FastQCFastQC Report
Fri 10 Feb 2017
ERR1632480.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632480.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6204
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA200.3223726627981947No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA190.30625402965828497No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCGGGCTGCGTCT170.2740167633784655No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT160.2578981302385558RNA PCR Primer, Index 33 (95% over 22bp)
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA150.24177949709864605No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA140.2256608639587363No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC130.20954223081882656No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC130.20954223081882656No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC120.19342359767891684RNA PCR Primer, Index 33 (95% over 23bp)
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA110.1773049645390071No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT110.1773049645390071No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGGTCTT100.16118633139909735No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC90.14506769825918764No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA90.14506769825918764No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT80.1289490651192779No Hit
GTGTGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC80.1289490651192779No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC80.1289490651192779No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA80.1289490651192779No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA80.1289490651192779No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA80.1289490651192779No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA80.1289490651192779No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT70.11283043197936815No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC70.11283043197936815No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC70.11283043197936815No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT70.11283043197936815No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC70.11283043197936815No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT70.11283043197936815No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG70.11283043197936815No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT70.11283043197936815No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG70.11283043197936815No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG70.11283043197936815No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA70.11283043197936815No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)