FastQCFastQC Report
Fri 10 Feb 2017
ERR1632486.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632486.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791224
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA20710.26174635754223835No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA14940.18882137043365724No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA12620.15949971183887243No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT11540.14584997421716228No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT11400.14408056378471837No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11280.14256392627119502No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10290.13005166678462735No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT9880.1248698219467559No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT9320.11779218021698028No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA9070.11463251873047328No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT8670.10957706035206213No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8560.10818680929799904No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8540.10793403637907849No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8380.10591185302771403No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8000.10110916756822341No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC7950.10047723527092202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT250.005495322429.59999810
GGTATCA9950.028.6331651
ATACGGC1002.0008883E-1124.0529
CTAAGTC952.8012437E-1023.36842234
CTCTTAA400.001930658123.1251
CATGTTA502.701072E-422.19999925
ATCTTAC855.1755705E-821.7647061
TAAGTCT1059.822543E-1021.14285735
CTTACTT705.098609E-621.14285733
GTTTAAT450.003824769320.5555571
ACTTATA450.003824769320.55555731
CGCTCTT450.003824769320.55555727
TATTATG450.003824769320.55555723
GTATCAA14000.020.2178572
TTCTAGC555.141148E-420.1818182
TACGGCT1303.274181E-1119.92307730
TATGAGT656.898655E-519.9230774
TTTGGTA759.259789E-619.73333432
GCGGTAA8200.019.6280523
GACCGTT851.2443052E-619.5882367
Produced by FastQC (version 0.11.5)