##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632495.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	226747
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02688458943228	34.0	33.0	34.0	31.0	34.0
2	33.16294372141638	34.0	33.0	34.0	31.0	34.0
3	33.238481655766115	34.0	34.0	34.0	31.0	34.0
4	36.5256563482648	37.0	37.0	37.0	35.0	37.0
5	36.494489453002686	37.0	37.0	37.0	35.0	37.0
6	36.545705125095374	37.0	37.0	37.0	35.0	37.0
7	36.547491256775174	37.0	37.0	37.0	35.0	37.0
8	36.526220854079654	37.0	37.0	37.0	35.0	37.0
9	38.322681226212474	39.0	39.0	39.0	37.0	39.0
10	38.3515063043833	39.0	39.0	39.0	37.0	39.0
11	38.41872659836734	39.0	39.0	39.0	37.0	39.0
12	38.37869960793307	39.0	39.0	39.0	37.0	39.0
13	38.415564483763845	39.0	39.0	39.0	37.0	39.0
14	39.95020882304948	41.0	40.0	41.0	38.0	41.0
15	39.94969723965477	41.0	40.0	41.0	38.0	41.0
16	39.90712115265031	41.0	40.0	41.0	38.0	41.0
17	39.907235817893955	41.0	40.0	41.0	38.0	41.0
18	39.92626583813678	41.0	40.0	41.0	38.0	41.0
19	39.92146312850887	41.0	40.0	41.0	38.0	41.0
20	39.91124910142141	41.0	40.0	41.0	38.0	41.0
21	39.86059793514357	41.0	40.0	41.0	38.0	41.0
22	39.83192280382982	41.0	40.0	41.0	38.0	41.0
23	39.80145713063458	41.0	40.0	41.0	38.0	41.0
24	39.786458034725925	41.0	40.0	41.0	38.0	41.0
25	39.75200553921331	41.0	40.0	41.0	38.0	41.0
26	39.66631973080129	41.0	40.0	41.0	37.0	41.0
27	39.57132398664591	41.0	40.0	41.0	37.0	41.0
28	39.540236475014005	41.0	40.0	41.0	37.0	41.0
29	39.50188095101589	41.0	40.0	41.0	37.0	41.0
30	39.46446038977363	41.0	39.0	41.0	37.0	41.0
31	39.390937917590975	41.0	39.0	41.0	36.0	41.0
32	39.35383489086956	41.0	39.0	41.0	36.0	41.0
33	39.28803909202768	41.0	39.0	41.0	36.0	41.0
34	39.25376741478388	41.0	39.0	41.0	36.0	41.0
35	39.18936964987409	41.0	39.0	41.0	35.0	41.0
36	39.13742188430277	41.0	39.0	41.0	35.0	41.0
37	39.087767423604284	41.0	39.0	41.0	35.0	41.0
38	39.0229154079216	41.0	39.0	41.0	35.0	41.0
39	38.92223932400429	40.0	39.0	41.0	35.0	41.0
40	38.84298358963955	40.0	38.0	41.0	35.0	41.0
41	38.79729831045174	40.0	38.0	41.0	35.0	41.0
42	38.73199645419785	40.0	38.0	41.0	35.0	41.0
43	38.008454356617726	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	2.0
18	2.0
19	3.0
20	9.0
21	19.0
22	32.0
23	79.0
24	130.0
25	173.0
26	271.0
27	368.0
28	498.0
29	711.0
30	940.0
31	1231.0
32	1691.0
33	2348.0
34	3484.0
35	5601.0
36	8616.0
37	16662.0
38	44189.0
39	139687.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.71809549850715	16.410360445783187	12.663453099710251	28.208090955999417
2	21.63777249533621	19.363872509889877	31.89590159958015	27.10245339519376
3	21.581762934018972	19.254940528430364	28.977230128733787	30.186066408816874
4	15.736040609137056	15.940232946852658	34.62625745875359	33.6974689852567
5	17.5080596435675	32.4590843539275	32.866586989023006	17.166269013481987
6	33.303637975364616	33.906512544818675	15.798665472972079	16.991184006844634
7	29.632145077994416	27.41028547235465	19.70566313997539	23.25190630967554
8	26.86694862556065	30.15210785589225	20.862018020084058	22.118925498463042
9	27.99772433593388	12.517034403983294	17.607730201502115	41.87751105858071
10	19.54910098038783	22.683872333481812	29.098510674893163	28.668516011237195
11	39.89159724274191	19.217894834330775	18.505647263249347	22.384860659677965
12	22.442193281498763	23.65852690443534	26.922076146542178	26.977203667523714
13	35.97180999087088	17.336061778105112	20.13521678346351	26.5569114475605
14	23.975620405121127	20.27766629767979	23.003611955174712	32.743101342024374
15	29.922336348441213	23.992820191667366	19.508086104777572	26.576757355113852
16	25.251491750717758	23.890062492557785	22.70460028137087	28.153845475353588
17	27.121417262411413	23.513872289379794	21.566768248311995	27.797942199896802
18	26.77653949115093	20.904355956197875	23.13327188452328	29.185832668127915
19	28.26454153748451	22.4492496041844	22.981560946782096	26.304647911548994
20	30.625763516165595	20.48053557489184	22.160381394241156	26.73331951470141
