FastQCFastQC Report
Fri 10 Feb 2017
ERR1632496.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632496.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262165
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10880.4150058169473423No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8190.31239868022047185No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6510.24831689966242632No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6070.2315335761829382No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5400.20597715179371767No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4770.18194648408445063No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4650.17736921404459025No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4600.1754620181946484No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4450.16974043064482291No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4290.16363740392500906No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC4280.16325596475502072No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4080.15562718135525336No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3820.14570976293555585No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA3760.14342112791562567No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3590.1369366620258234No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3470.13235939198596303No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3470.13235939198596303No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3410.13007075696603285No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3380.12892643945606774No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3370.12854500028607938No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3370.12854500028607938No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3320.12663780443613756No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3310.1262563652661492No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3300.12587492609616083No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3230.12320485190624225No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3220.12282341273625388No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3150.12015333854633531No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3090.11786470352640513No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3070.1171018251864284No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3060.11672038601644004No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3030.11557606850647494No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA2990.11405031182652146No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA2900.11061735929662617No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2880.10985448095664944No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2790.10642152842675415No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2780.10604008925676578No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC2760.10527721091678904No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2730.10413289340682394No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2680.1022256975568821No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2660.10146281921690539No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2630.10031850170694029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGC355.6092176E-737.01
GGTATCA4150.033.8795171
TTTTACG303.5919694E-430.8333324
ATACACA453.992758E-628.77777937
TCCTACA556.2308027E-726.909092
CAAGACA358.851067E-426.4285724
TACGGCT1103.6379788E-1223.54545430
GTATCAA5850.023.4017092
TTTACGA400.001927234223.1250025
CTTTTTA400.001927234223.1250022
GGACGTA400.001927234223.12500227
ACTGTGC400.001927234223.1250028
TTACGAG400.001927234223.1250026
ATACGGC1054.0017767E-1122.9047629
CCTACAC652.6697508E-622.769233
GACGTAT502.6944E-422.19999928
ACGTATA502.6944E-422.19999929
TACACAT502.6944E-422.1999995
GCGGTAA4450.021.6179823
ATCCTAC603.7121805E-521.5833321
Produced by FastQC (version 0.11.5)