##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632488.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	84449
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.055690416701204	34.0	33.0	34.0	31.0	34.0
2	33.20536655259387	34.0	33.0	34.0	31.0	34.0
3	33.28442018259541	34.0	34.0	34.0	31.0	34.0
4	36.563582754088266	37.0	37.0	37.0	35.0	37.0
5	36.53339885611434	37.0	37.0	37.0	35.0	37.0
6	36.5712915487454	37.0	37.0	37.0	35.0	37.0
7	36.56959821904345	37.0	37.0	37.0	35.0	37.0
8	36.53927222347215	37.0	37.0	37.0	35.0	37.0
9	38.32138924084359	39.0	39.0	39.0	37.0	39.0
10	38.368151191843594	39.0	39.0	39.0	37.0	39.0
11	38.42220748617509	39.0	39.0	39.0	37.0	39.0
12	38.39353929590641	39.0	39.0	39.0	37.0	39.0
13	38.4340015867565	39.0	39.0	39.0	37.0	39.0
14	39.99505026702507	41.0	40.0	41.0	38.0	41.0
15	39.9805089462279	41.0	40.0	41.0	38.0	41.0
16	39.952669658610525	41.0	40.0	41.0	38.0	41.0
17	39.95603263508153	41.0	40.0	41.0	38.0	41.0
18	39.9472699499106	41.0	40.0	41.0	38.0	41.0
19	39.94822910869282	41.0	40.0	41.0	38.0	41.0
20	39.923125199824746	41.0	40.0	41.0	38.0	41.0
21	39.89439780222383	41.0	40.0	41.0	38.0	41.0
22	39.86876102736563	41.0	40.0	41.0	38.0	41.0
23	39.82993285888524	41.0	40.0	41.0	38.0	41.0
24	39.82225958862745	41.0	40.0	41.0	38.0	41.0
25	39.77707255266492	41.0	40.0	41.0	38.0	41.0
26	39.70116875273834	41.0	40.0	41.0	38.0	41.0
27	39.63421710144584	41.0	40.0	41.0	37.0	41.0
28	39.59446529858258	41.0	40.0	41.0	37.0	41.0
29	39.55303200748381	41.0	40.0	41.0	37.0	41.0
30	39.50037300619309	41.0	40.0	41.0	37.0	41.0
31	39.451905884024676	41.0	40.0	41.0	37.0	41.0
32	39.400561285509596	41.0	39.0	41.0	36.0	41.0
33	39.34691944250376	41.0	39.0	41.0	36.0	41.0
34	39.30174424800767	41.0	39.0	41.0	36.0	41.0
35	39.228267948702765	41.0	39.0	41.0	35.0	41.0
36	39.18793591398359	41.0	39.0	41.0	35.0	41.0
37	39.15576264964653	41.0	39.0	41.0	35.0	41.0
38	39.07533540953712	41.0	39.0	41.0	35.0	41.0
39	39.00489052564269	41.0	39.0	41.0	35.0	41.0
40	38.91772549112482	40.0	39.0	41.0	35.0	41.0
41	38.87130694265178	40.0	39.0	41.0	35.0	41.0
42	38.80656964558491	40.0	38.0	41.0	35.0	41.0
43	38.10535352698078	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	1.0
20	6.0
21	11.0
22	11.0
23	28.0
24	40.0
25	78.0
26	98.0
27	123.0
28	190.0
29	266.0
30	341.0
31	460.0
32	613.0
33	789.0
34	1291.0
35	1935.0
36	3185.0
37	5839.0
38	15705.0
39	53437.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.889791471775865	16.72488721003209	12.47380075548556	27.91152056270649
2	21.398714016743835	18.6621511207948	31.886700849033144	28.052434013428222
3	21.062416369643216	19.540787931177398	28.5888524434866	30.807943255692784
4	16.06176508898862	15.164181932290493	33.08268896020083	35.691364018520055
5	17.282620279695436	32.709682767113875	32.89085720375611	17.116839749434572
6	36.040687278712596	32.90033037691388	14.604080569337707	16.45490177503582
7	30.935831093322598	26.854077608971096	19.613020876505345	22.597070421200964
8	27.899679096259284	30.822153015429432	19.270802496181126	22.00736539213016
9	27.891390069746237	12.67392153844332	17.282620279695436	42.152068112115
10	19.384480574074292	23.289796208362443	29.804971047614536	27.520752169948725
11	39.81456263543677	19.010290234342623	18.499923030468093	22.675224099752516
12	23.397553553032008	23.264929128823315	26.362656751412096	26.974860566732584
13	35.20941633411881	17.209203187722768	20.27732714419354	27.304053333964877
14	24.428945280583548	19.063576833355043	22.629042380608414	33.878435505453
15	29.468673400513918	24.061859820720198	19.339483001574916	27.12998377719097
16	26.267925019834454	23.713720707172374	22.20274958850904	27.815604684484125
17	27.008016672784755	22.986654667314	21.95289464647302	28.052434013428222
18	27.01156911271892	21.33595424457365	23.115726651588535	28.5367499911189
19	28.32597188835865	21.76224703667302	22.781797297777356	27.12998377719097
20	29.180925765846844	21.112150528721475	22.34366303923078	27.3632606662009
21	27.920993735864247	22.042889791471776	21.58462503996495	28.45149143269903
22	28.575826830394675	22.63614726047674	20.93097609207924	27.857049817049344
23	28.395836540397163	21.872372674632025	21.868820234697864	27.862970550272948
24	27.97428033487667	21.83566412864569	21.590545773188552	28.59950976328909
25	28.21584625039965	21.896055607526435	22.3969496382432	27.491148503830715
26	28.035855960402134	22.360241092256867	22.065388577721464	27.53851436961953
27	27.50417411692264	21.800139729304078	23.398737699676726	27.296948454096555
28	26.877760541865502	22.39458134495376	21.9978922189724	28.729765894208338
29	27.767054672050584	22.777060711198473	21.828559248777367	27.627325367973572
30	27.11577401745432	21.964736112920225	23.100332745207165	27.819157124418286
31	27.694821726722633	22.232353254627053	22.163672749233264	27.909152269417042
32	25.859394427405892	21.95289464647302	22.792454617579843	29.39525630854125
33	26.163720115099053	21.721986050752527	24.12225129960094	27.99204253454748
34	26.623169013250603	21.447264029177372	24.018046394865543	27.91152056270649
35	25.9233383462208	22.61483262087177	24.228824497625787	27.23300453528165
36	25.89965541332639	21.468578668782342	24.89786735189286	27.73389856599841
37	25.95767859891769	20.644412604056885	25.12285521438975	28.275053582635675
38	25.017466163009626	20.289168610640743	26.629089746474204	28.064275479875427
39	24.698930715579817	19.461450105981122	27.595353408566115	28.24426576987294
40	24.176722045258085	19.9860270695923	28.986725716112684	26.85052516903693
41	22.83153145685562	19.444872052955038	29.46630510722448	28.257291382964866
42	21.409371336546318	20.251275918009686	30.612559059313902	27.72679368613009
43	20.45494914090161	20.15654418643205	30.59834929957726	28.79015737308908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.5
4	3.0
5	3.0
6	4.5
7	6.0
8	4.5
9	3.0
10	9.0
11	15.0
12	15.0
13	21.0
14	27.0
15	44.5
16	62.0
17	69.5
18	77.0
19	77.0
20	84.0
21	91.0
22	88.5
23	86.0
24	101.5
25	117.0
26	117.0
27	160.5
28	204.0
29	275.5
30	347.0
31	457.0
32	567.0
33	567.0
34	783.5
35	1000.0
36	1146.0
37	1292.0
38	1746.0
39	2200.0
40	2200.0
41	2519.5
42	2839.0
43	3369.5
44	3900.0
45	4730.0
46	5560.0
47	5560.0
48	6344.5
49	7129.0
50	7864.0
51	8599.0
52	8821.5
53	9044.0
54	9044.0
55	8605.5
56	8167.0
57	7943.0
58	7719.0
59	7286.0
60	6853.0
61	6853.0
62	6347.5
63	5842.0
64	4937.0
65	4032.0
66	3531.5
67	3031.0
68	3031.0
69	2599.0
70	2167.0
71	1794.0
72	1421.0
73	1131.5
74	842.0
75	842.0
76	676.5
77	511.0
78	406.5
79	302.0
80	246.5
81	191.0
82	191.0
83	145.5
84	100.0
85	71.0
86	42.0
87	38.0
88	34.0
89	34.0
90	25.0
91	16.0
92	11.5
93	7.0
94	5.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	84449.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.6491847150351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.0170428966493	66.29681819796564
2	5.218985143739148	7.68748001752537
3	1.69464274229854	3.744271690606164
4	0.8392822689562031	2.472498194176367
5	0.495208695092932	1.8235858328695427
6	0.32317190816129654	1.4280808535329015
7	0.24278088623062577	1.2516430034695496
8	0.18811499131776963	1.1083612594583714
9	0.123802173773233	0.8206136247912941
>10	0.8071258601839347	10.646662482681855
>50	0.04341115184256222	2.183566412864569
>100	0.006431281754453663	0.5364184300583784
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	141	0.16696467690558797	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	108	0.12788783762981207	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	103	0.12196710440620967	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	101	0.1195988111167687	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	91	0.10775734466956388	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	91	0.10775734466956388	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	87	0.10302075809068197	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	86	0.10183661144596147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0011841466447204821	0.0
15	0.0	0.0	0.0	0.0011841466447204821	0.0011841466447204821
16	0.0	0.0	0.0	0.0023682932894409643	0.0011841466447204821
17	0.0	0.0	0.0	0.004736586578881929	0.0011841466447204821
18	0.0	0.0	0.0	0.004736586578881929	0.0011841466447204821
19	0.0	0.0	0.0	0.004736586578881929	0.0011841466447204821
20	0.0	0.0	0.0	0.004736586578881929	0.0011841466447204821
21	0.0	0.0	0.0	0.004736586578881929	0.0011841466447204821
22	0.0	0.0	0.0	0.01065731980248434	0.0011841466447204821
23	0.0	0.0	0.0	0.01065731980248434	0.0011841466447204821
24	0.0	0.0	0.0	0.01657805302608675	0.0011841466447204821
25	0.0	0.0	0.0	0.017762199670807232	0.0011841466447204821
26	0.0	0.0	0.0	0.018946346315527714	0.0011841466447204821
27	0.0	0.0	0.0	0.060391478880744594	0.0011841466447204821
28	0.0	0.0	0.0	0.18827931651055665	0.0011841466447204821
29	0.0	0.0	0.0	0.4191879122310507	0.0011841466447204821
30	0.0	0.0	0.0	0.6808843207142773	0.0011841466447204821
31	0.0	0.0	0.0	1.3499271749813497	0.0011841466447204821
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCCT	20	0.0018322913	37.0	7
CTCTTTT	25	0.005467891	29.6	14
GGTAACG	40	0.00191648	23.125	25
TTATACA	55	5.089037E-4	20.181818	37
GTATCAA	140	3.6379788E-12	19.82143	1
CTTATAC	115	2.93403E-9	19.304346	37
TCTTATA	235	0.0	18.893618	37
GATACCA	85	2.6750482E-5	17.411764	2
GGATACC	85	2.6750482E-5	17.411764	1
GGTATCA	85	2.6750482E-5	17.411764	1
TCGAGCT	90	4.367973E-5	16.444445	27
CGCCCTA	90	4.367973E-5	16.444445	16
ATCGAGC	90	4.367973E-5	16.444445	26
GCTCACA	80	3.3363572E-4	16.1875	31
AACTCCG	70	0.0025659073	15.857143	5
CCTACTC	95	6.9366295E-5	15.578948	19
ACGTGTC	95	6.9366295E-5	15.578948	8
CGAGCTC	95	6.9366295E-5	15.578948	28
TACTCAT	95	6.9366295E-5	15.578948	21
ACCACGT	95	6.9366295E-5	15.578948	5
>>END_MODULE