##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632490.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392458
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.046550204098274	34.0	33.0	34.0	31.0	34.0
2	33.17878601022275	34.0	33.0	34.0	31.0	34.0
3	33.264374786601365	34.0	34.0	34.0	31.0	34.0
4	36.54602021107991	37.0	37.0	37.0	35.0	37.0
5	36.51861600476994	37.0	37.0	37.0	35.0	37.0
6	36.56215442161964	37.0	37.0	37.0	35.0	37.0
7	36.55622512472672	37.0	37.0	37.0	35.0	37.0
8	36.53288249952861	37.0	37.0	37.0	35.0	37.0
9	38.33601047755429	39.0	39.0	39.0	37.0	39.0
10	38.37103588154656	39.0	39.0	39.0	37.0	39.0
11	38.44185365058172	39.0	39.0	39.0	37.0	39.0
12	38.37838953467632	39.0	39.0	39.0	37.0	39.0
13	38.425408578752375	39.0	39.0	39.0	37.0	39.0
14	39.97206834871502	41.0	40.0	41.0	38.0	41.0
15	39.96497969209444	41.0	40.0	41.0	38.0	41.0
16	39.93663270974219	41.0	40.0	41.0	38.0	41.0
17	39.932109932782616	41.0	40.0	41.0	38.0	41.0
18	39.93661742148204	41.0	40.0	41.0	38.0	41.0
19	39.93033649460579	41.0	40.0	41.0	38.0	41.0
20	39.919114911659335	41.0	40.0	41.0	38.0	41.0
21	39.892921025944176	41.0	40.0	41.0	38.0	41.0
22	39.85892757951169	41.0	40.0	41.0	38.0	41.0
23	39.83274388596996	41.0	40.0	41.0	38.0	41.0
24	39.8112995530732	41.0	40.0	41.0	38.0	41.0
25	39.77018687349984	41.0	40.0	41.0	38.0	41.0
26	39.696331836782534	41.0	40.0	41.0	38.0	41.0
27	39.595131198752476	41.0	40.0	41.0	37.0	41.0
28	39.55321333747815	41.0	40.0	41.0	37.0	41.0
29	39.50988895627048	41.0	40.0	41.0	37.0	41.0
30	39.471454270265866	41.0	39.0	41.0	37.0	41.0
31	39.386240056260796	41.0	39.0	41.0	36.0	41.0
32	39.342467219422204	41.0	39.0	41.0	36.0	41.0
33	39.272049493194174	41.0	39.0	41.0	36.0	41.0
34	39.21790357184718	41.0	39.0	41.0	35.0	41.0
35	39.148973902939936	41.0	39.0	41.0	35.0	41.0
36	39.085744207023424	41.0	39.0	41.0	35.0	41.0
37	39.01860836063987	41.0	39.0	41.0	35.0	41.0
38	38.95048896952031	41.0	39.0	41.0	35.0	41.0
39	38.84649312792706	40.0	38.0	41.0	35.0	41.0
40	38.759798500731286	40.0	38.0	41.0	35.0	41.0
41	38.69811037104608	40.0	38.0	41.0	35.0	41.0
42	38.611624683405616	40.0	38.0	41.0	35.0	41.0
43	37.85512335077894	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	5.0
18	5.0
19	14.0
20	19.0
21	30.0
22	58.0
23	135.0
24	176.0
25	323.0
26	449.0
27	630.0
28	776.0
29	1238.0
30	1699.0
31	2243.0
32	2915.0
33	4011.0
34	6140.0
35	9329.0
36	14935.0
37	30698.0
38	76769.0
39	239859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20651381803913	16.23791590437703	11.965611606847103	27.589958670736742
2	21.167360583807692	19.309072563178738	32.44805813615724	27.075508716856326
3	21.388275942903444	18.994134404191023	28.444572412844177	31.173017240061355
4	14.772536169475458	15.508665895458876	34.52828073322496	35.190517201840706
5	17.437279912754995	32.87434578986796	32.44219763643498	17.246176660942062
6	34.67555763928879	33.865279851601954	15.46127228900927	15.997890220099986
7	31.130974524662513	27.37210096367	18.895015517584046	22.601908994083445
8	27.525747978127598	30.67181711163997	20.09081226526151	21.711622644970927
9	28.231810792492446	12.125883534034216	16.912637785444556	42.729667888028786
10	19.046878901691393	22.899265653904365	29.353714282802233	28.700141161602005
11	40.28482028650199	19.072359335266448	18.22513491889578	22.417685459335775
12	22.83938663500298	23.282236570537485	26.597750587323993	27.28062620713554
13	35.91288749369359	17.199802271835456	20.505378919527693	26.38193131494326
14	24.58326750887993	19.207915241885757	22.987427954074064	33.22138929516025
15	28.858629458438866	23.96460258167753	20.214137563764787	26.96263039611882
16	26.301667949181823	23.22745363835111	22.464824261449635	28.006054151017434
17	27.228646122642424	23.030744691151664	21.477457460416147	28.263151725789765
18	26.886698704065147	20.4852493770034	23.248602398218406	29.379449520713045
