##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632489.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	54283
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.94038649300886	34.0	33.0	34.0	31.0	34.0
2	33.09498369655325	34.0	33.0	34.0	31.0	34.0
3	33.16364239264595	34.0	33.0	34.0	31.0	34.0
4	36.48398209384153	37.0	37.0	37.0	35.0	37.0
5	36.43801926938452	37.0	37.0	37.0	35.0	37.0
6	36.4856032275298	37.0	37.0	37.0	35.0	37.0
7	36.48941657609196	37.0	37.0	37.0	35.0	37.0
8	36.456975480352966	37.0	37.0	37.0	35.0	37.0
9	38.23208002505388	39.0	39.0	39.0	37.0	39.0
10	38.24267265994879	39.0	39.0	39.0	37.0	39.0
11	38.31521839249857	39.0	39.0	39.0	37.0	39.0
12	38.27677173332351	39.0	39.0	39.0	37.0	39.0
13	38.314794687102776	39.0	39.0	39.0	37.0	39.0
14	39.81487758598456	41.0	40.0	41.0	38.0	41.0
15	39.81950150139086	41.0	40.0	41.0	38.0	41.0
16	39.77462557338393	41.0	40.0	41.0	38.0	41.0
17	39.775399296280604	41.0	40.0	41.0	38.0	41.0
18	39.77094117863788	41.0	40.0	41.0	38.0	41.0
19	39.783947092091445	41.0	40.0	41.0	38.0	41.0
20	39.74907429582005	41.0	40.0	41.0	38.0	41.0
21	39.7031483153105	41.0	40.0	41.0	38.0	41.0
22	39.68181566973085	41.0	40.0	41.0	38.0	41.0
23	39.619973103918355	41.0	40.0	41.0	37.0	41.0
24	39.62542600814251	41.0	40.0	41.0	37.0	41.0
25	39.56026748705856	41.0	39.0	41.0	37.0	41.0
26	39.47071827275574	41.0	39.0	41.0	37.0	41.0
27	39.368697382237535	41.0	39.0	41.0	37.0	41.0
28	39.336108910708695	41.0	39.0	41.0	36.0	41.0
29	39.28502477755467	41.0	39.0	41.0	36.0	41.0
30	39.247314997328814	40.0	39.0	41.0	36.0	41.0
31	39.18211963229741	40.0	39.0	41.0	36.0	41.0
32	39.09868651327303	40.0	39.0	41.0	35.0	41.0
33	39.031004181788035	40.0	39.0	41.0	35.0	41.0
34	38.98953631892121	40.0	39.0	41.0	35.0	41.0
35	38.904518910156035	40.0	39.0	41.0	35.0	41.0
36	38.82615183390749	40.0	38.0	41.0	35.0	41.0
37	38.787410423152735	40.0	38.0	41.0	35.0	41.0
38	38.68970027448741	40.0	38.0	41.0	35.0	41.0
39	38.59134535674152	40.0	38.0	41.0	35.0	41.0
40	38.50966232522153	40.0	38.0	41.0	35.0	41.0
41	38.46047565536171	40.0	38.0	41.0	35.0	41.0
42	38.38336127332683	40.0	38.0	41.0	35.0	41.0
43	37.62417331392886	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	10.0
22	14.0
23	26.0
24	38.0
25	56.0
26	66.0
27	91.0
28	168.0
29	180.0
30	234.0
31	371.0
32	458.0
33	625.0
34	976.0
35	1530.0
36	2496.0
37	4677.0
38	12954.0
39	29310.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.39848571375937	15.988431000497394	12.342722399277859	28.270360886465372
2	22.198478344969878	19.033583258110273	30.772064919035426	27.99587347788442
3	21.581342225005987	19.122008732015548	28.500635558093695	30.79601348488477
4	15.774736105226314	15.387874656890741	33.6366818340917	35.20070740379124
5	17.570878543927197	32.77084906876922	32.40240959416391	17.255862793139656
6	35.15465246946558	33.34745684652653	14.851795221340014	16.64609546266787
7	30.57126540537553	27.194517620617876	18.867785494537884	23.366431479468712
8	28.15798684671076	29.75517196912477	19.960208536742627	22.126632647421847
9	27.957187333050864	12.257981320118637	16.745574120811305	43.039257226019195
10	19.973103918353814	22.62402593813901	28.309047031298935	29.093823112208245
11	40.80282961516497	18.755411454783268	18.003794926588434	22.437964003463332
12	23.764346112042446	22.745610964758765	25.954718788571007	27.535324134627785
13	36.64867453899011	16.714256765469855	19.71703848350312	26.920030212036917
14	24.56201757456294	18.967264152681317	22.356907319050162	34.11381095370558
