FastQCFastQC Report
Fri 10 Feb 2017
ERR1632500.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632500.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163498
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA6440.3938886102582294No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4730.28930017492568716No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4140.2532141065945761No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3230.19755593340591324No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3100.1896047658075328No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3080.18838150925393582No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3070.18776988097713734No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2900.17737220027156295No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2820.172479174057175No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2760.16880940439638406No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2690.1645280064587946No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2640.16146986507480213No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2580.15780009541401116No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2570.15718846713721268No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2420.1480140429852353No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG2280.13945124711005638No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2250.1376163622796609No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT2230.13639310572606392No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2170.13272333606527295No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2150.13150007951167597No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2150.13150007951167597No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2010.12293728363649709No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1970.12049077052930311No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA1930.11804425742210914No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA1910.11682100086851215No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC1900.11620937259171366No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC1890.11559774431491515No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC1870.11437448776131819No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1870.11437448776131819No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1810.11070471810052723No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT1810.11070471810052723No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1740.10642332016293776No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC1720.1052000636093408No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1710.10458843533254229No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG1670.10214192222534832No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG1660.10153029394854983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGCCG200.001837321237.011
GGTATCA3300.029.7121221
GCCCCTA250.005482730529.627
CTCACAA250.005482730529.632
ATGCCGT405.909482E-527.7534
GCCGTCT509.02895E-625.90000236
CCGCGGT2900.025.5172421
GCGGTAA2850.025.31578823
TTGGAGC451.3164397E-424.66666833
GGTAATA2950.024.45762625
CGCGGTA2950.024.45762622
CGTGCCA2950.024.45762610
CGGTAAT3000.024.0524
GTAATAC3100.023.27419326
GGCACTC400.001924146523.12520
AGCCGCG3150.022.90476219
GCAGCCG3150.022.90476217
TAATACG2750.022.87272627
CGGAGCT903.767127E-922.61111332
AATACGG2800.022.46428528
Produced by FastQC (version 0.11.5)