FastQCFastQC Report
Fri 10 Feb 2017
ERR1632476.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632476.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2192
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA90.41058394160583944No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT80.36496350364963503RNA PCR Primer, Index 39 (95% over 23bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT70.3193430656934307No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA70.3193430656934307No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA70.3193430656934307No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG60.2737226277372263No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT50.22810218978102187No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA40.18248175182481752No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA40.18248175182481752No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC40.18248175182481752No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG40.18248175182481752No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA40.18248175182481752No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG40.18248175182481752No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC40.18248175182481752No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC40.18248175182481752No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC40.18248175182481752No Hit
CCTTGGTATTCTCTACCTGACCACCTGTGTCGGTTTGGGGTAC30.13686131386861314No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGG30.13686131386861314No Hit
CCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGT30.13686131386861314No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC30.13686131386861314RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC30.13686131386861314RNA PCR Primer, Index 39 (95% over 21bp)
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT30.13686131386861314No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA30.13686131386861314No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG30.13686131386861314No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA30.13686131386861314No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTT30.13686131386861314No Hit
GTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT30.13686131386861314No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG30.13686131386861314No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT30.13686131386861314No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC30.13686131386861314No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA30.13686131386861314No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC30.13686131386861314No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC30.13686131386861314No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC30.13686131386861314No Hit
CAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT30.13686131386861314No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA30.13686131386861314No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG30.13686131386861314No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA30.13686131386861314No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA30.13686131386861314No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT30.13686131386861314No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT30.13686131386861314No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA30.13686131386861314No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC30.13686131386861314No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT30.13686131386861314No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC30.13686131386861314No Hit
GGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCA30.13686131386861314No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA30.13686131386861314No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers