##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632496.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262165
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.887353384319034	34.0	31.0	34.0	31.0	34.0
2	33.030332042797475	34.0	33.0	34.0	31.0	34.0
3	33.11024736330174	34.0	33.0	34.0	31.0	34.0
4	36.451662121183226	37.0	37.0	37.0	35.0	37.0
5	36.4033566646959	37.0	37.0	37.0	35.0	37.0
6	36.43773577708695	37.0	37.0	37.0	35.0	37.0
7	36.45030038334637	37.0	37.0	37.0	35.0	37.0
8	36.42583868937501	37.0	37.0	37.0	35.0	37.0
9	38.186985295519996	39.0	39.0	39.0	37.0	39.0
10	38.196658592870904	39.0	38.0	39.0	37.0	39.0
11	38.27509011500391	39.0	39.0	39.0	37.0	39.0
12	38.22283294871551	39.0	39.0	39.0	37.0	39.0
13	38.2690481185513	39.0	39.0	39.0	37.0	39.0
14	39.74886426487136	41.0	40.0	41.0	38.0	41.0
15	39.75388400434841	41.0	40.0	41.0	38.0	41.0
16	39.69016840539355	41.0	40.0	41.0	38.0	41.0
17	39.699128411496574	41.0	40.0	41.0	38.0	41.0
18	39.716159670436554	41.0	40.0	41.0	38.0	41.0
19	39.72199187534568	41.0	40.0	41.0	38.0	41.0
20	39.703755268628534	41.0	40.0	41.0	38.0	41.0
21	39.657456182175345	41.0	40.0	41.0	37.0	41.0
22	39.62054049930387	41.0	40.0	41.0	37.0	41.0
23	39.58177865084966	41.0	40.0	41.0	37.0	41.0
24	39.559903114450826	41.0	39.0	41.0	37.0	41.0
25	39.51016344668434	41.0	39.0	41.0	37.0	41.0
26	39.41787423950566	41.0	39.0	41.0	37.0	41.0
27	39.292319722312286	40.0	39.0	41.0	36.0	41.0
28	39.25847081036752	40.0	39.0	41.0	36.0	41.0
29	39.22363397097248	40.0	39.0	41.0	36.0	41.0
30	39.182175347586444	40.0	39.0	41.0	36.0	41.0
31	39.12040127400683	40.0	39.0	41.0	35.0	41.0
32	39.062773444204986	40.0	39.0	41.0	35.0	41.0
33	38.99057082371789	40.0	39.0	41.0	35.0	41.0
34	38.95633284381973	40.0	39.0	41.0	35.0	41.0
35	38.857841435737036	40.0	38.0	41.0	35.0	41.0
36	38.800534014837986	40.0	38.0	41.0	35.0	41.0
37	38.733557110979724	40.0	38.0	41.0	35.0	41.0
38	38.66297942135678	40.0	38.0	41.0	35.0	41.0
39	38.54542368355806	40.0	38.0	41.0	35.0	41.0
40	38.46790761543303	40.0	38.0	41.0	35.0	41.0
41	38.40431026262087	40.0	38.0	41.0	35.0	41.0
42	38.318124082161994	40.0	37.0	41.0	35.0	41.0
43	37.55946445940533	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	0.0
18	8.0
19	5.0
20	13.0
21	41.0
22	64.0
23	112.0
24	181.0
25	232.0
26	354.0
27	500.0
28	712.0
29	959.0
30	1333.0
31	1761.0
32	2267.0
33	3332.0
34	4796.0
35	7629.0
36	12547.0
37	24427.0
38	65416.0
39	135474.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.99429748440867	16.189422691816223	12.754181526900998	28.062098296874105
2	22.32067591020922	19.594148723132378	31.31386722102493	26.771308145633476
3	22.15207979707436	19.136040279976353	28.697194514904734	30.01468540804455
4	16.166154902446934	16.20925752865562	34.0064463219728	33.61814124692465
5	17.84067285869586	32.241527282436635	32.14693036827952	17.770869490587987
6	33.29277363492457	33.58228596494574	15.78853012415845	17.33641027597124
7	29.85333663913947	26.952873190547937	19.35155341101978	23.842236759292813
8	26.640855949497457	29.99408769286518	20.70261095111857	22.662445406518795
9	27.652814067476587	12.67179066618351	17.4722026204871	42.2031926458528
10	20.24755402132245	22.356149753018137	28.4721454046116	28.92415082104781
11	39.892052714893296	19.31607956821086	18.532222073884768	22.25964564301108
12	22.36034558388801	23.13314134228444	26.8426372704213	27.663875803406253
13	36.718478820590086	16.895848034634678	20.158297255545172	26.227375889230064
14	24.25533538039021	19.96490759636107	22.634981786279635	33.14477523696908
15	30.502164667289684	23.25634619419068	19.22033833654378	27.021150801975857
16	25.324127934697614	23.614517574809756	22.606373848530506	28.454980641962123
17	27.504052791181127	23.055327751606814	21.46815936528522	27.972460091926838
18	27.05738752312475	20.328037686189994	22.905422157801382	29.70915263288387
19	28.935212556977476	22.211965746762534	22.604085213510576	26.248736482749415
20	30.584555528007172	20.334903591249788	21.77903228882574	27.301508591917305
21	28.229550092499	21.28430568535083	21.537962733393094	28.94818148875708
22	28.606793431617493	22.295882364159976	20.67934316174928	28.41798104247325
23	29.230065035378484	21.15347205004482	21.82327923254439	27.793183682032307
