FastQCFastQC Report
Fri 10 Feb 2017
ERR1632481.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632481.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4087
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT160.3914851969659897RNA PCR Primer, Index 41 (95% over 22bp)
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC130.31808172253486666RNA PCR Primer, Index 41 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA100.24467824810374358No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA90.2202104232933692No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA90.2202104232933692No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT80.19574259848299486No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA80.19574259848299486No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT70.1712747736726205No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT70.1712747736726205No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC70.1712747736726205No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC70.1712747736726205No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA70.1712747736726205No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA60.14680694886224616No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC60.14680694886224616No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT50.12233912405187179No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC50.12233912405187179No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA50.12233912405187179No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG50.12233912405187179No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT50.12233912405187179No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA50.12233912405187179No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50.12233912405187179No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC50.12233912405187179No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC50.12233912405187179No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT50.12233912405187179No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA50.12233912405187179No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC50.12233912405187179No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT50.12233912405187179No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)