FastQCFastQC Report
Fri 10 Feb 2017
ERR1632488.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632488.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84449
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1410.16696467690558797No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1080.12788783762981207No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1030.12196710440620967No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1010.1195988111167687No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA910.10775734466956388No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA910.10775734466956388No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT870.10302075809068197No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA860.10183661144596147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCCT200.001832291337.07
CTCTTTT250.00546789129.614
GGTAACG400.0019164823.12525
TTATACA555.089037E-420.18181837
GTATCAA1403.6379788E-1219.821431
CTTATAC1152.93403E-919.30434637
TCTTATA2350.018.89361837
GATACCA852.6750482E-517.4117642
GGATACC852.6750482E-517.4117641
GGTATCA852.6750482E-517.4117641
TCGAGCT904.367973E-516.44444527
CGCCCTA904.367973E-516.44444516
ATCGAGC904.367973E-516.44444526
GCTCACA803.3363572E-416.187531
AACTCCG700.002565907315.8571435
CCTACTC956.9366295E-515.57894819
ACGTGTC956.9366295E-515.5789488
CGAGCTC956.9366295E-515.57894828
TACTCAT956.9366295E-515.57894821
ACCACGT956.9366295E-515.5789485