745093829174	21.217846867350882	27.216902134237987
21	28.21746038562288	21.363851075707476	20.844247863614893	29.574440675054753
22	29.939451195946237	22.179756945935498	20.131403787520934	27.74938807059733
23	29.583029157899254	20.88719027783742	21.131962038905826	28.397818525357494
24	28.011336797354748	20.346115858633574	22.205522394469018	29.43702494954266
25	28.874479323227554	21.32090866148495	22.729419847983852	27.075192167303648
26	29.424142225275908	21.96075063340061	21.74603856228797	26.869068579035517
27	27.264138789882768	22.016575771889897	24.00480955039292	26.714475887834414
28	26.555588955211064	22.454588396959675	21.411087731352257	29.578734916477007
29	26.980718856014086	23.184609438742644	21.68162494095418	28.153046764289087
30	26.66294499076738	21.282260488684674	24.262464035728087	27.792330484819853
31	29.097779877184692	21.209258384506377	21.93069094344484	27.762270794864087
32	26.418173229698972	21.162021728861596	22.622063812427534	29.797741229011894
33	26.070339674496502	21.381028041396487	24.322583415639627	28.226048868467384
34	26.6157083351226	21.346674110018466	23.352084854210503	28.68553270064843
35	26.169107227208315	23.40790999269979	24.361231588439903	26.061751191651993
36	27.255550307038263	21.34237986859621	23.897453514836602	27.504616309528924
37	26.516940782410785	21.436853179885773	23.98763258470391	28.05857345299953
38	25.8341563962726	20.706832138102804	25.112723837334133	28.34628762829046
39	25.928629707562163	19.753510542362694	26.431055953965732	27.886803796109415
40	25.340318632713533	20.814188173659122	27.414437239661616	26.431055953965732
41	23.39073302701078	20.255936788766267	27.697857173530295	28.65547301069266
42	21.531326491175335	20.94730965774896	30.386052303860524	27.135311547215185
43	20.56512217116846	21.720273113754455	28.46652638811354	29.24807832696354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	4.0
9	6.0
10	6.0
11	6.0
12	6.0
13	9.0
14	12.0
15	18.5
16	25.0
17	23.5
18	22.0
19	22.0
20	36.0
21	50.0
22	36.5
23	23.0
24	27.0
25	31.0
26	31.0
27	32.5
28	34.0
29	57.5
30	81.0
31	90.0
32	99.0
33	99.0
34	126.0
35	153.0
36	184.0
37	215.0
38	270.0
39	325.0
40	325.0
41	401.0
42	477.0
43	631.0
44	785.0
45	1235.0
46	1685.0
47	1685.0
48	1950.5
49	2216.0
50	2445.5
51	2675.0
52	2733.5
53	2792.0
54	2792.0
55	2643.5
56	2495.0
57	2418.5
58	2342.0
59	2220.5
60	2099.0
61	2099.0
62	1890.0
63	1681.0
64	1294.5
65	908.0
66	789.0
67	670.0
68	670.0
69	550.0
70	430.0
71	373.0
72	316.0
73	234.5
74	153.0
75	153.0
76	140.5
77	128.0
78	104.5
79	81.0
80	86.5
81	92.0
82	92.0
83	85.0
84	78.0
85	59.0
86	40.0
87	38.0
88	36.0
89	36.0
90	25.0
91	14.0
92	11.0
93	8.0
94	5.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.92351956026968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.09520234764211	53.54489629406965
2	7.056575445301304	8.880491261218705
3	2.531904729406947	4.779490702967321
4	1.4809254077663279	3.727401554515395
5	0.9008394185491027	2.834199338686821
6	0.49819149662185214	1.8808777429467085
7	0.4231215450760936	1.8637007772576974
8	0.27298164198457653	1.374157255120883
9	0.2047362314884324	1.159445184008245
>10	1.4741008667167133	17.24137931034483
>50	0.04777178734730089	1.7778159488126424
>100	0.013649082099228828	0.9361446300511014
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	113	0.48524928071456175	TruSeq Adapter, Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	105	0.4508953493365397	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	75	0.3220681066689569	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	62	0.2662429681796711	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	60	0.25765448533516555	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	59	0.2533602439129128	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	54	0.231889036801649	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	53	0.22759479537939623	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	51	0.2190063125348907	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	50	0.21471207111263793	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	50	0.21471207111263793	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	48	0.20612358826813246	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	47	0.2018293468458797	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	46	0.19753510542362693	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	45	0.19324086400137414	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	44	0.1889466225791214	