ERR1632487.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632487.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15461
Sequences flagged as poor quality	0
Sequence length	43
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	40	0.25871547765345065	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	33	0.21344026906409677	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	31	0.2005044951814242	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	30	0.19403660824008795	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	27	0.17463294741607915	TruSeq Adapter, Index 1 (95% over 22bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	26	0.1681650604747429	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	23	0.14876139965073412	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	21	0.13582562576806156	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	21	0.13582562576806156	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	20	0.12935773882672533	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	20	0.12935773882672533	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	20	0.12935773882672533	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	19	0.12288985188538903	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	19	0.12288985188538903	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	19	0.12288985188538903	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	18	0.11642196494405278	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	18	0.11642196494405278	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	17	0.1099540780027165	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	16	0.10348619106138024	No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA	16	0.10348619106138024	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	16	0.10348619106138024	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	16	0.10348619106138024	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	16	0.10348619106138024	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	80	6.093833E-7	20.8125	5
ACGCAGA	80	6.093833E-7	20.8125	6
TCAACGC	80	6.093833E-7	20.8125	3
CAGAGTA	80	6.093833E-7	20.8125	9
AGTACGG	80	6.093833E-7	20.8125	12
AGAGTAC	80	6.093833E-7	20.8125	10
GAGTACG	80	6.093833E-7	20.8125	11
CGCAGAG	80	6.093833E-7	20.8125	7
TATCAAC	80	6.093833E-7	20.8125	1
ATCAACG	80	6.093833E-7	20.8125	2
CAACGCA	80	6.093833E-7	20.8125	4
TCTCTTA	45	0.003660118	20.555557	37
GCAGAGT	90	1.8871615E-6	18.5	8
GTACGGG	95	3.1645995E-6	17.526316	13
GTCTCTT	80	3.120629E-4	16.1875	37
TGTCTCT	120	4.634656E-4	12.333333	37
CTGTCTC	160	0.0051146126	9.25	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content