##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632484.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4921
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.98597845966267	34.0	33.0	34.0	31.0	34.0
2	33.13289981711034	34.0	33.0	34.0	31.0	34.0
3	33.21093273724853	34.0	33.0	34.0	31.0	34.0
4	36.508230034545825	37.0	37.0	37.0	35.0	37.0
5	36.47165210323105	37.0	37.0	37.0	35.0	37.0
6	36.51148140621825	37.0	37.0	37.0	35.0	37.0
7	36.51778093883357	37.0	37.0	37.0	35.0	37.0
8	36.497866287339974	37.0	37.0	37.0	35.0	37.0
9	38.23999187157082	39.0	39.0	39.0	37.0	39.0
10	38.31741515952042	39.0	39.0	39.0	37.0	39.0
11	38.352164194269456	39.0	39.0	39.0	37.0	39.0
12	38.27331843121317	39.0	39.0	39.0	37.0	39.0
13	38.33529770371876	39.0	39.0	39.0	37.0	39.0
14	39.88559235927657	41.0	40.0	41.0	38.0	41.0
15	39.90103637472058	41.0	40.0	41.0	38.0	41.0
16	39.89128225970331	41.0	40.0	41.0	38.0	41.0
17	39.86466165413534	41.0	40.0	41.0	38.0	41.0
18	39.862426336110545	41.0	40.0	41.0	38.0	41.0
19	39.830522251574884	41.0	40.0	41.0	38.0	41.0
20	39.85023369233895	41.0	40.0	41.0	38.0	41.0
21	39.80674659622028	41.0	40.0	41.0	38.0	41.0
22	39.714082503556185	41.0	40.0	41.0	38.0	41.0
23	39.703921967079864	41.0	40.0	41.0	38.0	41.0
24	39.70636049583418	41.0	40.0	41.0	37.0	41.0
25	39.66104450314977	41.0	40.0	41.0	37.0	41.0
26	39.523674049989836	41.0	40.0	41.0	37.0	41.0
27	39.47856126803495	41.0	40.0	41.0	37.0	41.0
28	39.443609022556394	41.0	40.0	41.0	37.0	41.0
29	39.40520219467588	41.0	39.0	41.0	37.0	41.0
30	39.36252794147531	41.0	39.0	41.0	36.0	41.0
31	39.296890875838244	41.0	39.0	41.0	36.0	41.0
32	39.3241211135948	41.0	39.0	41.0	36.0	41.0
33	39.160942897785006	41.0	39.0	41.0	35.0	41.0
34	39.11440764072343	41.0	39.0	41.0	35.0	41.0
35	39.0268238162975	41.0	39.0	41.0	35.0	41.0
36	38.96911196911197	40.0	39.0	41.0	35.0	41.0
37	38.98293029871977	40.0	39.0	41.0	35.0	41.0
38	38.87055476529161	40.0	39.0	41.0	35.0	41.0
39	38.74232879496037	40.0	38.0	41.0	35.0	41.0
40	38.69172932330827	40.0	38.0	41.0	35.0	41.0
41	38.63442389758179	40.0	38.0	41.0	35.0	41.0
42	38.52529973582605	40.0	38.0	41.0	35.0	41.0
43	37.945945945945944	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	1.0
21	0.0
22	0.0
23	1.0
24	2.0
25	5.0
26	9.0
27	8.0
28	14.0
29	25.0
30	29.0
31	29.0
32	38.0
33	48.0
34	92.0
35	118.0
36	184.0
37	393.0
38	961.0
39	2963.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.81650071123755	15.708189392399918	13.411908148750253	28.063401747612275
2	21.47937411095306	19.04084535663483	31.619589514326357	27.860191018085757
3	22.12964844543792	18.69538711643975	29.01849217638691	30.156472261735416
4	16.114610851452955	15.5049786628734	34.36293436293436	34.01747612273928
5	17.862223125381018	32.920138183296075	30.82706766917293	18.39057102214997
6	33.85490753911807	33.14366998577525	15.40337329811014	17.598049176996543
7	29.72972972972973	26.986384881121722	19.284698232066653	23.999187157081895
8	28.24629140418614	29.242023978866083	20.84942084942085	21.662263767526927
9	26.07193659825239	13.229018492176387	17.374517374517374	43.32452753505385
10	19.87400934769356	22.536069904490958	28.673033936191832	28.916886811623655
11	40.39829302987198	18.553139605771186	18.146718146718147	22.90184921763869
12	25.25909368014631	23.044096728307252	25.03556187766714	26.661247713879295
13	35.724446250762036	17.33387522861207	20.503962609225766	26.437715911400122
14	24.48689290794554	20.808778703515546	22.33285917496444	32.37146921357448
15	29.242023978866083	23.917902865271287	18.796992481203006	28.04308067465962
16	25.889046941678522	23.369233895549684	21.804511278195488	28.937207884576306
17	27.372485267222107	23.795976427555374	20.62588904694168	28.205649258280836
18	27.33184312131681	20.74781548465759	22.210932737248527	29.709408656777075
19	30.725462304409675	22.779922779922778	20.280430806746597	26.21418410892095
20	30.400325137167243	20.585246901036374	22.434464539727696	26.579963422068687
21	29.05913432229222	20.808778703515546	20.97134728713676	29.160739687055475
22	28.063401747612275	22.800243852875433	21.31680552733184	27.819548872180448
23	28.063401747612275	21.865474497053444	21.60130054866897	28.46982320666531
24	28.185328185328185	21.19487908961593	22.271895956106484	28.347896768949397
25	29.770371875635032	22.820564925828084	20.95102621418411	26.458036984352773
26	28.04308067465962	23.10505994716521	21.763869132290186	27.087990245884985
27	28.165007112375534	21.459053038000405	23.145702093070515	27.230237756553542
28	26.254826254826252	22.475106685633	21.824832351148142	29.445234708392604
29	27.433448486080064	23.20666531192847	21.62162162162162	27.738264580369844
30	27.94147530989636	21.946758788864052	23.125381020117864	26.986384881121722
31	28.388538914854706	21.804511278195488	21.804511278195488	28.00243852875432
32	26.53932127616338	21.641942694574272	21.337126600284495	30.48160942897785
33	25.665515139199353	21.967079861816703	24.121113594797805	28.24629140418614
34	26.640926640926644	22.149969518390574	23.491160333265597	27.717943507417193
35	26.092257671205036	22.88152814468604	24.5884982727088	26.437715911400122
36	25.462304409672832	21.946758788864052	23.97886608412924	28.612070717333875
37	26.80349522454786	20.910384068278805	23.795976427555374	28.49014427961796
38	26.397073765494817	20.158504369030688	25.848404795773217	27.59601706970128
39	26.092257671205036	19.183092867303394	25.909368014631173	28.815281446860396
40	24.994919731761836	20.84942084942085	27.51473277789067	26.640926640926644
41	23.917902865271287	19.894330420646213	28.165007112375534	28.02275960170697
42	20.869741922373503	21.19487908961593	31.091241617557408	26.844137370453158
43	20.422678317415162	21.133915870757978	29.221702905913432	29.221702905913432
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	2.0
13	1.5
14	1.0
15	2.5
16	4.0
17	3.0
18	2.0
19	2.0
20	3.5
21	5.0
22	5.0
23	5.0
24	6.0
25	7.0
26	7.0
27	6.0
28	5.0
29	9.5
30	14.0
31	20.0
32	26.0
33	26.0
34	31.5
35	37.0
36	42.0
37	47.0
38	72.5
39	98.0
40	98.0
41	104.0
42	110.0
43	132.5
44	155.0
45	256.0
46	357.0
