FastQCFastQC Report
Fri 10 Feb 2017
ERR1632499.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632499.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11607
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA770.6633927802188334No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT730.628930817610063RNA PCR Primer, Index 47 (95% over 22bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA620.5341604204359438No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA470.40492806065305414No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC390.33600413543551305No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA370.3187731541311278No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC360.31015766347893514No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT320.2756957008701646No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA320.2756957008701646No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT310.2670802102179719No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT300.25846471956577927No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT290.24984922891358666No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC290.24984922891358666No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC290.24984922891358666No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC270.23261824760920136No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG270.23261824760920136No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA270.23261824760920136No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG260.22400275695700872No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC260.22400275695700872No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC250.21538726630481606No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC250.21538726630481606No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA250.21538726630481606No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA240.20677177565262342No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG240.20677177565262342No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC230.19815628500043078No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA230.19815628500043078No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA230.19815628500043078No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC230.19815628500043078RNA PCR Primer, Index 47 (95% over 23bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA230.19815628500043078No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC220.18954079434823815No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT220.18954079434823815No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA220.18954079434823815No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC210.18092530369604548No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG210.18092530369604548No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA190.1636943223916602No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA190.1636943223916602No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG190.1636943223916602No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC180.15507883173946757No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT180.15507883173946757No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC180.15507883173946757No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC180.15507883173946757No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC170.1464633410872749No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC170.1464633410872749No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA170.1464633410872749No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA170.1464633410872749No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA170.1464633410872749No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA170.1464633410872749No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA160.1378478504350823No Hit
GAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGAGGAA160.1378478504350823No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA160.1378478504350823No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG160.1378478504350823No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT160.1378478504350823No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT160.1378478504350823No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG150.12923235978288963No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT150.12923235978288963No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC150.12923235978288963No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG150.12923235978288963No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG150.12923235978288963No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT150.12923235978288963No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC150.12923235978288963No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC150.12923235978288963No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA150.12923235978288963No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA140.12061686913069698No Hit
TGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTC140.12061686913069698No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG140.12061686913069698No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC140.12061686913069698No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT130.11200137847850436No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT130.11200137847850436No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC130.11200137847850436No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA130.11200137847850436No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC130.11200137847850436No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA130.11200137847850436No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC130.11200137847850436No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG130.11200137847850436No Hit
ACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGC120.10338588782631171No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG120.10338588782631171No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA120.10338588782631171No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACCTGTCTCTTAT120.10338588782631171No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC120.10338588782631171No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACA120.10338588782631171No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC120.10338588782631171No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT120.10338588782631171No Hit
TGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACC120.10338588782631171No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT120.10338588782631171No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA120.10338588782631171No Hit
CCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGC120.10338588782631171No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC120.10338588782631171No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT120.10338588782631171No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTTAT120.10338588782631171No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG120.10338588782631171No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG120.10338588782631171No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG120.10338588782631171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA251.15993986E-436.9999961
TCCGAGC251.15993986E-436.9999963
CTCCGAG251.15993986E-436.9999962
AGCCCAC303.3864315E-430.8333347
GTATGCC303.3864315E-430.83333432
ATCTCGT303.3864315E-430.83333427
ACATGCG303.3864315E-430.83333417
TGCGCAG303.3864315E-430.83333420
TGCCGTC303.3864315E-430.83333435
CCACGAG303.3864315E-430.83333410
TATGCCG303.3864315E-430.83333433
CCGTCTT303.3864315E-430.83333437
GAGCCCA303.3864315E-430.8333346
CACGAGA303.3864315E-430.83333411
ATGCGCA303.3864315E-430.83333419
CCCACGA303.3864315E-430.8333349
GACATGC303.3864315E-430.83333416
TCTCGTA303.3864315E-430.83333428
ACGAGAC303.3864315E-430.83333412
GATCTCG303.3864315E-430.83333426
Produced by FastQC (version 0.11.5)