24	28.302786413136765	20.76745561001659	22.104399900825815	28.825358076020823
25	29.29033242423664	21.85303148780348	21.96250452959014	26.89413155836973
26	28.967253447256496	22.274521770640625	21.68100242213873	27.077222359964143
27	27.57194896343906	21.500581694734233	24.012740068277612	26.9147292735491
28	26.99979020845651	23.33606698071825	21.259893578471573	28.404249232353667
29	27.485743711021687	23.51763202563271	21.516220700703755	27.480403562641847
30	27.375889230065038	22.07998779394656	23.586672515400604	26.957450460587793
31	28.593061621497913	21.95144279366048	21.925504930101273	27.52999065474033
32	25.74065950832491	21.610436175690882	22.935174413060476	29.71372990292373
33	26.469589762172678	21.487231323784638	24.07415177464574	27.969027139396946
34	26.505063604981594	21.463200656075372	23.74916560181565	28.282570137127383
35	26.17740735796159	23.03701867144737	23.99519386645815	26.790380104132893
36	26.531001468540804	21.32359391985963	25.073903839185245	27.07150077241432
37	27.370930520855186	20.640817805580454	24.16417141876299	27.824080254801366
38	25.72196898899548	19.928670875212177	26.17817023630157	28.171189899490777
39	25.199778765281405	18.966299849331527	27.39610550607442	28.437815879312645
40	24.945740278069156	20.000762878339977	28.42332119085309	26.63017565273778
41	23.170140941773308	19.411439360707952	29.41239295863292	28.006026738885815
42	21.43726279251616	20.360460015639006	30.521999504129077	27.680277687715755
43	20.394026662597984	20.963133904220623	29.76484275170217	28.877996681479225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	3.0
3	4.5
4	6.0
5	6.0
6	10.0
7	14.0
8	13.0
9	12.0
10	20.0
11	28.0
12	28.0
13	56.5
14	85.0
15	159.5
16	234.0
17	229.5
18	225.0
19	225.0
20	272.0
21	319.0
22	291.5
23	264.0
24	320.0
25	376.0
26	376.0
27	484.0
28	592.0
29	794.5
30	997.0
31	1208.5
32	1420.0
33	1420.0
34	1856.5
35	2293.0
36	2457.5
37	2622.0
38	3565.5
39	4509.0
40	4509.0
41	5683.5
42	6858.0
43	8408.5
44	9959.0
45	14120.0
46	18281.0
47	18281.0
48	21148.0
49	24015.0
50	26670.5
51	29326.0
52	30177.5
53	31029.0
54	31029.0
55	29373.5
56	27718.0
57	26435.5
58	25153.0
59	23586.5
60	22020.0
61	22020.0
62	20206.0
63	18392.0
64	15026.0
65	11660.0
66	10049.0
67	8438.0
68	8438.0
69	7234.5
70	6031.0
71	5091.5
72	4152.0
73	3224.5
74	2297.0
75	2297.0
76	1814.0
77	1331.0
78	1038.5
79	746.0
80	565.5
81	385.0
82	385.0
83	293.0
84	201.0
85	144.0
86	87.0
87	69.5
88	52.0
89	52.0
90	40.5
91	29.0
92	17.0
93	5.0
94	2.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	262165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.574071259725386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.35911972940225	36.710812717066304
2	8.466480836727474	7.547710402707796
3	2.6089680287381762	3.488769804819622
4	1.3833942778839163	2.466540604907761
5	0.9452739303214691	2.1067353765054926
6	0.6013483862784252	1.6082727493137237
7	0.4711197748979467	1.4699808492716804
8	0.3788233000346919	1.3508557416472549
9	0.2786508774798971	1.1178543662436546
>10	2.0843901195117347	17.83097460340611
>50	0.23104779841009843	7.150331916488853
>100	0.1864248330519249	15.513528441919128
>500	0.003966485809615424	1.156729840233129
>1k	9.91621452403856E-4	0.48090258546948583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1088	0.4150058169473423	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	819	0.31239868022047185	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	651	0.24831689966242632	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	607	0.2315335761829382	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	540	0.20597715179371767	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	477	0.18194648408445063	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	465	0.17736921404459025	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	460	0.1754620181946484	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	445	0.16974043064482291	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	429	0.16363740392500906	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	428	0.16325596475502072	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	408	0.15562718135525336	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	382	0.14570976293555585	