##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632485.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.88296112489661	34.0	33.0	34.0	31.0	34.0
2	33.02564102564103	34.0	33.0	34.0	31.0	34.0
3	33.10421836228288	34.0	33.0	34.0	31.0	34.0
4	36.36393713813069	37.0	37.0	37.0	35.0	37.0
5	36.37179487179487	37.0	37.0	37.0	35.0	37.0
6	36.42803970223325	37.0	37.0	37.0	35.0	37.0
7	36.42307692307692	37.0	37.0	37.0	35.0	37.0
8	36.383374689826304	37.0	37.0	37.0	35.0	37.0
9	38.102977667493796	39.0	39.0	39.0	37.0	39.0
10	38.20967741935484	39.0	39.0	39.0	37.0	39.0
11	38.22249793217535	39.0	39.0	39.0	37.0	39.0
12	38.27874276261373	39.0	39.0	39.0	37.0	39.0
13	38.28618693134822	39.0	39.0	39.0	37.0	39.0
14	39.812655086848636	41.0	40.0	41.0	38.0	41.0
15	39.87882547559967	41.0	40.0	41.0	38.0	41.0
16	39.76923076923077	41.0	40.0	41.0	38.0	41.0
17	39.76220016542597	41.0	40.0	41.0	38.0	41.0
18	39.774193548387096	41.0	40.0	41.0	38.0	41.0
19	39.75186104218362	41.0	40.0	41.0	38.0	41.0
20	39.766335814722915	41.0	40.0	41.0	38.0	41.0
21	39.72043010752688	41.0	40.0	41.0	37.0	41.0
22	39.68279569892473	41.0	40.0	41.0	37.0	41.0
23	39.626550868486355	41.0	40.0	41.0	37.0	41.0
24	39.60711331679074	41.0	40.0	41.0	37.0	41.0
25	39.60669975186104	41.0	40.0	41.0	37.0	41.0
26	39.5	41.0	40.0	41.0	37.0	41.0
27	39.44251447477254	41.0	39.0	41.0	36.0	41.0
28	39.310587262200166	41.0	39.0	41.0	36.0	41.0
29	39.25062034739454	41.0	39.0	41.0	36.0	41.0
30	39.29942100909843	41.0	39.0	41.0	36.0	41.0
31	39.146401985111666	41.0	39.0	41.0	35.0	41.0
32	39.07402812241522	41.0	39.0	41.0	35.0	41.0
33	38.94210090984284	40.0	39.0	41.0	35.0	41.0
34	39.12406947890819	41.0	39.0	41.0	35.0	41.0
35	39.005376344086024	40.0	39.0	41.0	35.0	41.0
36	38.91645988420182	40.0	39.0	41.0	35.0	41.0
37	38.87179487179487	40.0	39.0	41.0	35.0	41.0
38	38.70388751033912	40.0	38.0	41.0	35.0	41.0
39	38.59181141439206	40.0	38.0	41.0	35.0	41.0
40	38.49214226633581	40.0	38.0	41.0	35.0	41.0
41	38.4317617866005	40.0	38.0	41.0	35.0	41.0
42	38.337882547559964	40.0	38.0	41.0	34.0	41.0
43	37.758478081058726	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
25	3.0
26	5.0
27	7.0
28	4.0
29	11.0
30	18.0
31	18.0
32	29.0
33	36.0
34	57.0
35	66.0
36	101.0
37	176.0
38	501.0
39	1386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3904052936311	16.74937965260546	12.531017369727046	28.329197684036394
2	22.208436724565754	19.396195202646815	29.56989247311828	28.825475599669147
3	21.7535153019024	18.693134822167078	27.377998345740277	32.17535153019024
4	16.21174524400331	14.350703060380479	32.5889164598842	36.848635235732004
5	16.04631927212572	33.37468982630273	32.50620347394541	18.07278742762614
6	35.8560794044665	33.126550868486355	14.061207609594707	16.95616211745244
7	29.900744416873447	27.626137303556657	18.941273779983458	23.531844499586434
8	29.114971050454923	28.618693134822166	19.230769230769234	23.03556658395368
9	27.874276261373037	12.076095947063688	16.708023159636063	43.34160463192721
10	21.7535153019024	21.21588089330025	27.791563275434246	29.23904052936311
11	40.90157154673283	18.11414392059553	16.832092638544253	24.152191894127377
12	22.497932175351533	24.731182795698924	24.937965260545905	27.83291976840364
13	34.946236559139784	17.94871794871795	20.306038047973534	26.799007444168733
14	24.85525227460711	20.926385442514476	21.257237386269644	32.961124896608766
15	29.114971050454923	23.283705541770058	19.065343258891644	28.53598014888337
16	26.01323407775021	22.167080231596362	22.994210090984286	28.825475599669147
17	28.701406120760957	23.449131513647643	19.809760132340777	28.03970223325062
18	25.88916459884202	19.065343258891644	22.8287841191067	32.21670802315963
19	29.69396195202647	20.926385442514476	21.464019851116625	27.915632754342433
20	30.893300248138956	20.885028949545077	20.760959470636887	27.46071133167907
21	29.114971050454923	20.6782464846981	21.960297766749378	28.2464846980976
22	28.66004962779156	20.306038047973534	20.802315963606286	30.23159636062862
23	29.69396195202647	20.388751033912325	20.967741935483872	28.949545078577337
24	27.915632754342433	21.42266335814723	21.339950372208435	29.3217535153019
25	28.577336641852767	22.41521918941274	22.58064516129032	26.426799007444167
26	28.66004962779156	21.960297766749378	21.83622828784119	27.543424317617866
27	27.58478081058726	22.125723738626967	22.58064516129032	27.70885028949545
28	26.550868486352357	21.7535153019024	19.933829611248967	31.76178660049628
29	27.750206782464847	22.41521918941274	21.62944582299421	28.205128205128204
30	28.329197684036394	21.54673283705542	23.159636062861868	26.96443341604632
31	27.29528535980149	22.001654259718777	22.043010752688172	28.66004962779156
32	25.392886683209266	20.6368899917287	23.449131513647643	30.52109181141439
33	25.599669148056243	21.381306865177834	24.607113316790734	28.41191066997519
34	26.716294458229946	20.719602977667495	23.49048800661704	29.073614557485527
35	24.9793217535153	23.449131513647643	24.11083540115798	27.46071133167907
36	26.799007444168733	23.076923076923077	22.994210090984286	27.129859387923904
37	26.50951199338296	20.760959470636887	24.152191894127377	28.577336641852767
38	27.874276261373037	21.174524400330853	22.91149710504549	28.03970223325062
39	25.971877584780813	20.967741935483872	26.50951199338296	26.550868486352357
40	25.351530190239867	20.926385442514476	27.2125723738627	26.50951199338296
