FastQCFastQC Report
Fri 10 Feb 2017
ERR1632484.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632484.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4921
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT330.6705954074375127TruSeq Adapter, Index 6 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT170.3454582401950823No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC170.3454582401950823No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA150.30481609428977846No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA130.2641739483844747No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC130.2641739483844747No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC120.24385287543182277No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC120.24385287543182277RNA PCR Primer, Index 6 (95% over 22bp)
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC110.2235318024791709No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT110.2235318024791709No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT100.203210729526519No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC100.203210729526519No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA100.203210729526519No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA90.1828896565738671No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA90.1828896565738671No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA90.1828896565738671No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA80.1625685836212152No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC80.1625685836212152No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA80.1625685836212152No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA80.1625685836212152No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC80.1625685836212152No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG80.1625685836212152No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA80.1625685836212152No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC70.1422475106685633No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG70.1422475106685633No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG70.1422475106685633No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC70.1422475106685633No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC70.1422475106685633No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT70.1422475106685633No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC70.1422475106685633No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC70.1422475106685633No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT70.1422475106685633No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT70.1422475106685633No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG70.1422475106685633No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA60.12192643771591138No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC60.12192643771591138No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT60.12192643771591138No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG60.12192643771591138No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC60.12192643771591138No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT60.12192643771591138No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA60.12192643771591138No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC60.12192643771591138No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG60.12192643771591138No Hit
GCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAAT60.12192643771591138No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC60.12192643771591138No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC60.12192643771591138No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA60.12192643771591138No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT60.12192643771591138No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA60.12192643771591138No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG50.1016053647632595No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC50.1016053647632595No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC50.1016053647632595No Hit
GTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACC50.1016053647632595No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT50.1016053647632595No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA50.1016053647632595No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA50.1016053647632595No Hit
GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGG50.1016053647632595No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT50.1016053647632595No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC50.1016053647632595No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA50.1016053647632595No Hit
TCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTG50.1016053647632595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCA200.001668855537.010
Produced by FastQC (version 0.11.5)