21	27.702240823472856	21.64174167684688	21.836231570869735	28.819785928810525
22	28.65749050704089	22.607134824275516	21.060918115785434	27.674456552898164
23	28.6702800919086	21.51737398951254	22.020136980864137	27.792208937714726
24	28.018893303990794	21.180875601441254	22.55200730329398	28.248223791273976
25	28.300264171080542	22.076146542181373	22.44351634200232	27.180072944735763
26	28.406991051700793	22.527751194062105	22.112751216113114	26.952506538123988
27	27.250195151424272	21.545599280255086	24.502639505704597	26.701566062616045
28	26.655699965159407	23.01551949970672	21.727299589410222	28.601480945723644
29	27.424839137893777	23.6025173431181	21.830057288519804	27.142586230468318
30	27.15405275483248	21.796980775930884	23.792597035462432	27.256369433774207
31	28.276008061848668	22.130392022827202	22.497761822648148	27.09583809267598
32	25.52624731528973	21.891800112019123	22.982884007285655	29.599068565405496
33	26.05238437553749	21.640418616343325	24.36812835450965	27.939068653609528
34	26.25348957207813	21.46489258953812	24.102634213462583	28.178983624921166
35	25.81246940422586	23.01155031819605	24.630535354381756	26.54544492319634
36	26.214679797307134	21.30656634927915	25.362628833016533	27.11612502039718
37	26.571465113099624	20.801157236920446	25.050827574344975	27.57655007563496
38	25.387325962416263	20.088468645671167	26.576316334946	27.947889056966574
39	24.815322804711858	18.982390064697658	28.100482035043463	28.101805095547018
40	24.561295188028947	20.175349618738064	28.95076891866265	26.312586274570336
41	22.7015131401959	19.429143494732017	29.725200333411244	28.14414303166084
42	20.836438850348628	20.38174705729293	31.165572201616783	27.616241890741666
43	19.77975452817457	21.151327250195152	30.37746916166476	28.691449059965514
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	7.0
4	13.0
5	13.0
6	13.0
7	13.0
8	14.5
9	16.0
10	17.0
11	18.0
12	18.0
13	46.0
14	74.0
15	131.5
16	189.0
17	183.5
18	178.0
19	178.0
20	220.5
21	263.0
22	228.0
23	193.0
24	272.5
25	352.0
26	352.0
27	436.5
28	521.0
29	659.5
30	798.0
31	1026.5
32	1255.0
33	1255.0
34	1614.0
35	1973.0
36	2333.5
37	2694.0
38	3527.5
39	4361.0
40	4361.0
41	5294.0
42	6227.0
43	7515.5
44	8804.0
45	12895.0
46	16986.0
47	16986.0
48	19365.0
49	21744.0
50	24262.5
51	26781.0
52	27321.5
53	27862.0
54	27862.0
55	25876.0
56	23890.0
57	23038.5
58	22187.0
59	20455.5
60	18724.0
61	18724.0
62	16706.0
63	14688.0
64	11816.0
65	8944.0
66	7579.0
67	6214.0
68	6214.0
69	5297.0
70	4380.0
71	3654.0
72	2928.0
73	2215.0
74	1502.0
75	1502.0
76	1191.5
77	881.0
78	671.5
79	462.0
80	386.5
81	311.0
82	311.0
83	239.0
84	167.0
85	128.0
86	89.0
87	61.5
88	34.0
89	34.0
90	28.0
91	22.0
92	12.5
93	3.0
94	2.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	226747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.949866164332384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.5718121029864	41.3977888371625
2	7.09418347426896	6.945186632213683
3	2.351172618922237	3.4526875507645918
4	1.3500377376891668	2.6433666630673116
5	0.8067449357425128	1.9745028316674467
6	0.5692542249019253	1.6718950873458007
7	0.4286618393041986	1.4688057764587952
8	0.3269756397859871	1.280433104521682
9	0.28510616853325366	1.2560317913095522
>10	1.8741234250645378	18.10363375841352
>50	0.1993748296214774	6.726158649309238
>100	0.14055925488314158	12.418799885731293
>500	0.001993748296214774	0.6607094320345843
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	765	0.33738042840699106	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	589	0.2597608788649905	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	474	0.2090435595619788	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	464	0.20463335788345602	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	423	0.1865515310015127	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	412	0.18170030915513766	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	400	0.17640806714091037	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	384	0.16935174445527393	