FastQCFastQC Report
Fri 10 Feb 2017
ERR1632483.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632483.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4784
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT511.0660535117056855Illumina PCR Primer Index 10 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA440.919732441471572No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA190.3971571906354515No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA180.3762541806020067No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT170.35535117056856186No Hit
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC160.33444816053511706No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC150.31354515050167225No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA140.2926421404682274No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT140.2926421404682274No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT140.2926421404682274No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT130.2717391304347826No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT130.2717391304347826No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC130.2717391304347826No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC130.2717391304347826No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC130.2717391304347826No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC120.2508361204013378No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC120.2508361204013378No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA120.2508361204013378No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA120.2508361204013378No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC120.2508361204013378No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC120.2508361204013378Illumina PCR Primer Index 10 (95% over 22bp)
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC120.2508361204013378No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA120.2508361204013378No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC110.229933110367893No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC110.229933110367893No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA110.229933110367893No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC110.229933110367893No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA100.20903010033444816No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG100.20903010033444816No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC100.20903010033444816No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT100.20903010033444816No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT90.18812709030100336No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG90.18812709030100336No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA90.18812709030100336No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC90.18812709030100336No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC90.18812709030100336No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG90.18812709030100336No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA90.18812709030100336No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG80.16722408026755853No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC80.16722408026755853No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT80.16722408026755853No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC80.16722408026755853No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG80.16722408026755853No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC80.16722408026755853No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC80.16722408026755853No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT80.16722408026755853No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA80.16722408026755853No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT70.1463210702341137No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC70.1463210702341137No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA70.1463210702341137No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG70.1463210702341137No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT70.1463210702341137No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC70.1463210702341137No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT70.1463210702341137No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC70.1463210702341137No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA70.1463210702341137No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT70.1463210702341137No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG70.1463210702341137No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA70.1463210702341137No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA70.1463210702341137No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA60.1254180602006689No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT60.1254180602006689No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC60.1254180602006689No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC60.1254180602006689No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT60.1254180602006689No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG60.1254180602006689No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG60.1254180602006689No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG60.1254180602006689No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC60.1254180602006689No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC60.1254180602006689No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA60.1254180602006689No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA60.1254180602006689No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC60.1254180602006689No Hit
GTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGAC60.1254180602006689No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG60.1254180602006689No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA60.1254180602006689No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT60.1254180602006689No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC60.1254180602006689No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC60.1254180602006689No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG60.1254180602006689No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT60.1254180602006689No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT60.1254180602006689No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA60.1254180602006689No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA60.1254180602006689No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT50.10451505016722408No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG50.10451505016722408No Hit
GCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGT50.10451505016722408No Hit
CGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACA50.10451505016722408No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA50.10451505016722408No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT50.10451505016722408No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT50.10451505016722408No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG50.10451505016722408No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT50.10451505016722408No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT50.10451505016722408No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA50.10451505016722408No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC50.10451505016722408No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA50.10451505016722408No Hit
GGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGC50.10451505016722408No Hit
GAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT50.10451505016722408No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA50.10451505016722408No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT50.10451505016722408No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC50.10451505016722408No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG50.10451505016722408No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA50.10451505016722408No Hit
CATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCA50.10451505016722408No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG50.10451505016722408No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG50.10451505016722408No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC50.10451505016722408No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA50.10451505016722408No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG50.10451505016722408No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC50.10451505016722408No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC50.10451505016722408No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGCTGT50.10451505016722408No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers