##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632481.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4087
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00391485196966	34.0	33.0	34.0	31.0	34.0
2	33.158551504771225	34.0	33.0	34.0	31.0	34.0
3	33.227061414240275	34.0	33.0	34.0	31.0	34.0
4	36.5162711034989	37.0	37.0	37.0	35.0	37.0
5	36.46072914117935	37.0	37.0	37.0	35.0	37.0
6	36.534377293858576	37.0	37.0	37.0	35.0	37.0
7	36.49889894788353	37.0	37.0	37.0	35.0	37.0
8	36.486175678982136	37.0	37.0	37.0	35.0	37.0
9	38.29092243699535	39.0	39.0	39.0	37.0	39.0
10	38.312209444580375	39.0	39.0	39.0	37.0	39.0
11	38.40249571813066	39.0	39.0	39.0	37.0	39.0
12	38.37166625886959	39.0	39.0	39.0	37.0	39.0
13	38.376804502079764	39.0	39.0	39.0	37.0	39.0
14	39.892586249082456	41.0	40.0	41.0	38.0	41.0
15	39.91729875214094	41.0	40.0	41.0	38.0	41.0
16	39.895277709811594	41.0	40.0	41.0	38.0	41.0
17	39.93100073403475	41.0	40.0	41.0	38.0	41.0
18	39.85931000734035	41.0	40.0	41.0	38.0	41.0
19	39.84120381698067	41.0	40.0	41.0	38.0	41.0
20	39.868607780768286	41.0	40.0	41.0	38.0	41.0
21	39.82260827012479	41.0	40.0	41.0	38.0	41.0
22	39.83973574749205	41.0	40.0	41.0	38.0	41.0
23	39.75336432591143	41.0	40.0	41.0	38.0	41.0
24	39.72057744066552	41.0	40.0	41.0	38.0	41.0
25	39.71715194519207	41.0	40.0	41.0	37.0	41.0
26	39.5294837288965	41.0	40.0	41.0	37.0	41.0
27	39.48837778321507	41.0	39.0	41.0	37.0	41.0
28	39.3924639099584	41.0	39.0	41.0	37.0	41.0
29	39.29679471494984	41.0	39.0	41.0	36.0	41.0
30	39.29336921947639	41.0	39.0	41.0	36.0	41.0
31	39.26669929043308	41.0	39.0	41.0	36.0	41.0
32	39.2219231710301	41.0	39.0	41.0	36.0	41.0
33	39.13334964521654	41.0	39.0	41.0	35.0	41.0
34	39.13016882799119	41.0	39.0	41.0	35.0	41.0
35	39.02299975532175	41.0	39.0	41.0	35.0	41.0
36	38.94323464643993	41.0	39.0	41.0	35.0	41.0
37	38.87007585025691	41.0	39.0	41.0	35.0	41.0
38	38.76070467335454	40.0	38.0	41.0	35.0	41.0
39	38.63518473207732	40.0	38.0	41.0	35.0	41.0
40	38.54661120626376	40.0	38.0	41.0	35.0	41.0
41	38.51969659897235	40.0	38.0	41.0	35.0	41.0
42	38.44776119402985	40.0	38.0	41.0	35.0	41.0
43	37.72963053584537	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	0.0
22	3.0
23	3.0
24	4.0
25	4.0
26	7.0
27	9.0
28	13.0
29	10.0
30	21.0
31	20.0
32	33.0
33	55.0
34	58.0
35	116.0
36	168.0
37	315.0
38	812.0
39	2435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.769757768534376	15.586004404208467	13.01688279911916	28.627355028137995
2	20.871054563249327	19.182774651333496	32.10178615121116	27.844384634206023
3	21.26253976021532	19.965745045265475	27.79544898458527	30.97626620993394
4	16.05089307560558	14.974308783949105	30.48690971372645	38.48788842671886
5	16.540249571813064	31.979447027159285	34.230486909713726	17.249816491313922
6	36.18791289454367	32.5422069977979	14.925373134328357	16.34450697333007
7	30.60924883777832	26.596525568876928	19.476388549057987	23.31783704428676
8	28.529483728896498	31.220944458037682	18.25299730853927	21.99657450452655
9	27.819916809395647	13.041350623929532	16.73599217029606	42.40274039637876
10	21.017861512111573	22.730609248837776	28.65182285294837	27.599706386102273
11	39.588940543185714	18.840225103988256	17.983851235625153	23.58698311720088
12	23.929532664546123	22.779544898458525	24.98164913139222	28.309273305603135
13	35.25813555174945	17.00513824321018	19.427452899437238	28.309273305603135
14	25.397602153168584	19.158306826523123	21.580621482750185	33.86346953755811
15	30.853927085882066	24.002936138977248	17.959383410814777	27.183753364325913
16	28.01565940787864	23.709322241252753	20.528505015904088	27.746513334964522
17	29.018840225103986	23.41570834352826	19.280645950574993	28.28480548079276
18	27.991191583068264	20.234891118179593	22.779544898458525	28.994372400293617
19	29.4837288965011	21.55615365793981	22.33912405187179	26.620993393687304
20	29.997553217518963	20.577440665524836	21.21360411059457	28.211402006361634
21	28.74969415218987	21.384878884267188	21.28700758502569	28.57841937851725
22	28.65182285294837	23.07315879618302	20.455101541472963	27.819916809395647
23	28.602887203327626	22.950819672131146	21.874235380474676	26.57205774406655
24	29.434793246880353	21.04232933692195	20.455101541472963	29.067775874724738
25	28.994372400293617	22.485931000734034	20.84658673843895	27.673109860533394
26	28.700758502569123	22.534866650354786	20.748715439197454	28.01565940787864
27	27.550770736481528	22.265720577440664	23.391240518717886	26.79226816735992
28	28.260337655982383	20.577440665524836	23.122094445803768	28.040127232689017
29	27.86885245901639	23.268901394666013	21.238071935404943	27.62417421091265
30	28.162466356740882	22.02104232933692	21.947638854905797	27.86885245901639