15	30.37967687858077	22.990623215371293	18.740673875799054	27.889026030248882
16	26.70265092201978	22.808245675441665	21.935044120627083	28.554059281911464
17	27.778494187867288	22.933515096807472	20.879465025882872	28.408525689442364
18	27.778494187867288	20.291804063887405	22.229795700311332	29.69990604793398
19	29.03671499364442	22.062155739365917	21.710296041117846	27.19083322587182
20	31.225245472799955	20.113110918703832	21.258957684726344	27.40268592376987
21	28.50616215021277	20.9715748945342	21.566604646021776	28.955658309231254
22	28.935394138127958	22.157950002763297	20.223642761085422	28.683013098023324
23	28.959342703977303	21.51502311957703	21.00289224987565	28.52274192657001
24	28.99434445406481	20.25127572168082	21.807932501888253	28.94644732236612
25	29.34804634968591	21.257115487353314	21.590553211871118	27.80428495108966
26	29.272516257391818	21.581342225005987	21.207376158281598	27.9387653593206
27	27.831917911685057	21.640292540942838	22.909566530958127	27.618223016413978
28	27.48558480555607	22.02162739715933	21.343698763885563	29.14908903339904
29	27.924027780336385	23.053257926054197	21.211060553027654	27.811653740581765
30	27.920343385590336	21.15395243446383	23.12878801834829	27.79691616159755
31	28.69959287438056	21.612659580347437	21.708453843744817	27.97929370152718
32	26.651069395575043	21.120792881749352	22.463754766685703	29.764382955989905
33	26.500009210986864	21.26632647421845	23.699869203986516	28.533795110808175
34	27.11714533095076	20.93104655232762	23.071679899784463	28.880128216937162
35	26.31578947368421	22.883775767735756	23.72013337508981	27.08030138349023
36	26.931083396275078	21.130003868614484	24.37964003463331	27.559272700477127
37	27.181622239006686	20.31759482710978	24.248844021148425	28.25193891273511
38	25.722601919569666	19.950997549877496	25.785236630252566	28.541163900300276
39	25.101781404859718	19.031741060737247	27.19083322587182	28.67564430853122
40	24.748079509238618	19.647034983328112	28.784333953539782	26.820551553893484
41	23.22274008437264	19.018845679126063	29.119613875430613	28.638800361070686
42	21.316065803290165	19.759409023082732	31.087080669822964	27.837444503804136
43	20.623399591032182	20.297330656006483	29.836228653537937	29.243041099423394
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	3.0
11	6.0
12	6.0
13	9.0
14	12.0
15	20.0
16	28.0
17	28.0
18	28.0
19	28.0
20	43.0
21	58.0
22	43.0
23	28.0
24	35.0
25	42.0
26	42.0
27	61.0
28	80.0
29	103.5
30	127.0
31	180.0
32	233.0
33	233.0
34	341.5
35	450.0
36	534.0
37	618.0
38	789.0
39	960.0
40	960.0
41	1175.5
42	1391.0
43	1703.0
44	2015.0
45	2773.5
46	3532.0
47	3532.0
48	4131.5
49	4731.0
50	5203.5
51	5676.0
52	5840.0
53	6004.0
54	6004.0
55	5815.0
56	5626.0
57	5493.0
58	5360.0
59	4977.5
60	4595.0
61	4595.0
62	4281.5
63	3968.0
64	3333.5
65	2699.0
66	2342.5
67	1986.0
68	1986.0
69	1705.0
70	1424.0
71	1229.0
72	1034.0
73	865.5
74	697.0
75	697.0
76	528.5
77	360.0
78	301.5
79	243.0
80	185.0
81	127.0
82	127.0
83	101.5
84	76.0
85	52.0
86	28.0
87	24.0
88	20.0
89	20.0
90	14.5
91	9.0
92	9.5
93	10.0
94	5.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	54283.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.61173848166092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.45095567617626	64.7735018329864
2	4.787384559977363	6.8566586224048045
3	1.6438144727703032	3.5314923640918887
4	0.7408741285725311	2.1222113737265813
5	0.5402207187508039	1.9343072416778733
6	0.2906901962801945	1.2490098189119982
7	0.2572479613099066	1.289538161118582
