ERR1632489.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632489.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54283
Sequences flagged as poor quality	0
Sequence length	43
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	178	0.3279111323987252	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	130	0.239485658493451	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	123	0.22659027688226516	TruSeq Adapter, Index 11 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	108	0.19895731628686697	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	95	0.17500875043752187	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	93	0.17132435569146878	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	92	0.16948215831844224	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	87	0.16027117145330952	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	86	0.15842897408028297	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	79	0.14553359246909714	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	77	0.14184919772304405	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	75	0.13816480297699094	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	72	0.13263821085791133	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	71	0.1307960134848848	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	71	0.1307960134848848	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	66	0.12158502661975205	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	65	0.1197428292467255	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	64	0.11790063187369895	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	63	0.11605843450067241	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	59	0.10868964500856623	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	58	0.10684744763553967	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	57	0.10500525026251313	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	56	0.10316305288948657	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	56	0.10316305288948657	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	55	0.10132085551646003	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	55	0.10132085551646003	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	0.0018265225	37.0	28
TTCGTCC	20	0.0018265225	37.0	4
CCGCCTT	20	0.0018265225	37.0	17
CACCGGT	20	0.0018265225	37.0	27
GTATTCC	20	0.0018265225	37.0	32
CCACCGG	20	0.0018265225	37.0	26
GGTATCA	65	1.8189894E-12	34.153847	1
ATCTAAG	30	3.554857E-4	30.833334	32
CATCTAA	30	3.554857E-4	30.833334	31
CTAAGTC	30	3.554857E-4	30.833334	34
ACATCTA	30	3.554857E-4	30.833334	30
GTATTAG	25	0.0054508755	29.599998	1
TATTCCT	25	0.0054508755	29.599998	33
TCGTCCA	25	0.0054508755	29.599998	5
ATTCCTG	25	0.0054508755	29.599998	34
ACTGAAA	35	8.760481E-4	26.42857	24
TAAGTCT	35	8.760481E-4	26.42857	35
GCGAACC	35	8.760481E-4	26.42857	11
TCTAAGT	35	8.760481E-4	26.42857	33
TTATACA	50	8.884466E-6	25.899998	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content