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	43	0.18465238115686866	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	43	0.18465238115686866	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	41	0.17606389831236313	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	41	0.17606389831236313	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	40	0.17176965689011037	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	40	0.17176965689011037	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	39	0.1674754154678576	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	38	0.16318117404560484	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	38	0.16318117404560484	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	38	0.16318117404560484	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	37	0.15888693262335207	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	36	0.1545926912010993	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	35	0.15029844977884654	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	35	0.15029844977884654	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	34	0.1460042083565938	RNA PCR Primer, Index 10 (95% over 22bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	34	0.1460042083565938	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	33	0.14170996693434107	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	33	0.14170996693434107	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	32	0.1374157255120883	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	31	0.13312148408983554	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	31	0.13312148408983554	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	31	0.13312148408983554	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	30	0.12882724266758278	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	30	0.12882724266758278	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	30	0.12882724266758278	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	29	0.12453300124533001	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	29	0.12453300124533001	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	29	0.12453300124533001	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	28	0.12023875982307725	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	27	0.1159445184008245	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	27	0.1159445184008245	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.11165027697857173	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	26	0.11165027697857173	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	26	0.11165027697857173	No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC	26	0.11165027697857173	No Hit
ACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGA	26	0.11165027697857173	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	25	0.10735603555631897	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	25	0.10735603555631897	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	24	0.10306179413406623	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	24	0.10306179413406623	No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC	24	0.10306179413406623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.004294241422252759	0.0
21	0.0	0.0	0.0	0.008588482844505517	0.0
22	0.0	0.0	0.0	0.008588482844505517	0.0
23	0.0	0.0	0.0	0.012882724266758277	0.0
24	0.0	0.0	0.0	0.012882724266758277	0.0
25	0.0	0.0	0.0	0.012882724266758277	0.0
26	0.0	0.0	0.0	0.017176965689011035	0.0
27	0.0	0.0	0.0	0.04723665564478035	0.0
28	0.0	0.0	0.0	0.1159445184008245	0.0
29	0.0	0.0	0.0	0.3048911409799459	0.0
30	0.0	0.0	0.0	0.5968995576931335	0.0
31	0.0	0.0	0.0	1.0993258040967062	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGA	30	7.966986E-6	37.0	29
GGTATCA	45	2.391971E-9	37.0	1
AATACGG	40	1.5127298E-6	32.375	28
TACGGAG	35	2.2899554E-5	31.714285	30
CGGAGGG	30	3.493092E-4	30.833332	32
CAGCACC	25	0.0053876643	29.6	4
CGTGCCA	50	2.532306E-7	29.6	10
GGAGGGT	25	0.0053876643	29.6	33
GCGGTAA	45	3.7909595E-6	28.777777	23
AACTCCG	45	3.7909595E-6	28.777777	5
GCAGCCG	45	3.7909595E-6	28.777777	17
TAATACG	45	3.7909595E-6	28.777777	27
AGCCGCG	45	3.7909595E-6	28.777777	19
GTAATAC	45	3.7909595E-6	28.777777	26
CTCCGTG	45	3.7909595E-6	28.777777	7
CGGTAAT	45	3.7909595E-6	28.777777	24
GGTAATA	45	3.7909595E-6	28.777777	25
GTGCCAG	45	3.7909595E-6	28.777777	11
ACGGAGG	35	8.6096936E-4	26.42857	31
CCAGCAG	50	8.601601E-6	25.900002	14
>>END_MODULE