41	23.69727047146402	17.907361455748553	29.073614557485527	29.3217535153019
42	21.7535153019024	20.967741935483872	29.48717948717949	27.791563275434246
43	22.29114971050455	21.257237386269644	27.750206782464847	28.701406120760957
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	1.0
15	1.5
16	2.0
17	3.0
18	4.0
19	4.0
20	4.5
21	5.0
22	4.5
23	4.0
24	3.5
25	3.0
26	3.0
27	2.5
28	2.0
29	6.0
30	10.0
31	10.5
32	11.0
33	11.0
34	16.5
35	22.0
36	22.5
37	23.0
38	31.0
39	39.0
40	39.0
41	44.5
42	50.0
43	64.5
44	79.0
45	105.0
46	131.0
47	131.0
48	170.0
49	209.0
50	218.5
51	228.0
52	259.5
53	291.0
54	291.0
55	268.0
56	245.0
57	242.5
58	240.0
59	228.5
60	217.0
61	217.0
62	215.0
63	213.0
64	176.0
65	139.0
66	116.5
67	94.0
68	94.0
69	77.0
70	60.0
71	52.5
72	45.0
73	33.0
74	21.0
75	21.0
76	16.5
77	12.0
78	9.0
79	6.0
80	6.5
81	7.0
82	7.0
83	4.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2418.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.62696443341605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.34717685487635	81.84449958643508
2	5.506299580027998	9.760132340777503
3	1.1665888940737283	3.101736972704715
4	0.3266448903406439	1.1579818031430935
5	0.23331777881474566	1.0339123242349049
6	0.18665422305179655	0.9925558312655087
7	0.09332711152589827	0.5789909015715468
8	0.04666355576294914	0.3308519437551696
9	0.0	0.0
>10	0.09332711152589827	1.1993382961124897
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	17	0.7030603804797354	TruSeq Adapter, Index 8 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	12	0.49627791563275436	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	8	0.3308519437551696	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	7	0.2894954507857734	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	7	0.2894954507857734	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	6	0.24813895781637718	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	6	0.24813895781637718	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	6	0.24813895781637718	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	6	0.24813895781637718	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	5	0.20678246484698098	No Hit
GTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCT	5	0.20678246484698098	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	5	0.20678246484698098	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	5	0.20678246484698098	No Hit
GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT	5	0.20678246484698098	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	4	0.1654259718775848	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	4	0.1654259718775848	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	4	0.1654259718775848	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT	4	0.1654259718775848	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	4	0.1654259718775848	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	4	0.1654259718775848	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	4	0.1654259718775848	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	3	0.12406947890818859	No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACCGGGGCTTC	3	0.12406947890818859	No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC	3	0.12406947890818859	No Hit
CACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGC	3	0.12406947890818859	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	3	0.12406947890818859	No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA	3	0.12406947890818859	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.12406947890818859	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	3	0.12406947890818859	RNA PCR Primer, Index 8 (95% over 23bp)
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	3	0.12406947890818859	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	3	0.12406947890818859	No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	3	0.12406947890818859	No Hit
ACGCTGGCCTTCTCGGGGTGGGGGTCCCCGCATGCTGCTGTCC	3	0.12406947890818859	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	3	0.12406947890818859	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	3	0.12406947890818859	No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG	3	0.12406947890818859	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC	3	0.12406947890818859	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	3	0.12406947890818859	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	3	0.12406947890818859	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	3	0.12406947890818859	No Hit
CCCCCACCCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC	3	0.12406947890818859	No Hit
ACACAGCACTGTGCAAACACGAAAGTGGACGTATACGGTGTGA	3	0.12406947890818859	No Hit
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	3	0.12406947890818859	No Hit
CATCAGGCTTGCGCCCATTGTGCAATATTCCCCACCTGTCTCT	3	0.12406947890818859	No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT	3	0.12406947890818859	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	3	0.12406947890818859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0413564929693962	0.0
28	0.0	0.0	0.0	0.0827129859387924	0.0
29	0.0	0.0	0.0	0.24813895781637718	0.0
30	0.0	0.0	0.0	0.37220843672456577	0.0
31	0.0	0.0	0.0	0.7857733664185277	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE