FastQCFastQC Report
Fri 10 Feb 2017
ERR1632498.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632498.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA120.5263157894736842No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT120.5263157894736842TruSeq Adapter, Index 1 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA90.39473684210526316No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT80.3508771929824561No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA80.3508771929824561No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC60.2631578947368421No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA60.2631578947368421No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA60.2631578947368421No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA50.21929824561403508No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC50.21929824561403508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50.21929824561403508No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG50.21929824561403508No Hit
GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT50.21929824561403508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17543859649122806No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC40.17543859649122806No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC40.17543859649122806No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC40.17543859649122806No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA40.17543859649122806No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC40.17543859649122806No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC40.17543859649122806No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG40.17543859649122806No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC40.17543859649122806No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG40.17543859649122806No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT40.17543859649122806No Hit
GATTAACGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACG40.17543859649122806No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA40.17543859649122806No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA40.17543859649122806No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC40.17543859649122806No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT40.17543859649122806No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA40.17543859649122806No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC40.17543859649122806No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA40.17543859649122806No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA30.13157894736842105No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG30.13157894736842105No Hit
AACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTA30.13157894736842105No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC30.13157894736842105No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG30.13157894736842105No Hit
ACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCG30.13157894736842105No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC30.13157894736842105No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA30.13157894736842105No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT30.13157894736842105No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG30.13157894736842105No Hit
GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGG30.13157894736842105No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA30.13157894736842105No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC30.13157894736842105No Hit
GAGTAAAGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAG30.13157894736842105No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG30.13157894736842105No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATA30.13157894736842105No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC30.13157894736842105No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC30.13157894736842105No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT30.13157894736842105No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG30.13157894736842105No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA30.13157894736842105No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA30.13157894736842105No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG30.13157894736842105No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA30.13157894736842105No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC30.13157894736842105No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)