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	376	0.16582358311245574	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	371	0.16361848227319434	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	346	0.15259297807688746	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	345	0.15215195790903518	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	339	0.1495058369019215	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	338	0.14906481673406927	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	336	0.1481827763983647	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	315	0.13892135287346688	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	315	0.13892135287346688	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	314	0.13848033270561463	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	300	0.13230605035568277	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	298	0.13142401001997822	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	294	0.1296599293485691	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	273	0.1203985058236713	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	266	0.1173113646487054	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	263	0.11598830414514855	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	260	0.11466524364159172	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	253	0.11157810246662579	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	251	0.11069606213092126	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	248	0.10937300162736441	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	248	0.10937300162736441	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	248	0.10937300162736441	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	248	0.10937300162736441	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	246	0.10849096129165987	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	227	0.10011157810246663	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.410201678522759E-4
6	0.0	0.0	0.0	0.0	4.410201678522759E-4
7	0.0	0.0	0.0	0.0	4.410201678522759E-4
8	0.0	0.0	0.0	0.0	4.410201678522759E-4
9	0.0	0.0	0.0	0.0	4.410201678522759E-4
10	0.0	0.0	0.0	0.0	4.410201678522759E-4
11	0.0	0.0	0.0	0.0	4.410201678522759E-4
12	0.0	0.0	0.0	0.0	4.410201678522759E-4
13	0.0	0.0	0.0	0.0	4.410201678522759E-4
14	0.0	0.0	0.0	0.0	4.410201678522759E-4
15	0.0	0.0	0.0	0.0	4.410201678522759E-4
16	0.0	0.0	0.0	0.0	4.410201678522759E-4
17	0.0	0.0	0.0	0.0	4.410201678522759E-4
18	0.0	0.0	0.0	0.0	4.410201678522759E-4
19	0.0	0.0	0.0	4.410201678522759E-4	4.410201678522759E-4
20	0.0	0.0	0.0	8.820403357045518E-4	4.410201678522759E-4
21	0.0	0.0	0.0	0.0030871411749659313	4.410201678522759E-4
22	0.0	0.0	0.0	0.0030871411749659313	4.410201678522759E-4
23	0.0	0.0	0.0	0.00529224201422731	4.410201678522759E-4
24	0.0	0.0	0.0	0.00749734285348869	4.410201678522759E-4
25	0.0	0.0	0.0	0.008820403357045517	4.410201678522759E-4
26	0.0	0.0	0.0	0.014112645371272829	4.410201678522759E-4
27	0.0	0.0	0.0	0.04321997644952304	4.410201678522759E-4
28	0.0	0.0	0.0	0.166264603280308	4.410201678522759E-4
29	0.0	0.0	0.0	0.37751326368154814	4.410201678522759E-4
30	0.0	0.0	0.0	0.6522688282535161	4.410201678522759E-4
31	0.0	0.0	0.0	1.4522794127375445	4.410201678522759E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACCG	20	0.001838821	37.0	5
TGGGCTA	30	3.5904502E-4	30.833332	5
TACGGCT	55	1.8419087E-8	30.272728	30
GGTATCA	325	0.0	29.03077	1
ATACGGC	60	4.2906322E-8	27.749998	29
TTAGAAC	55	6.2245454E-7	26.90909	3
CTCCCAT	35	8.847358E-4	26.42857	1
CTTGTCG	45	1.3186401E-4	24.666666	21
CGAAATT	45	1.3186401E-4	24.666666	13
GCGAAAT	45	1.3186401E-4	24.666666	12
CGTTAGA	45	1.3186401E-4	24.666666	1
ATCTAAG	65	2.6671041E-6	22.76923	32
CATCTAA	65	2.6671041E-6	22.76923	31
TAGCGAA	50	2.6928412E-4	22.2	10
TTGTCGG	50	2.6928412E-4	22.2	22
ATGGTGT	50	2.6928412E-4	22.2	6
TAGAACA	70	5.072172E-6	21.142857	4
GTATCAA	435	0.0	20.83908	2
TGGTGTG	45	0.0038164833	20.555555	7
TAGGACC	45	0.0038164833	20.555555	4
>>END_MODULE