##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632482.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	114378
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.07525922817325	34.0	33.0	34.0	31.0	34.0
2	33.21059994054801	34.0	33.0	34.0	31.0	34.0
3	33.282012275087865	34.0	34.0	34.0	31.0	34.0
4	36.558210495025264	37.0	37.0	37.0	35.0	37.0
5	36.5245501757331	37.0	37.0	37.0	35.0	37.0
6	36.57115878927766	37.0	37.0	37.0	35.0	37.0
7	36.57711273146934	37.0	37.0	37.0	35.0	37.0
8	36.55279861511829	37.0	37.0	37.0	35.0	37.0
9	38.35018097885957	39.0	39.0	39.0	37.0	39.0
10	38.382608543601044	39.0	39.0	39.0	37.0	39.0
11	38.45083844795328	39.0	39.0	39.0	37.0	39.0
12	38.41317386210635	39.0	39.0	39.0	37.0	39.0
13	38.44269877074262	39.0	39.0	39.0	37.0	39.0
14	40.01540506041371	41.0	40.0	41.0	38.0	41.0
15	39.999309307734	41.0	40.0	41.0	38.0	41.0
16	39.95736068124989	41.0	40.0	41.0	38.0	41.0
17	39.971445557712144	41.0	40.0	41.0	38.0	41.0
18	39.96850792984665	41.0	40.0	41.0	38.0	41.0
19	39.974689188480305	41.0	40.0	41.0	38.0	41.0
20	39.946633093776775	41.0	40.0	41.0	38.0	41.0
21	39.91114550000874	41.0	40.0	41.0	38.0	41.0
22	39.89246183706657	41.0	40.0	41.0	38.0	41.0
23	39.84898319606918	41.0	40.0	41.0	38.0	41.0
24	39.834268827921456	41.0	40.0	41.0	38.0	41.0
25	39.79207539911521	41.0	40.0	41.0	38.0	41.0
26	39.72352200598017	41.0	40.0	41.0	38.0	41.0
27	39.63910017660739	41.0	40.0	41.0	37.0	41.0
28	39.618291979226775	41.0	40.0	41.0	37.0	41.0
29	39.56571193761038	41.0	40.0	41.0	37.0	41.0
30	39.52028362097606	41.0	40.0	41.0	37.0	41.0
31	39.46731014705625	41.0	40.0	41.0	37.0	41.0
32	39.422642466208536	41.0	39.0	41.0	36.0	41.0
33	39.3545961635979	41.0	39.0	41.0	36.0	41.0
34	39.32466907971813	41.0	39.0	41.0	36.0	41.0
35	39.25116718250013	41.0	39.0	41.0	36.0	41.0
36	39.20955953067897	41.0	39.0	41.0	35.0	41.0
37	39.15296647956775	41.0	39.0	41.0	35.0	41.0
38	39.07014460822886	41.0	39.0	41.0	35.0	41.0
39	38.98613369703964	41.0	39.0	41.0	35.0	41.0
40	38.921794401021174	40.0	39.0	41.0	35.0	41.0
41	38.86068999283079	40.0	39.0	41.0	35.0	41.0
42	38.79870254769274	40.0	38.0	41.0	35.0	41.0
43	38.083853538267846	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	3.0
19	10.0
20	10.0
21	13.0
22	27.0
23	42.0
24	57.0
25	94.0
26	92.0
27	179.0
28	241.0
29	347.0
30	414.0
31	622.0
32	796.0
33	1076.0
34	1625.0
35	2632.0
36	4113.0
37	8045.0
38	21420.0
39	72518.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.18312962282956	16.201542254629388	12.686006050114532	27.929322072426515
2	21.683365682211615	19.02026613509591	31.959817447411215	27.33655073528126
3	21.255835912500658	19.799262095857596	28.703072269142666	30.24182972249908
4	16.163073318295478	15.358722831313715	32.96350696812324	35.51469688226757
5	17.542709262270716	32.76504222840057	32.78515099057511	16.907097518753606
6	35.26377450208956	33.21880081833919	14.79655178443407	16.720872895137177
7	30.692091136407353	27.116228645368867	19.45391596285999	22.737764255363793
8	27.43010019409327	30.776897655143472	19.807130741925892	21.98587140883736
9	27.619821993740057	13.056706709332214	17.248946475720857	42.07452482120688
10	19.5081221913305	23.261466365909527	29.533651576352092	27.696759866407877
11	39.49448320481211	19.328891919774783	18.369791393449788	22.806833481963317
12	23.015789749777056	23.255346307856406	26.36171291681967	27.36715102554687