19	29.42251145345489	22.399849155833234	22.517313954614252	25.66032543609762
20	30.698061958222283	20.60500741480617	21.87928389789481	26.817646729076746
21	27.646015624601866	22.051531628862197	21.309541403156516	28.99291134337942
22	29.006670777509953	22.34914309301887	20.796875079626357	27.847311049844826
23	27.754307467295863	21.720285992386444	21.90298070111961	28.62242583919808
24	27.843743789144316	21.32533927197305	22.45539650102686	28.375520437855773
25	28.360486982046485	21.847688160261736	22.681407946837624	27.110416910854156
26	28.70166998761651	22.293595747825247	21.471087352022383	27.533646912535865
27	27.82718150732053	21.57224467331536	23.326827329293838	27.273746490070277
28	26.831915771878773	23.335745481045105	21.490962090210928	28.34137665686519
29	27.720163686305288	23.798724959103904	21.848197768933236	26.632913585657576
30	27.66512594978316	21.675695233630098	22.915318327056653	27.743860489530093
31	27.45695080747494	21.77124685953656	22.372075483236422	28.399726849752078
32	25.356089059211435	21.954196372605477	23.120435817335867	29.56927875084722
33	26.48028578854298	21.202778386477025	24.131244617258407	28.185691207721593
34	26.709100082046994	21.380377008495177	24.254569915761685	27.655952993696143
35	26.67597551839942	22.17715016638723	23.765855199792078	27.381019115421267
36	25.860601643997573	20.992310005147047	24.82074514979947	28.32634320105591
37	25.99437392026663	20.704635910084647	25.51253892136229	27.78845124828644
38	25.38182429712224	19.905059904499332	26.012719832440666	28.70039596593776
39	24.464528688420163	19.521579379194716	28.1691289258978	27.844763006487316
40	25.065357312120025	19.86250758042899	28.23435883584995	26.837776271601037
41	22.679369512151617	19.569992202987326	29.50685168858833	28.243786596272724
42	20.928863725545156	20.872042358672775	30.578813529091008	27.62028038669106
43	20.492383898404416	20.716356909529175	29.63170581310612	29.159553378960297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.5
2	1.0
3	12.0
4	23.0
5	23.0
6	26.0
7	29.0
8	30.0
9	31.0
10	57.5
11	84.0
12	84.0
13	162.0
14	240.0
15	419.5
16	599.0
17	570.5
18	542.0
19	542.0
20	608.0
21	674.0
22	618.0
23	562.0
24	692.5
25	823.0
26	823.0
27	975.0
28	1127.0
29	1422.5
30	1718.0
31	2202.5
32	2687.0
33	2687.0
34	3642.0
35	4597.0
36	5116.0
37	5635.0
38	6898.5
39	8162.0
40	8162.0
41	9489.0
42	10816.0
43	13480.0
44	16144.0
45	21111.5
46	26079.0
47	26079.0
48	29615.0
49	33151.0
50	36909.5
51	40668.0
52	43106.5
53	45545.0
54	45545.0
55	42988.5
56	40432.0
57	37528.0
58	34624.0
59	34138.5
60	33653.0
61	33653.0
62	30429.5
63	27206.0
64	21490.5
65	15775.0
66	14244.0
67	12713.0
68	12713.0
69	11220.0
70	9727.0
71	9426.0
72	9125.0
73	7316.0
74	5507.0
75	5507.0
76	3551.0
77	1595.0
78	1234.0
79	873.0
80	732.5
81	592.0
82	592.0
83	468.5
84	345.0
85	267.5
86	190.0
87	142.0
88	94.0
89	94.0
90	71.0
91	48.0
92	29.0
93	10.0
94	7.0
95	4.0
96	4.0
97	3.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	392458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.558090772475026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.77618857914372	39.93575573984708
2	6.1498828901636475	5.726536116806604
3	2.2754177401144404	3.178173170686443
4	1.1752972739623544	2.1887838866312697
5	0.761504647545355	1.7727101252039121
6	0.580477309459408	1.6215549159103917
7	0.4017892732438122	1.3094579018564525
8	0.3146179843910395	1.1718410140744917
9	0.24622903249210182	1.0317558281027381
>10	1.9095965996846402	17.44678444399797
>50	0.23253553403920738	7.675745885193498
>100	0.1715155712062239	15.36275328485677
>500	0.0038481057642422026	1.0163516808970783
>1k	0.0010994587897834864	0.5617960059353027