31	30.119892341570836	20.944458037680448	21.482750183508685	27.45289943724003
32	26.914607291411798	21.189136285784194	21.99657450452655	29.899681918277466
33	25.642280401272327	23.07315879618302	24.247614387080986	27.036946415463664
34	27.183753364325913	21.50721800831906	21.384878884267188	29.924149743087842
35	27.306092488377782	20.8955223880597	24.443356985563984	27.355028137998534
36	27.673109860533394	21.26253976021532	23.29336921947639	27.770981159774895
37	28.01565940787864	20.479569366283336	24.296550036701735	27.20822118913628
38	28.18693418155126	20.84658673843895	24.198678737460238	26.767800342549545
39	25.61781257646195	20.871054563249327	25.789087350134572	27.722045510154146
40	24.198678737460238	20.21042329336922	27.33056031318816	28.260337655982383
41	25.030584781012966	20.406165891852215	27.012478590653288	27.550770736481528
42	23.07315879618302	21.238071935404943	28.064595057499385	27.62417421091265
43	22.045510154147298	20.8955223880597	28.211402006361634	28.847565451431368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	2.0
12	2.0
13	1.5
14	1.0
15	3.5
16	6.0
17	5.0
18	4.0
19	4.0
20	3.0
21	2.0
22	1.5
23	1.0
24	3.5
25	6.0
26	6.0
27	6.5
28	7.0
29	9.5
30	12.0
31	14.0
32	16.0
33	16.0
34	26.0
35	36.0
36	46.0
37	56.0
38	67.5
39	79.0
40	79.0
41	96.0
42	113.0
43	137.5
44	162.0
45	206.0
46	250.0
47	250.0
48	292.5
49	335.0
50	354.5
51	374.0
52	401.0
53	428.0
54	428.0
55	438.0
56	448.0
57	438.5
58	429.0
59	380.5
60	332.0
61	332.0
62	304.5
63	277.0
64	249.5
65	222.0
66	188.0
67	154.0
68	154.0
69	138.5
70	123.0
71	101.0
72	79.0
73	64.0
74	49.0
75	49.0
76	44.0
77	39.0
78	30.0
79	21.0
80	15.5
81	10.0
82	10.0
83	7.0
84	4.0
85	4.0
86	4.0
87	3.5
88	3.0
89	3.0
90	2.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4087.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.81502324443356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.4212872666481	81.15977489601175
2	4.848147116188353	8.514803034010276
3	1.3095569796600723	3.449963298262784
4	0.6687099470604625	2.3489111817959385
5	0.3622178879910839	1.5904086126743333
6	0.05572582892170522	0.2936138977244923
7	0.13931457230426303	0.8563738683631025
8	0.05572582892170522	0.3914851969659897
9	0.05572582892170522	0.4404208465867384
>10	0.08358874338255781	0.9542451676045999
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	16	0.3914851969659897	RNA PCR Primer, Index 41 (95% over 22bp)
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	13	0.31808172253486666	RNA PCR Primer, Index 41 (95% over 23bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	10	0.24467824810374358	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	9	0.2202104232933692	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	9	0.2202104232933692	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	8	0.19574259848299486	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	8	0.19574259848299486	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	7	0.1712747736726205	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	7	0.1712747736726205	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	7	0.1712747736726205	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	7	0.1712747736726205	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	7	0.1712747736726205	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	6	0.14680694886224616	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	6	0.14680694886224616	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	5	0.12233912405187179	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	5	0.12233912405187179	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	5	0.12233912405187179	No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG	5	0.12233912405187179	No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT	5	0.12233912405187179	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	5	0.12233912405187179	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.12233912405187179	No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC	5	0.12233912405187179	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	5	0.12233912405187179	No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT	5	0.12233912405187179	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	5	0.12233912405187179	No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC	5	0.12233912405187179	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	5	0.12233912405187179	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.14680694886224616	0.0
29	0.0	0.0	0.0	0.2936138977244923	0.0
30	0.0	0.0	0.0	0.48935649620748717	0.0
31	0.0	0.0	0.0	0.7585025691216051	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE