FastQCFastQC Report
Fri 10 Feb 2017
ERR1632482.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632482.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114378
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA2840.24829949815523963No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1830.15999580338876357No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1550.13551557117627516No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA1530.13376698316109742No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT1500.1311441011383308No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1470.1285212191155642No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1390.12152686705485322No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1370.11977827903967547No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1350.11802969102449772No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1300.11365822098655334No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1280.1119096329713756TruSeq Adapter, Index 3 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT1230.10753816293343126No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC1150.10054381087272028No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC1150.10054381087272028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGA200.001835013937.01
GGTATCA1400.031.7142851
GTACCTG250.00547592429.634
GAGAGCA250.00547592429.624
TGTAGAA250.00547592429.62
GAGCAAG250.00547592429.626
AGAGCAA250.00547592429.625
GATAGCT250.00547592429.637
TAGAACA358.8204397E-426.428574
CTCTATG358.8204397E-426.428571
TCTATGG358.8204397E-426.428572
TAATACG1150.025.73912827
GTAATAC1400.023.78571326
CGGTAAT1400.023.78571324
ATACACA551.8833076E-523.54545437
GCTAACT1500.023.4333322
CTATGGG400.001920628823.1250023
ACTTGGC400.001920628823.12500229
TATGGGC400.001920628823.1250024
GCGGTAA1450.022.96551723
Produced by FastQC (version 0.11.5)