FastQCFastQC Report
Fri 10 Feb 2017
ERR1632495.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632495.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226747
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7650.33738042840699106No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5890.2597608788649905No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4740.2090435595619788No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4640.20463335788345602No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4230.1865515310015127No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4120.18170030915513766No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4000.17640806714091037No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3840.16935174445527393No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3760.16582358311245574No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT3710.16361848227319434No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3460.15259297807688746No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3450.15215195790903518No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3390.1495058369019215No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3380.14906481673406927No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3360.1481827763983647No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3150.13892135287346688No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3150.13892135287346688No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3140.13848033270561463No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3000.13230605035568277No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2980.13142401001997822No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC2940.1296599293485691No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA2730.1203985058236713No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2660.1173113646487054No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2630.11598830414514855No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2600.11466524364159172No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2530.11157810246662579No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2510.11069606213092126No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2480.10937300162736441No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2480.10937300162736441No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2480.10937300162736441No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2480.10937300162736441No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2460.10849096129165987No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2270.10011157810246663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACCG200.00183882137.05
TGGGCTA303.5904502E-430.8333325
TACGGCT551.8419087E-830.27272830
GGTATCA3250.029.030771
ATACGGC604.2906322E-827.74999829
TTAGAAC556.2245454E-726.909093
CTCCCAT358.847358E-426.428571
CTTGTCG451.3186401E-424.66666621
CGAAATT451.3186401E-424.66666613
GCGAAAT451.3186401E-424.66666612
CGTTAGA451.3186401E-424.6666661
ATCTAAG652.6671041E-622.7692332
CATCTAA652.6671041E-622.7692331
TAGCGAA502.6928412E-422.210
TTGTCGG502.6928412E-422.222
ATGGTGT502.6928412E-422.26
TAGAACA705.072172E-621.1428574
GTATCAA4350.020.839082
TGGTGTG450.003816483320.5555557
TAGGACC450.003816483320.5555554
Produced by FastQC (version 0.11.5)