FastQCFastQC Report
Fri 10 Feb 2017
ERR1632494.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632494.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201131
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA10310.5126012399878686No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8060.40073385007781No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7040.3500206333185834No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6110.3037821121557592No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5690.28290019937254823No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5400.2684817357841407No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5200.25853796779213545No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5020.2495885765993308No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4930.24511388100292844No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4810.2391476202077253No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4770.23715886660932425No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4740.2356673014105235No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4720.23467292461132297No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4640.2306954174145209No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4570.22721509861731906No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4530.22522634501891803No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4500.22373477982011725No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4350.21627695382611334No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4310.2142882002277123No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4230.21031069303091018No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4160.20683037423370837No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3990.19837817144050396No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3880.19290909904490108No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3860.19191472224570058No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3690.18346251945249614No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3670.1824681426532956No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3600.1789878238560938No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3500.17401593986009117No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3470.17252437466129042No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3330.16556373706688676No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3300.16407217186808598No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3200.15910028787208338No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3090.1536312154764805No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3010.14965370827967842No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2980.14816214308087763No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2960.1471677662816771No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2940.1461733894824766No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2940.1461733894824766No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2900.14418463588407557No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2890.1436874474844753No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA2880.143190259084875No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2860.1421958822856745No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2820.14020712868727347No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2780.1382183750888724No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2770.13772118668927216No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2700.13424086789207035No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2670.13274930269326957No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2570.12777741869726694No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2510.1247942882996654No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2510.1247942882996654No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC2500.12429709990006513No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2490.12379991150046486No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2490.12379991150046486No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT2460.1223083463016641Illumina PCR Primer Index 10 (95% over 22bp)
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2450.12181115790206383No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC2430.12081678110286331No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2400.11932521590406252No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2370.11783365070526175No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2310.11485052030766017No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2280.1133589551088594No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2260.11236457830965887No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2240.11137020151045837No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2220.11037582471125783No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2220.11037582471125783No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2210.10987863631165756No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2200.10938144791205731No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC2170.10788988271325653No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2150.10689550591405601No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2140.10639831751445576No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2120.10540394071525522No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2090.10391237551645445No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2060.10242081031765367No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2020.10043205671925262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATGC251.230007E-436.99999632
CAATGCT303.5890198E-430.83333233
GGCGGGT250.005485729.59999827
GGTATCA4100.027.975611
ATACGGC905.456968E-1226.72222129
CTAAGTC358.8438677E-426.4285734
TACGGCT1000.025.89999830
ATACACA659.311407E-825.61538537
AATGCTG451.317916E-424.66666634
ACGGCTG1103.6379788E-1223.54545431
ATATGCC400.001925681723.12535
AATCTGA400.001925681723.12525
ATCTGAG400.001925681723.12526
ATCTAAG903.781679E-922.6111132
TTGATAT502.691375E-422.19999932
ATTGATA502.691375E-422.19999931
TCTAAGT957.057679E-921.42105333
TCACAAT705.067468E-621.14285733
TAAGTCT450.003815005820.55555535
TATTGAT450.003815005820.55555530
Produced by FastQC (version 0.11.5)