##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632487.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15461
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02645365759007	34.0	33.0	34.0	31.0	34.0
2	33.17366276437488	34.0	33.0	34.0	31.0	34.0
3	33.23006273850333	34.0	34.0	34.0	31.0	34.0
4	36.52428691546472	37.0	37.0	37.0	35.0	37.0
5	36.50585343768191	37.0	37.0	37.0	35.0	37.0
6	36.53075480240605	37.0	37.0	37.0	35.0	37.0
7	36.532759847357866	37.0	37.0	37.0	35.0	37.0
8	36.511480499320875	37.0	37.0	37.0	35.0	37.0
9	38.304572796067525	39.0	39.0	39.0	37.0	39.0
10	38.328956729836364	39.0	39.0	39.0	37.0	39.0
11	38.40243192548994	39.0	39.0	39.0	37.0	39.0
12	38.39027229804023	39.0	39.0	39.0	37.0	39.0
13	38.3983571567169	39.0	39.0	39.0	37.0	39.0
14	39.98531789664317	41.0	40.0	41.0	38.0	41.0
15	39.9627449712179	41.0	40.0	41.0	38.0	41.0
16	39.944311493435094	41.0	40.0	41.0	38.0	41.0
17	39.93713213893021	41.0	40.0	41.0	38.0	41.0
18	39.882931246361814	41.0	40.0	41.0	38.0	41.0
19	39.89327986546795	41.0	40.0	41.0	38.0	41.0
20	39.849104197658626	41.0	40.0	41.0	38.0	41.0
21	39.8557661212082	41.0	40.0	41.0	38.0	41.0
22	39.802212017333936	41.0	40.0	41.0	38.0	41.0
23	39.77420606687795	41.0	40.0	41.0	38.0	41.0
24	39.73242351723692	41.0	40.0	41.0	38.0	41.0
25	39.707392794773945	41.0	40.0	41.0	37.0	41.0
26	39.62447448418602	41.0	40.0	41.0	37.0	41.0
27	39.543108466464005	41.0	40.0	41.0	37.0	41.0
28	39.47894702800595	41.0	40.0	41.0	37.0	41.0
29	39.45249337041589	41.0	40.0	41.0	36.0	41.0
30	39.4201539357092	41.0	39.0	41.0	36.0	41.0
31	39.33173792122113	41.0	39.0	41.0	36.0	41.0
32	39.31983700924908	41.0	39.0	41.0	36.0	41.0
33	39.26311364077356	41.0	39.0	41.0	35.0	41.0
34	39.20108660500615	41.0	39.0	41.0	35.0	41.0
35	39.11726279024643	41.0	39.0	41.0	35.0	41.0
36	39.13129810490913	41.0	39.0	41.0	35.0	41.0
37	39.02095595368993	41.0	39.0	41.0	35.0	41.0
38	38.90311105361878	40.0	39.0	41.0	35.0	41.0
39	38.845417502102066	40.0	38.0	41.0	35.0	41.0
40	38.72705517107561	40.0	38.0	41.0	35.0	41.0
41	38.668068042170624	40.0	38.0	41.0	35.0	41.0
42	38.597697432248886	40.0	38.0	41.0	35.0	41.0
43	37.919474807580364	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	1.0
22	6.0
23	3.0
24	6.0
25	15.0
26	14.0
27	32.0
28	48.0
29	50.0
30	67.0
31	100.0
32	121.0
33	151.0
34	245.0
35	416.0
36	646.0
37	1080.0
38	2911.0
39	9545.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5353470021344	15.626414850268416	12.269581527714896	28.568656619882283
2	21.783843218420543	18.452881443632364	31.56975616066231	28.19351917728478
3	21.499256193001745	19.332514067654095	28.115904533988743	31.05232520535541
4	16.45430437875946	13.802470732811589	32.72104003622017	37.02218485220878
5	17.831964297264083	32.19067330703059	32.68223271457215	17.295129681133172
6	36.97690964361943	32.70810426233749	14.00944311493435	16.305542979108726
7	32.772783131750856	25.47700666192355	18.860358320936548	22.889851885389042
8	29.021408705775826	29.461225017786692	19.410128710950133	22.107237565487356
9	28.141776081754088	13.233296681974	16.493111700407475	42.13181553586443
10	20.600219908156006	22.230127417372746	28.7497574542397	28.41989522023155
11	39.88745876722075	18.698661147403143	18.032468792445506	23.381411292930597
12	24.97251148049932	22.036090809132656	24.59737403790182	28.394023672466208
13	34.72608498803441	16.816506047474288	19.869348683785006	28.588060280706294
14	24.222236595304313	18.536963973869735	22.327145721492787	34.91365370933316
15	30.664251988875236	22.488842895026195	19.371321389302114	27.475583726796454
16	27.326822327145724	22.63760429467693	20.54200892568398	29.49356445249337
17	27.559666257033825	23.01920962421577	20.813660177220104	28.607463941530302
18	26.473061250889334	20.64549511674536	22.94159498091973	29.939848651445573
19	29.635857965202767	21.30521958476166	21.66742125347649	27.39150119655908
20	28.969665610245134	20.360908091326564	22.327145721492787	28.342280576935515
21	29.54530754802406	20.580816247331995	21.0271004462842	28.846775758359744
22	29.241316861781257	21.92613673112994	20.121596274497122	28.710950132591684
23	29.829894573442857	21.550999288532434	20.496733717094624	28.12237242093008
24	29.713472608498805	20.72957764698273	20.66489877756937	28.892050966949096
25	29.073151801306512	20.412651186857254	22.107237565487356	28.40695944634888
26	29.312463618135954	21.156458185110925	21.27934803699631	28.251730159756804
27	29.50650022637604	21.014164672401524	21.738568009831187	27.740767091391245
28	27.22980402302568	21.738568009831187	22.243063191255416	28.788564775887714
29	29.144298557661212	22.074898130780674	21.369898454175022	27.410904857383095
30	27.48205161373779	20.380311752150572	23.55604424034668	28.58159239376496
31	27.86365694327663	21.247008602289633	21.81618265312722	29.073151801306512
32	26.33076773817994	21.434577323588382	22.624668520794255	29.609986417437423
33	29.157234331543886	20.97535735075351	22.314209947610117	27.553198370092492
34	27.333290214087057	21.22760494146562	23.232649893279866	28.206454951167455
