FastQCFastQC Report
Fri 10 Feb 2017
ERR1632479.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632479.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10266
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT660.6428988895382818TruSeq Adapter, Index 5 (95% over 21bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA620.6039353204753555No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA500.487044613286577No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA410.39937658289499317No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT350.34093122930060393No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA330.3214494447691409No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA300.29222676797194624No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA280.27274498344048315No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC270.2630040911747516No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA270.2630040911747516No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC260.2532631989090201No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT230.22404052211182543No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA230.22404052211182543No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC230.22404052211182543No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC230.22404052211182543No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT220.2142996298460939No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT220.2142996298460939No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG220.2142996298460939No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT210.20455873758036236No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC210.20455873758036236No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC200.19481784531463084No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC200.19481784531463084No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC190.18507695304889926No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA190.18507695304889926No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA190.18507695304889926No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA190.18507695304889926No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA190.18507695304889926No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC190.18507695304889926No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA190.18507695304889926No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC180.17533606078316774Illumina Paired End PCR Primer 2 (95% over 23bp)
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC180.17533606078316774No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG180.17533606078316774No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC180.17533606078316774No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG180.17533606078316774No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA170.1655951685174362No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG170.1655951685174362No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA170.1655951685174362No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC170.1655951685174362No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC170.1655951685174362No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA160.15585427625170464No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC160.15585427625170464No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG160.15585427625170464No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC160.15585427625170464No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA160.15585427625170464No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG160.15585427625170464No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC160.15585427625170464No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT160.15585427625170464No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA160.15585427625170464No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC150.14611338398597312No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC150.14611338398597312No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA150.14611338398597312No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA150.14611338398597312No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG150.14611338398597312No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT150.14611338398597312No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG150.14611338398597312No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT150.14611338398597312No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC140.13637249172024157No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG140.13637249172024157No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC140.13637249172024157No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC140.13637249172024157No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT140.13637249172024157No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA140.13637249172024157No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT140.13637249172024157No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC130.12663159945451005No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC130.12663159945451005No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC130.12663159945451005No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT130.12663159945451005No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC130.12663159945451005No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA130.12663159945451005No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG130.12663159945451005No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT130.12663159945451005No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT120.11689070718877849No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA120.11689070718877849No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA120.11689070718877849No Hit
GAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTACC120.11689070718877849No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT120.11689070718877849No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG120.11689070718877849No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA120.11689070718877849No Hit
ACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGCATCAGGC110.10714981492304695No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG110.10714981492304695No Hit
TGTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGG110.10714981492304695No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT110.10714981492304695No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC110.10714981492304695No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG110.10714981492304695No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC110.10714981492304695No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT110.10714981492304695No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA110.10714981492304695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110.10714981492304695No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG110.10714981492304695No Hit
GAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAAC110.10714981492304695No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG110.10714981492304695No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA110.10714981492304695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCA303.358794E-430.83333228
TAACGTC303.358794E-430.83333227
GTAACGT303.358794E-430.83333226
CGTCAAT250.005249114729.630
CAATGAG250.005249114729.633
GTCAATG250.005249114729.631
TTCTGCG250.005249114729.618
TCAATGA250.005249114729.632
GAGCAAA250.005249114729.637
CGGGTAA250.005249114729.623
TCTGCGG250.005249114729.619
CTGCGGG250.005249114729.620
TTCTTCT250.005249114729.615
TCTTCTG250.005249114729.616
GCTTCTT250.005249114729.613
TGAGCAA250.005249114729.636
TGCGGGT250.005249114729.621
CTTCTGC250.005249114729.617
AATGAGC250.005249114729.634
ATGAGCA250.005249114729.635
Produced by FastQC (version 0.11.5)