47	357.0
48	388.0
49	419.0
50	493.0
51	567.0
52	581.0
53	595.0
54	595.0
55	576.0
56	557.0
57	538.5
58	520.0
59	470.5
60	421.0
61	421.0
62	358.5
63	296.0
64	255.0
65	214.0
66	188.0
67	162.0
68	162.0
69	145.5
70	129.0
71	104.5
72	80.0
73	54.0
74	28.0
75	28.0
76	24.5
77	21.0
78	16.5
79	12.0
80	12.0
81	12.0
82	12.0
83	8.5
84	5.0
85	4.0
86	3.0
87	2.5
88	2.0
89	2.0
90	1.0
91	0.0
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4921.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.83722820564925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.79386626445451	72.58687258687259
2	5.706385118149824	9.225767120503962
3	1.7848164906988435	4.328388538914854
4	1.1814982403217698	3.8203617150985574
5	0.301659125188537	1.2192643771591138
6	0.3770739064856712	1.8288965657386709
7	0.2765208647561589	1.5647226173541962
8	0.17596782302664657	1.1379800853485065
9	0.07541478129713425	0.5486689697216013
>10	0.32679738562091504	3.7390774232879496
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	33	0.6705954074375127	TruSeq Adapter, Index 6 (95% over 21bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	17	0.3454582401950823	No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	17	0.3454582401950823	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	15	0.30481609428977846	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	13	0.2641739483844747	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	13	0.2641739483844747	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	12	0.24385287543182277	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	12	0.24385287543182277	RNA PCR Primer, Index 6 (95% over 22bp)
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	11	0.2235318024791709	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	11	0.2235318024791709	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	10	0.203210729526519	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	10	0.203210729526519	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	10	0.203210729526519	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	9	0.1828896565738671	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	9	0.1828896565738671	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	9	0.1828896565738671	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	8	0.1625685836212152	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	8	0.1625685836212152	No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA	8	0.1625685836212152	No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA	8	0.1625685836212152	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	8	0.1625685836212152	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	8	0.1625685836212152	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	8	0.1625685836212152	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	7	0.1422475106685633	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	7	0.1422475106685633	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	7	0.1422475106685633	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	7	0.1422475106685633	No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	7	0.1422475106685633	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	7	0.1422475106685633	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	7	0.1422475106685633	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	7	0.1422475106685633	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	7	0.1422475106685633	No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT	7	0.1422475106685633	No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG	7	0.1422475106685633	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	6	0.12192643771591138	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	6	0.12192643771591138	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	6	0.12192643771591138	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	6	0.12192643771591138	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	6	0.12192643771591138	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	6	0.12192643771591138	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	6	0.12192643771591138	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	6	0.12192643771591138	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	6	0.12192643771591138	No Hit
GCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAAT	6	0.12192643771591138	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	6	0.12192643771591138	No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC	6	0.12192643771591138	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	6	0.12192643771591138	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	6	0.12192643771591138	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	6	0.12192643771591138	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	5	0.1016053647632595	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	5	0.1016053647632595	No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC	5	0.1016053647632595	No Hit
GTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACC	5	0.1016053647632595	No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT	5	0.1016053647632595	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	5	0.1016053647632595	No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA	5	0.1016053647632595	No Hit
GCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGG	5	0.1016053647632595	No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT	5	0.1016053647632595	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	5	0.1016053647632595	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	5	0.1016053647632595	No Hit
TCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCCATTG	5	0.1016053647632595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0406421459053038	0.0
28	0.0	0.0	0.0	0.2235318024791709	0.0
29	0.0	0.0	0.0	0.3861003861003861	0.0
30	0.0	0.0	0.0	0.5893111156269051	0.0
31	0.0	0.0	0.0	1.0770168664905506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCA	20	0.0016688555	37.0	10
>>END_MODULE