FastQCFastQC Report
Fri 10 Feb 2017
ERR1632477.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632477.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20419
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA790.38689455898917674No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA590.28894656937166363No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT580.284049169890788TruSeq Adapter, Index 7 (95% over 21bp)
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA480.23507517508203143No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA420.2056907781967775No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT420.2056907781967775No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA400.1958959792350262No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA400.1958959792350262No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA390.19099857975415055No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC380.18610118027327488No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT380.18610118027327488No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG380.18610118027327488No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT360.17630638131152357No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA350.17140898183064793No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT330.16161418286889662No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT320.15671678338802097No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT310.1518193839071453No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC310.1518193839071453No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC310.1518193839071453No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA300.14692198442626966No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA280.13712718546451835No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT260.12733238650276704No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA260.12733238650276704No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG260.12733238650276704No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA250.12243498702189136No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC240.11753758754101572No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC240.11753758754101572No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC240.11753758754101572No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC240.11753758754101572No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA240.11753758754101572No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA230.11264018806014006No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG230.11264018806014006No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA220.1077427885792644No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC220.1077427885792644No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC220.1077427885792644No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC210.10284538909838875No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA210.10284538909838875No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG210.10284538909838875No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG210.10284538909838875No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA210.10284538909838875No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC210.10284538909838875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACT200.001799886237.04
TCTTATA500.0068064318.537
TGTCTCT1200.00676414910.79166637
Produced by FastQC (version 0.11.5)