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	376	0.14342112791562567	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	359	0.1369366620258234	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	347	0.13235939198596303	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	347	0.13235939198596303	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	341	0.13007075696603285	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	338	0.12892643945606774	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	337	0.12854500028607938	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	337	0.12854500028607938	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	332	0.12663780443613756	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	331	0.1262563652661492	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	330	0.12587492609616083	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	323	0.12320485190624225	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	322	0.12282341273625388	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	315	0.12015333854633531	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	309	0.11786470352640513	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	307	0.1171018251864284	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	306	0.11672038601644004	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	303	0.11557606850647494	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	299	0.11405031182652146	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	290	0.11061735929662617	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	288	0.10985448095664944	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	279	0.10642152842675415	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	278	0.10604008925676578	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	276	0.10527721091678904	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	273	0.10413289340682394	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	268	0.1022256975568821	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	266	0.10146281921690539	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	263	0.10031850170694029	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	3.814391699883661E-4
8	0.0	0.0	0.0	0.0	3.814391699883661E-4
9	0.0	0.0	0.0	0.0	3.814391699883661E-4
10	0.0	0.0	0.0	0.0	3.814391699883661E-4
11	0.0	0.0	0.0	0.0	3.814391699883661E-4
12	0.0	0.0	0.0	0.0	3.814391699883661E-4
13	0.0	0.0	0.0	0.0	3.814391699883661E-4
14	0.0	0.0	0.0	0.0	3.814391699883661E-4
15	0.0	0.0	0.0	0.0	3.814391699883661E-4
16	0.0	0.0	0.0	0.0	3.814391699883661E-4
17	0.0	0.0	0.0	3.814391699883661E-4	3.814391699883661E-4
18	0.0	0.0	0.0	3.814391699883661E-4	3.814391699883661E-4
19	0.0	0.0	0.0	3.814391699883661E-4	3.814391699883661E-4
20	0.0	0.0	0.0	7.628783399767322E-4	7.628783399767322E-4
21	0.0	0.0	0.0	0.002670074189918563	7.628783399767322E-4
22	0.0	0.0	0.0	0.003814391699883661	7.628783399767322E-4
23	0.0	0.0	0.0	0.003814391699883661	7.628783399767322E-4
24	0.0	0.0	0.0	0.007247344229778956	7.628783399767322E-4
25	0.0	0.0	0.0	0.008391661739744054	0.0011443175099650983
26	0.0	0.0	0.0	0.01373181011958118	0.0011443175099650983
27	0.0	0.0	0.0	0.05187572711841779	0.0011443175099650983
28	0.0	0.0	0.0	0.20750290847367117	0.0011443175099650983
29	0.0	0.0	0.0	0.39784105429786587	0.0015257566799534645
30	0.0	0.0	0.0	0.648065149810234	0.0015257566799534645
31	0.0	0.0	0.0	1.3018518871702935	0.0015257566799534645
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGC	35	5.6092176E-7	37.0	1
GGTATCA	415	0.0	33.879517	1
TTTTACG	30	3.5919694E-4	30.833332	4
ATACACA	45	3.992758E-6	28.777779	37
TCCTACA	55	6.2308027E-7	26.90909	2
CAAGACA	35	8.851067E-4	26.428572	4
TACGGCT	110	3.6379788E-12	23.545454	30
GTATCAA	585	0.0	23.401709	2
TTTACGA	40	0.0019272342	23.125002	5
CTTTTTA	40	0.0019272342	23.125002	2
GGACGTA	40	0.0019272342	23.125002	27
ACTGTGC	40	0.0019272342	23.125002	8
TTACGAG	40	0.0019272342	23.125002	6
ATACGGC	105	4.0017767E-11	22.90476	29
CCTACAC	65	2.6697508E-6	22.76923	3
GACGTAT	50	2.6944E-4	22.199999	28
ACGTATA	50	2.6944E-4	22.199999	29
TACACAT	50	2.6944E-4	22.199999	5
GCGGTAA	445	0.0	21.61798	23
ATCCTAC	60	3.7121805E-5	21.583332	1
>>END_MODULE