FastQCFastQC Report
Fri 10 Feb 2017
ERR1632493.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632493.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14563
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA820.5630707958525029No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA690.4738034745588134No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA630.43260317242326446No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA580.3982695873103069No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT530.36393600219734945RNA PCR Primer, Index 26 (100% over 22bp)
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT530.36393600219734945No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT500.34333585112957493No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT480.329602417084392No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC440.30213554899402595No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA420.28840211494884294No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC410.28153539792625143No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA400.27466868090366No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC400.27466868090366No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT400.27466868090366No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG400.27466868090366No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA400.27466868090366No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA400.27466868090366No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA360.24720181281329395No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT360.24720181281329395No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT340.23346837876811097No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC330.22660166174551946No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA330.22660166174551946No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC320.21973494472292796No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT320.21973494472292796No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA320.21973494472292796No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG300.20600151067774497No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG290.19913479365515346No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC290.19913479365515346No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA270.18540135960997048No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC270.18540135960997048No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC260.17853464258737897No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC260.17853464258737897No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC260.17853464258737897No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG260.17853464258737897No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA250.17166792556478747No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT240.164801208542196No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT240.164801208542196No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA240.164801208542196No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATC240.164801208542196No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG240.164801208542196No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA240.164801208542196No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT240.164801208542196No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC230.15793449151960448No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA230.15793449151960448No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC230.15793449151960448No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA220.15106777449701297No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC220.15106777449701297No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA210.14420105747442147No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG210.14420105747442147No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA210.14420105747442147No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA210.14420105747442147No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC210.14420105747442147No Hit
GAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAA200.13733434045183No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC200.13733434045183No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC200.13733434045183No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA200.13733434045183No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC200.13733434045183No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG200.13733434045183No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC200.13733434045183No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC200.13733434045183No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG200.13733434045183No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG200.13733434045183No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG190.13046762342923848No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA190.13046762342923848No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC190.13046762342923848No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA190.13046762342923848No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG190.13046762342923848No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA190.13046762342923848No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA180.12360090640664698No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG180.12360090640664698No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG180.12360090640664698No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG180.12360090640664698No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT180.12360090640664698No Hit
TTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCAT180.12360090640664698No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC180.12360090640664698No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG180.12360090640664698No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA170.11673418938405548No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA170.11673418938405548No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC170.11673418938405548No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA170.11673418938405548No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT170.11673418938405548No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT170.11673418938405548No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG160.10986747236146398No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT160.10986747236146398No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC160.10986747236146398No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA160.10986747236146398No Hit
GTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTACA160.10986747236146398No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG160.10986747236146398No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC160.10986747236146398No Hit
GGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACT160.10986747236146398No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC160.10986747236146398No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC150.10300075533887249No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT150.10300075533887249No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT150.10300075533887249No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT150.10300075533887249No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC150.10300075533887249No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG150.10300075533887249No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT150.10300075533887249No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG150.10300075533887249No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC150.10300075533887249No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT150.10300075533887249No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG150.10300075533887249No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAACAT250.00532204729.627
AAACATC250.00532204729.628
CTGAAAC250.00532204729.625
TGAAACA250.00532204729.626
AACATCT250.00532204729.629
GGTATCA453.6630372E-628.7777771
GCGGTAA405.5531003E-527.7523
GCAGCCG405.5531003E-527.7517
CGCGGTA405.5531003E-527.7522
CCGCGGT405.5531003E-527.7521
CAGCCGC405.5531003E-527.7518
CAGCAGC451.237669E-424.66666615
CCAGCAG451.237669E-424.66666614
AACTCCG451.237669E-424.6666665
TAACTCC451.237669E-424.6666664
CTAACTC451.237669E-424.6666663
GCTAACT451.237669E-424.6666662
AGCCGCG451.237669E-424.66666619
GTAATAC451.237669E-424.66666626
GGTAATA451.237669E-424.66666625
Produced by FastQC (version 0.11.5)