FastQCFastQC Report
Fri 10 Feb 2017
ERR1632490.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632490.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392458
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11940.3042363768861891No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA10010.2550591400863277No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7270.1852427520906696No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT5910.15058936242859108No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT5520.14065199333431858No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC5410.13784914564106224No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5300.13504629794780587No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5200.13249825459030012No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5100.12995021123279432No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC4800.12230608116027702No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG4770.1215416681530253No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4750.12103205948152412No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4620.11771960311676663No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4610.11746479878101605No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4610.11746479878101605No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4530.11542636409501143No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4470.11389753808050797No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG4200.10701782101524239No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC4200.10701782101524239No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4180.10650821234374123No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC4130.10523419066498835No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4080.10396016898623547No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3980.1014121256287297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAAA200.001840459337.018
TACTGTT251.2321447E-437.07
GGTATCA5650.030.1238941
AAAAACC250.005491988729.624
ATACGGC901.4188117E-1024.66666629
TACGGCT1054.0017767E-1122.90476430
CTTATAC7000.021.67142937
GCGGTAA5250.021.49523723
AACTCCG5350.021.439255
AGCCGCG5500.021.19090819
CGCGGTA5350.021.09345822
TGCGGGT2900.021.05172321
CGTGCCA5500.020.85454410
GGTAATA5450.020.70642325
GTAACGT2950.020.69491626
TCCGTGC5650.020.6283198
TGGATAG450.003821386720.5555555
ACTTTGT450.003821386720.5555558
TTACAGT450.003821386720.5555555
TGGTAGC450.003821386720.55555510
Produced by FastQC (version 0.11.5)