FastQCFastQC Report
Fri 10 Feb 2017
ERR1632478.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632478.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT110.7638888888888888TruSeq Adapter, Index 10 (95% over 21bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA80.5555555555555556No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA60.4166666666666667No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC60.4166666666666667No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT50.3472222222222222No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC50.3472222222222222RNA PCR Primer, Index 10 (95% over 22bp)
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC50.3472222222222222No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA50.3472222222222222No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA50.3472222222222222No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA50.3472222222222222No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC40.2777777777777778No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA40.2777777777777778No Hit
TCCTTCATCGCCTCTGACTGCCAGGGCATCCACCGTGTACGCT40.2777777777777778No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC40.2777777777777778No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT40.2777777777777778No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA30.20833333333333334No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG30.20833333333333334No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC30.20833333333333334No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT30.20833333333333334No Hit
GCTAAATACTCCTGACTGACCGATAGTGAACCAGTACCGTGAG30.20833333333333334No Hit
CTTCAGCACCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATT30.20833333333333334No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT30.20833333333333334No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA30.20833333333333334No Hit
CTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTA30.20833333333333334No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30.20833333333333334No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC30.20833333333333334No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC30.20833333333333334No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC30.20833333333333334No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA30.20833333333333334No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC30.20833333333333334No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT30.20833333333333334No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG30.20833333333333334No Hit
ACGTTAGAACATCAAACATTAAAGGGTGGTATTTCAAGGTCGG30.20833333333333334No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT30.20833333333333334No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA30.20833333333333334No Hit
GCGCAAGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCT30.20833333333333334No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT30.20833333333333334No Hit
GGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCA30.20833333333333334No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC30.20833333333333334No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC30.20833333333333334No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC30.20833333333333334No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA30.20833333333333334No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC30.20833333333333334No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT30.20833333333333334No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA20.1388888888888889No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC20.1388888888888889No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT20.1388888888888889No Hit
CATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACT20.1388888888888889No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA20.1388888888888889No Hit
CTCACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTA20.1388888888888889No Hit
GCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTAACAATAAC20.1388888888888889No Hit
GTCCTCGTTCATCTGATCAGGATCACTGAGTGGGTCTGCCTGG20.1388888888888889No Hit
CCGTAAACGATGTCGACTTGGAGGTTGTGCCCTTGAGGCGTGG20.1388888888888889No Hit
TATCAACGCAGAGTACGGGCGGAAAGACCCCTGTCTCTTATAC20.1388888888888889No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA20.1388888888888889No Hit
GAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCAC20.1388888888888889No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCTGTCTC20.1388888888888889No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT20.1388888888888889No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA20.1388888888888889No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA20.1388888888888889No Hit
ATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCCTTTG20.1388888888888889No Hit
AGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATA20.1388888888888889No Hit
GCGTAATGATGGCCAGGCTGTCTCCACCCGAGACTCAGTGAAA20.1388888888888889No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT20.1388888888888889No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC20.1388888888888889No Hit
CTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTAC20.1388888888888889No Hit
ACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC20.1388888888888889No Hit
GTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAAA20.1388888888888889No Hit
TGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGA20.1388888888888889No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA20.1388888888888889No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG20.1388888888888889No Hit
TTCTTCATACACGCGGCATGGCTGCATCAGGCTTGCGCCTGTC20.1388888888888889No Hit
GTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCA20.1388888888888889No Hit
GGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCC20.1388888888888889No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT20.1388888888888889No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGGCGGTTT20.1388888888888889No Hit
ATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCC20.1388888888888889No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG20.1388888888888889No Hit
GCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGTAC20.1388888888888889No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT20.1388888888888889No Hit
GCCCAGAGCCTGAATCAGTATGTGTGTTAGTGGAAGCGTCTGG20.1388888888888889No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC20.1388888888888889No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG20.1388888888888889No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC20.1388888888888889No Hit
ACCATAGAGCAAAGAGCTAAGGATCATCAGCAAAGGCCCGCTG20.1388888888888889No Hit
GCCAGGCTGTCTCCACCCGAGACTCAGTGAAATTGAACTCGCT20.1388888888888889No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGG20.1388888888888889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20.1388888888888889No Hit
AACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAACGC20.1388888888888889No Hit
GCTTAGAAGTGTGGACGCCAGTCTGCATGGAGCCGACCTTGAA20.1388888888888889No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCCC20.1388888888888889No Hit
GTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT20.1388888888888889No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGC20.1388888888888889No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCCTGTCTC20.1388888888888889No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG20.1388888888888889No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA20.1388888888888889No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCCTGTCT20.1388888888888889No Hit
GCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGT20.1388888888888889No Hit
GGTAGAGAATACCAAGGCGCTTGAGAGAACTCGGGTGAAGGAA20.1388888888888889No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC20.1388888888888889No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG20.1388888888888889No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT20.1388888888888889No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT20.1388888888888889No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG20.1388888888888889No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG20.1388888888888889No Hit
CATTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT20.1388888888888889No Hit
ATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAG20.1388888888888889No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG20.1388888888888889No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG20.1388888888888889No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA20.1388888888888889No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA20.1388888888888889No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC20.1388888888888889No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)