FastQCFastQC Report
Fri 10 Feb 2017
ERR1632491.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632491.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810193
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA38670.4772936818758No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA31350.3869448390692094No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA27170.3353521938599815No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA23930.2953617224537857No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA21580.2663562879462054No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19330.23858512724745834No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA19290.23809141772392503No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT18960.23401831415477548No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18930.23364803201212553No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA16470.20328489631482866No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA16440.20291461417217874No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG16140.19921179274567913No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA15930.1966198177471294No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC15710.19390441536769634No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15420.19032502132208004No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC15370.18970788441766342No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT15020.18538792608674723No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT15000.1851410713249806No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA14820.18291937846908082No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC14540.17946341180434786No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC14350.17711829156756476No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA14300.17650115466314814No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC14020.1730451879984152No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13990.17267490585576523No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC13870.17119377728516538No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT13860.17107034990428205No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC13790.17020635823809882No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA13720.1693423665719156No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC13620.16810809276308236No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG13470.16625668204983257No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC13220.16317099752774955No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT13140.16218357848068302No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG13030.16082587729096648No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12850.15860418443506671No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG12530.15465450824680044No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA12470.15391394396150054No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA12250.15119854158206747No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC12110.14947055824970099No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12070.14897684872616773No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11810.14576773682320138No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT11450.14132435111140185No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC10990.13564669159076911No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC10940.1350295546863525No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC10530.12996903207013638No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC10480.12935189516571977No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA10430.12873475826130318No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC10330.12750048445246998No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10220.12614278326275344No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC10070.12429137254950365No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC10060.12416794516862031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9990.12330395350243707No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG9880.12194625231272055No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA9850.12157597017007059No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT9750.12034169636123739No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9700.11972455945682078No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9620.11873714040975421No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA9620.11873714040975421No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC9620.11873714040975421No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG9540.11774972136268766No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9430.11639202017297114No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT9330.11515774636413792No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9190.11342976303177145No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC9140.11281262612735485No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA9090.11219548922293825No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC9000.11108464279498836No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8990.11096121541410503No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA8930.11022065112880511No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT8920.11009722374792179No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8910.10997379636703847No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8860.10935665946262188No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA8810.10873952255820526No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT8640.10664125708318882No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT8380.10343214518022249No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC8360.10318529041845587No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG8320.10269158089492257No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8250.10182758922873932No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG8130.10034646065813949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACC308.30896E-637.04
ATATGCT200.001841615537.032
TAGGACA200.001841615537.04
ATTGACT251.2333045E-436.99999631
TTAAGAC352.3874449E-531.71428529
CAACTTA352.3874449E-531.71428514
TATACCC352.3874449E-531.7142855
TAAGACT303.5985498E-430.83333230
TCAATGC751.4551915E-1129.632
TAGTTAC250.005495399229.5999987
TATTGAC250.005495399229.59999830
AGACCGT250.005495399229.5999986
AGACACT250.005495399229.59999835
GGTATCA17100.027.9122811
CCCAGTT405.9379992E-527.7524
AAGACTC358.8671275E-426.4285731
ATACACA1700.026.11764737
AACTTAA451.3227413E-424.66666615
GTCTATA451.3227413E-424.6666662
TCTATAC451.3227413E-424.6666663
Produced by FastQC (version 0.11.5)