13	34.80564444211299	17.55844655440732	20.77497420832678	26.860934795152914
14	24.535312734966514	19.084963891657488	22.613614506286172	33.76610886708982
15	29.648184091346234	23.841997586948537	19.897183024707548	26.612635296997674
16	26.568046302610643	23.674133137491477	21.868716011820453	27.889104548077427
17	27.3723967895924	23.373375998880906	21.52424417283044	27.729983038696254
18	26.511217192117364	21.55397016908846	23.217751665530084	28.71706097326409
19	28.473132945146794	22.445750056829112	22.63896903250625	26.442147965517844
20	29.531902988336917	20.79420867649373	22.736015667348617	26.937872667820734
21	27.818286733462728	22.193079088635926	21.738446204689712	28.25018797321163
22	28.318382905803563	22.631100386437954	21.00316494430747	28.04735176345101
23	28.254559443249576	22.55328821976254	21.81276119533477	27.379391141653116
24	28.474007239154382	21.691234328279915	21.696480092325444	28.13827834024026
25	28.851702250432776	21.769920788962914	22.458864466942945	26.91951249366137
26	28.373463428281664	22.669569322771864	21.976254174753887	26.980713074192593
27	27.761457622969456	21.623039395687982	23.28332371609925	27.33217926524332
28	26.464879609715155	22.807707775970904	22.124884156043993	28.602528458269948
29	27.69151410236234	23.29031806815996	21.881830421934286	27.136337407543408
30	27.162566227771073	22.179090384514506	23.503645806011644	27.154697581702774
31	28.083197817762155	21.855601601706624	22.466733113011244	27.59446746751998
32	25.682386472923113	22.074175103603842	22.990435223556975	29.253003199916066
33	26.31362674640228	21.63877668782458	24.016856388466312	28.030740177306825
34	26.722796341953874	21.450803476192977	23.83412894088024	27.992271240972915
35	26.25155187186347	22.45361870289741	24.15849201769571	27.136337407543408
36	26.29963804228086	21.216492682159156	24.864047281819932	27.619821993740057
37	26.617881061043207	20.52405182814877	24.827326933501197	28.030740177306825
38	25.75670146356817	20.000349717603036	26.30838098235675	27.934567836472045
39	24.89464757208554	19.382223854237704	27.475563482487892	28.247565091188864
40	24.47673503645806	20.132368112748956	28.58591687212576	26.804979978667227
41	22.74825578345486	19.449544492822046	29.508297050132022	28.293902673591077
42	21.62566227771075	20.360558848729653	30.141285911626365	27.872492961933236
43	21.053873996747626	20.727762331916978	29.889489237440763	28.32887443389463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	2.0
4	3.0
5	3.0
6	3.0
7	3.0
8	4.5
9	6.0
10	6.0
11	6.0
12	6.0
13	17.5
14	29.0
15	58.0
16	87.0
17	83.5
18	80.0
19	80.0
20	99.0
21	118.0
22	110.0
23	102.0
24	137.5
25	173.0
26	173.0
27	203.5
28	234.0
29	357.5
30	481.0
31	572.0
32	663.0
33	663.0
34	967.0
35	1271.0
36	1434.0
37	1597.0
38	2189.5
39	2782.0
40	2782.0
41	3318.0
42	3854.0
43	4409.0
44	4964.0
45	6255.5
46	7547.0
47	7547.0
48	8601.5
49	9656.0
50	10954.5
51	12253.0
52	12881.5
53	13510.0
54	13510.0
55	12700.0
56	11890.0
57	11231.0
58	10572.0
59	9752.0
60	8932.0
61	8932.0
62	8302.5
63	7673.0
64	6407.0
65	5141.0
66	4399.5
67	3658.0
68	3658.0
69	3154.0
70	2650.0
71	2227.5
72	1805.0
73	1455.0
74	1105.0
75	1105.0
76	895.5
77	686.0
78	526.0
79	366.0
80	295.5
81	225.0
82	225.0
83	179.5
84	134.0
85	97.5
86	61.0
87	44.5
88	28.0
89	28.0
90	24.5
91	21.0
92	15.5
93	10.0