35	27.475583726796454	22.94159498091973	22.4176961386715	27.165125153612312
36	27.960675247396676	20.742513420865404	23.11622792833581	28.18058340340211
37	26.647694198305416	21.201733393700277	22.94159498091973	29.208977427074572
38	27.39150119655908	20.043981631201085	24.80434642002458	27.760170752215252
39	25.25709850591812	20.225082465558504	25.489942435806224	29.02787659271716
40	25.716318478752992	20.2962292219132	27.055171075609596	26.932281223724207
41	23.284392988810556	20.08925683979044	27.132785718905634	29.49356445249337
42	22.469439234202184	21.11118297652157	28.258198046698148	28.161179742578103
43	22.792833581268997	19.41659659789147	28.79503266282906	28.995537158010475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	0.5
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	3.0
15	8.0
16	13.0
17	10.0
18	7.0
19	7.0
20	8.5
21	10.0
22	9.0
23	8.0
24	12.0
25	16.0
26	16.0
27	20.0
28	24.0
29	36.0
30	48.0
31	55.5
32	63.0
33	63.0
34	105.0
35	147.0
36	172.5
37	198.0
38	243.5
39	289.0
40	289.0
41	364.0
42	439.0
43	535.5
44	632.0
45	760.5
46	889.0
47	889.0
48	997.0
49	1105.0
50	1282.5
51	1460.0
52	1592.5
53	1725.0
54	1725.0
55	1680.0
56	1635.0
57	1556.0
58	1477.0
59	1310.5
60	1144.0
61	1144.0
62	1162.0
63	1180.0
64	996.5
65	813.0
66	686.5
67	560.0
68	560.0
69	477.5
70	395.0
71	371.5
72	348.0
73	344.0
74	340.0
75	340.0
76	336.0
77	332.0
78	217.5
79	103.0
80	63.5
81	24.0
82	24.0
83	19.5
84	15.0
85	12.0
86	9.0
87	6.5
88	4.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	15461.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.12088480693357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.81302291742452	61.030981178449
2	6.784285194616224	9.650087316473709
3	2.646416878865042	5.64646529978656
4	1.4368861404146962	4.08770454692452
5	0.8548563113859585	3.0399068624280448
6	0.6365951255001819	2.7165125153612317
7	0.42742815569297926	2.127934803699631
8	0.29101491451436884	1.6557790569820838
9	0.2819206984357948	1.804540456632818
>10	0.8275736631502365	8.240087963262402
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT	40	0.25871547765345065	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT	33	0.21344026906409677	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	31	0.2005044951814242	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	30	0.19403660824008795	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	27	0.17463294741607915	TruSeq Adapter, Index 1 (95% over 22bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	26	0.1681650604747429	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	23	0.14876139965073412	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	21	0.13582562576806156	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	21	0.13582562576806156	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG	20	0.12935773882672533	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	20	0.12935773882672533	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	20	0.12935773882672533	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	19	0.12288985188538903	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	19	0.12288985188538903	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	19	0.12288985188538903	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	18	0.11642196494405278	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	18	0.11642196494405278	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	17	0.1099540780027165	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	16	0.10348619106138024	No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA	16	0.10348619106138024	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	16	0.10348619106138024	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	16	0.10348619106138024	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	16	0.10348619106138024	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.006467886941336266	0.0
21	0.0	0.0	0.0	0.006467886941336266	0.0
22	0.0	0.0	0.0	0.006467886941336266	0.0
23	0.0	0.0	0.0	0.006467886941336266	0.0
24	0.0	0.0	0.0	0.006467886941336266	0.0
25	0.0	0.0	0.0	0.006467886941336266	0.0
26	0.0	0.0	0.0	0.019403660824008795	0.0
27	0.0	0.0	0.0	0.025871547765345063	0.0
28	0.0	0.0	0.0	0.07761464329603518	0.0
29	0.0	0.0	0.0	0.2910549123601319	0.0
30	0.0	0.0	0.0	0.5174309553069012	0.0
31	0.0	0.0	0.0	0.9895867020244487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGCAG	80	6.093833E-7	20.8125	5
ACGCAGA	80	6.093833E-7	20.8125	6
TCAACGC	80	6.093833E-7	20.8125	3
CAGAGTA	80	6.093833E-7	20.8125	9
AGTACGG	80	6.093833E-7	20.8125	12
AGAGTAC	80	6.093833E-7	20.8125	10
GAGTACG	80	6.093833E-7	20.8125	11
CGCAGAG	80	6.093833E-7	20.8125	7
TATCAAC	80	6.093833E-7	20.8125	1
ATCAACG	80	6.093833E-7	20.8125	2
CAACGCA	80	6.093833E-7	20.8125	4
TCTCTTA	45	0.003660118	20.555557	37
GCAGAGT	90	1.8871615E-6	18.5	8
GTACGGG	95	3.1645995E-6	17.526316	13
GTCTCTT	80	3.120629E-4	16.1875	37
TGTCTCT	120	4.634656E-4	12.333333	37
CTGTCTC	160	0.0051146126	9.25	36
>>END_MODULE