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1194	0.3042363768861891	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1001	0.2550591400863277	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	727	0.1852427520906696	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	591	0.15058936242859108	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	552	0.14065199333431858	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	541	0.13784914564106224	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	530	0.13504629794780587	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	520	0.13249825459030012	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	510	0.12995021123279432	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	480	0.12230608116027702	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	477	0.1215416681530253	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	475	0.12103205948152412	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	462	0.11771960311676663	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	461	0.11746479878101605	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	461	0.11746479878101605	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	453	0.11542636409501143	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	447	0.11389753808050797	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	420	0.10701782101524239	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	420	0.10701782101524239	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	418	0.10650821234374123	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	413	0.10523419066498835	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	408	0.10396016898623547	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	398	0.1014121256287297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.5480433575057716E-4	0.0	0.0	0.0	0.0
6	2.5480433575057716E-4	0.0	0.0	0.0	0.0
7	2.5480433575057716E-4	0.0	0.0	0.0	0.0
8	5.096086715011543E-4	0.0	0.0	0.0	0.0
9	5.096086715011543E-4	0.0	0.0	0.0	0.0
10	5.096086715011543E-4	0.0	0.0	0.0	0.0
11	5.096086715011543E-4	0.0	0.0	0.0	0.0
12	5.096086715011543E-4	0.0	0.0	0.0	0.0
13	5.096086715011543E-4	0.0	0.0	2.5480433575057716E-4	0.0
14	5.096086715011543E-4	0.0	0.0	2.5480433575057716E-4	0.0
15	5.096086715011543E-4	0.0	0.0	5.096086715011543E-4	0.0
16	5.096086715011543E-4	0.0	0.0	0.00178363035025404	0.0
17	5.096086715011543E-4	0.0	0.0	0.0028028476932563484	0.0
18	5.096086715011543E-4	0.0	0.0	0.0030576520290069254	0.0
19	5.096086715011543E-4	0.0	0.0	0.003822065036258657	0.0
20	5.096086715011543E-4	0.0	0.0	0.00535089105076212	0.0
21	5.096086715011543E-4	0.0	0.0	0.009937369094272508	0.0
22	5.096086715011543E-4	0.0	0.0	0.015543064480785206	0.0
23	5.096086715011543E-4	0.0	0.0	0.017326694831039244	0.0
24	5.096086715011543E-4	0.0	0.0	0.022422781546050787	0.0
25	5.096086715011543E-4	0.0	0.0	0.024206411896304826	0.0
26	5.096086715011543E-4	0.0	0.0	0.028283281268314062	0.0
27	5.096086715011543E-4	0.0	0.0	0.05784058421538101	0.0
28	5.096086715011543E-4	0.0	0.0	0.20104062090720537	0.0
29	5.096086715011543E-4	0.0	0.0	0.4329125664402306	0.0
30	5.096086715011543E-4	0.0	0.0	0.7338364869616621	0.0
31	5.096086715011543E-4	0.0	0.0	1.7665584597587514	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAAA	20	0.0018404593	37.0	18
TACTGTT	25	1.2321447E-4	37.0	7
GGTATCA	565	0.0	30.123894	1
AAAAACC	25	0.0054919887	29.6	24
ATACGGC	90	1.4188117E-10	24.666666	29
TACGGCT	105	4.0017767E-11	22.904764	30
CTTATAC	700	0.0	21.671429	37
GCGGTAA	525	0.0	21.495237	23
AACTCCG	535	0.0	21.43925	5
AGCCGCG	550	0.0	21.190908	19
CGCGGTA	535	0.0	21.093458	22
TGCGGGT	290	0.0	21.051723	21
CGTGCCA	550	0.0	20.854544	10
GGTAATA	545	0.0	20.706423	25
GTAACGT	295	0.0	20.694916	26
TCCGTGC	565	0.0	20.628319	8
TGGATAG	45	0.0038213867	20.555555	5
ACTTTGT	45	0.0038213867	20.555555	8
TTACAGT	45	0.0038213867	20.555555	5
TGGTAGC	45	0.0038213867	20.555555	10
>>END_MODULE