FastQCFastQC Report
Fri 10 Feb 2017
ERR1632492.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632492.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35168
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2270.6454731574158326No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1380.3924021838034577No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT1350.3838717015468608TruSeq Adapter, Index 12 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1270.36112374886260235No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1130.3213148316651501No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1020.2900363967242948No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1000.2843494085532302No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA990.2815059144676979No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC960.27297543221110104No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC910.25875796178343946No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT900.2559144676979072No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT900.2559144676979072No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC890.25307097361237485No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC840.23885350318471338No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA840.23885350318471338No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA810.23032302092811646No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA810.23032302092811646No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA780.22179253867151957No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT770.21894904458598727No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC770.21894904458598727No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT750.21326205641492266No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA730.20757506824385805No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT700.19904458598726116No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA690.19620109190172885No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC670.19051410373066424No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG670.19051410373066424No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG650.18482711555959963No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC640.18198362147406735No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC630.17914012738853505No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC620.17629663330300274No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG610.17345313921747044No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC590.16776615104640583No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC590.16776615104640583No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA580.16492265696087352No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA580.16492265696087352No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC570.16207916287534124No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA540.15354868061874433No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC540.15354868061874433No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA540.15354868061874433No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC540.15354868061874433No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA540.15354868061874433No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT540.15354868061874433No Hit
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC520.14786169244767972No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC520.14786169244767972No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG520.14786169244767972No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC500.1421747042766151No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG500.1421747042766151No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC490.1393312101910828No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT490.1393312101910828No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG490.1393312101910828No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG490.1393312101910828No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC480.13648771610555052No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT470.13364422202001822No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC470.13364422202001822No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT460.1308007279344859No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC460.1308007279344859No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT460.1308007279344859No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA450.1279572338489536No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC450.1279572338489536No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT440.1251137397634213No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA440.1251137397634213No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG440.1251137397634213No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT430.122270245677889No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA420.11942675159235669No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT410.11658325750682438No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT410.11658325750682438No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA410.11658325750682438No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG410.11658325750682438No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA410.11658325750682438No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG390.11089626933575979No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA390.11089626933575979No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC390.11089626933575979No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG380.10805277525022748No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA370.10520928116469518No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT360.10236578707916288No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA360.10236578707916288No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC360.10236578707916288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGCT251.20944766E-437.034
GGTATCA551.7500497E-830.2727281
CTGGGGC250.00542505429.633
AGCACTG250.00542505429.65
GCACTGT250.00542505429.66
TTCTGCG453.864954E-628.77777718
CTGCGGG453.864954E-628.77777720
CTCGCCG453.864954E-628.77777723
TGCGGGT453.864954E-628.77777721
AACGTCA405.780418E-527.75000228
TCAATGA405.780418E-527.75000232
GCTACTG405.780418E-527.75000229
CGCTACT405.780418E-527.75000228
GCCGCTA405.780418E-527.75000226
ACTGGGG405.780418E-527.75000232
TGAGCAA405.780418E-527.75000236
AATGAGC405.780418E-527.75000234
CGCCGCT405.780418E-527.75000225
ATGAGCA405.780418E-527.75000235
GCACATG358.698802E-426.4285731
Produced by FastQC (version 0.11.5)