8	0.19550845059552904	1.12005600280014
9	0.10289918452396266	0.6631910542895566
>10	0.9158027422632676	12.32061603080154
>50	0.06431199032747666	3.146473113129341
>100	0.010289918452396265	0.9929443840613084
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	178	0.3279111323987252	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	130	0.239485658493451	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	123	0.22659027688226516	TruSeq Adapter, Index 11 (95% over 21bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	108	0.19895731628686697	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	95	0.17500875043752187	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	93	0.17132435569146878	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	92	0.16948215831844224	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	87	0.16027117145330952	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	86	0.15842897408028297	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	79	0.14553359246909714	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	77	0.14184919772304405	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	75	0.13816480297699094	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	72	0.13263821085791133	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	71	0.1307960134848848	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	71	0.1307960134848848	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	66	0.12158502661975205	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	65	0.1197428292467255	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	64	0.11790063187369895	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	63	0.11605843450067241	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	59	0.10868964500856623	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	58	0.10684744763553967	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	57	0.10500525026251313	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	56	0.10316305288948657	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	56	0.10316305288948657	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	55	0.10132085551646003	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	55	0.10132085551646003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0018421973730265461	0.0
21	0.0	0.0	0.0	0.0018421973730265461	0.0
22	0.0	0.0	0.0	0.0036843947460530923	0.0
23	0.0	0.0	0.0	0.0036843947460530923	0.0
24	0.0	0.0	0.0	0.0073687894921061845	0.0
25	0.0	0.0	0.0	0.011053184238159276	0.0
26	0.0	0.0	0.0	0.016579776357238913	0.0
27	0.0	0.0	0.0	0.03684394746053092	0.0
28	0.0	0.0	0.0	0.17869314518357496	0.0
29	0.0	0.0	0.0	0.42186319842307907	0.0
30	0.0	0.0	0.0	0.7110881859882467	0.0
31	0.0	0.0	0.0	1.4313873588416264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	0.0018265225	37.0	28
TTCGTCC	20	0.0018265225	37.0	4
CCGCCTT	20	0.0018265225	37.0	17
CACCGGT	20	0.0018265225	37.0	27
GTATTCC	20	0.0018265225	37.0	32
CCACCGG	20	0.0018265225	37.0	26
GGTATCA	65	1.8189894E-12	34.153847	1
ATCTAAG	30	3.554857E-4	30.833334	32
CATCTAA	30	3.554857E-4	30.833334	31
CTAAGTC	30	3.554857E-4	30.833334	34
ACATCTA	30	3.554857E-4	30.833334	30
GTATTAG	25	0.0054508755	29.599998	1
TATTCCT	25	0.0054508755	29.599998	33
TCGTCCA	25	0.0054508755	29.599998	5
ATTCCTG	25	0.0054508755	29.599998	34
ACTGAAA	35	8.760481E-4	26.42857	24
TAAGTCT	35	8.760481E-4	26.42857	35
GCGAACC	35	8.760481E-4	26.42857	11
TCTAAGT	35	8.760481E-4	26.42857	33
TTATACA	50	8.884466E-6	25.899998	37
>>END_MODULE