94	5.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	114378.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.65152389445523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.29994810586403	52.342233646330584
2	6.129274058698034	7.434996240535767
3	2.2473043879374965	4.089073073493154
4	1.1921236233638932	2.8921645771039883
5	0.8461627169463184	2.5660529122733395
6	0.5621864729285591	2.04584797775796
7	0.42524361413826905	1.8054171256710205
8	0.3805569970593323	1.8465089440276976
9	0.24505564204578217	1.3376698316109743
>10	1.5539410713256068	17.71931665180367
>50	0.0908147379346134	3.628320131493819
>100	0.027388571758058006	2.2923988878980226
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	284	0.24829949815523963	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	183	0.15999580338876357	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	155	0.13551557117627516	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	153	0.13376698316109742	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	150	0.1311441011383308	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	147	0.1285212191155642	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	139	0.12152686705485322	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	137	0.11977827903967547	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	135	0.11802969102449772	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	130	0.11365822098655334	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	128	0.1119096329713756	TruSeq Adapter, Index 3 (95% over 21bp)
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	123	0.10753816293343126	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	115	0.10054381087272028	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	115	0.10054381087272028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	8.74294007588872E-4	0.0	0.0	0.0	0.0
16	8.74294007588872E-4	0.0	0.0	8.74294007588872E-4	0.0
17	8.74294007588872E-4	0.0	0.0	8.74294007588872E-4	0.0
18	8.74294007588872E-4	0.0	0.0	0.001748588015177744	0.0
19	8.74294007588872E-4	0.0	0.0	0.001748588015177744	0.0
20	8.74294007588872E-4	0.0	0.0	0.001748588015177744	0.0
21	8.74294007588872E-4	0.0	0.0	0.002622882022766616	0.0
22	8.74294007588872E-4	0.0	0.0	0.005245764045533232	0.0
23	8.74294007588872E-4	0.0	0.0	0.006120058053122104	0.0
24	8.74294007588872E-4	0.0	0.0	0.00874294007588872	0.0
25	8.74294007588872E-4	0.0	0.0	0.012240116106244208	0.0
26	8.74294007588872E-4	0.0	0.0	0.01661158614418857	0.0
27	8.74294007588872E-4	0.0	0.0	0.04808617041738796	0.0
28	8.74294007588872E-4	0.0	0.0	0.16436727342670793	0.0
29	8.74294007588872E-4	0.0	0.0	0.354963367081082	0.0
30	8.74294007588872E-4	0.0	0.0	0.5866512790921331	0.0
31	8.74294007588872E-4	0.0	0.0	1.2414974907761982	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTAGA	20	0.0018350139	37.0	1
GGTATCA	140	0.0	31.714285	1
GTACCTG	25	0.005475924	29.6	34
GAGAGCA	25	0.005475924	29.6	24
TGTAGAA	25	0.005475924	29.6	2
GAGCAAG	25	0.005475924	29.6	26
AGAGCAA	25	0.005475924	29.6	25
GATAGCT	25	0.005475924	29.6	37
TAGAACA	35	8.8204397E-4	26.42857	4
CTCTATG	35	8.8204397E-4	26.42857	1
TCTATGG	35	8.8204397E-4	26.42857	2
TAATACG	115	0.0	25.739128	27
GTAATAC	140	0.0	23.785713	26
CGGTAAT	140	0.0	23.785713	24
ATACACA	55	1.8833076E-5	23.545454	37
GCTAACT	150	0.0	23.433332	2
CTATGGG	40	0.0019206288	23.125002	3
ACTTGGC	40	0.0019206288	23.125002	29
TATGGGC	40	0.0019206288	23.125002	4
GCGGTAA	145	0.0	22.965517	23
>>END_MODULE