##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632476.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2192
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.00045620437956	34.0	33.0	34.0	31.0	34.0
2	33.19388686131387	34.0	33.0	34.0	31.0	34.0
3	33.254105839416056	34.0	34.0	34.0	31.0	34.0
4	36.5	37.0	37.0	37.0	35.0	37.0
5	36.50684306569343	37.0	37.0	37.0	35.0	37.0
6	36.5492700729927	37.0	37.0	37.0	35.0	37.0
7	36.5168795620438	37.0	37.0	37.0	35.0	37.0
8	36.50821167883212	37.0	37.0	37.0	35.0	37.0
9	38.30155109489051	39.0	39.0	39.0	37.0	39.0
10	38.38275547445255	39.0	39.0	39.0	37.0	39.0
11	38.34260948905109	39.0	39.0	39.0	37.0	39.0
12	38.387317518248175	39.0	39.0	39.0	37.0	39.0
13	38.3581204379562	39.0	39.0	39.0	37.0	39.0
14	39.88229927007299	41.0	40.0	41.0	38.0	41.0
15	39.96350364963504	41.0	40.0	41.0	38.0	41.0
16	39.88503649635037	41.0	40.0	41.0	38.0	41.0
17	39.91970802919708	41.0	40.0	41.0	38.0	41.0
18	39.95255474452555	41.0	40.0	41.0	38.0	41.0
19	39.940237226277375	41.0	40.0	41.0	38.0	41.0
20	39.91560218978102	41.0	40.0	41.0	38.0	41.0
21	39.82664233576642	41.0	40.0	41.0	38.0	41.0
22	39.85857664233577	41.0	40.0	41.0	38.0	41.0
23	39.65875912408759	41.0	40.0	41.0	38.0	41.0
24	39.7271897810219	41.0	40.0	41.0	38.0	41.0
25	39.63184306569343	41.0	40.0	41.0	37.0	41.0
26	39.63549270072993	41.0	40.0	41.0	37.0	41.0
27	39.46076642335766	41.0	40.0	41.0	37.0	41.0
28	39.3882299270073	41.0	39.0	41.0	37.0	41.0
29	39.394616788321166	41.0	40.0	41.0	36.0	41.0
30	39.3389598540146	41.0	39.0	41.0	36.0	41.0
31	39.26459854014598	41.0	39.0	41.0	35.0	41.0
32	39.24315693430657	41.0	39.0	41.0	36.0	41.0
33	39.128649635036496	41.0	39.0	41.0	35.0	41.0
34	39.128193430656935	41.0	39.0	41.0	35.0	41.0
35	39.026916058394164	41.0	39.0	41.0	35.0	41.0
36	38.97536496350365	41.0	39.0	41.0	35.0	41.0
37	38.887773722627735	40.0	39.0	41.0	35.0	41.0
38	38.78832116788321	40.0	38.0	41.0	35.0	41.0
39	38.71943430656934	40.0	38.0	41.0	35.0	41.0
40	38.67107664233577	40.0	38.0	41.0	35.0	41.0
41	38.683850364963504	40.0	38.0	41.0	35.0	41.0
42	38.48357664233577	40.0	38.0	41.0	35.0	41.0
43	37.730839416058394	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	1.0
23	3.0
24	0.0
25	0.0
26	2.0
27	4.0
28	9.0
29	6.0
30	7.0
31	19.0
32	17.0
33	22.0
34	29.0
35	74.0
36	92.0
37	158.0
38	421.0
39	1327.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.01642335766424	17.335766423357665	13.822992700729927	26.824817518248175
2	21.897810218978105	18.43065693430657	31.25	28.42153284671533
3	21.076642335766422	19.61678832116788	27.874087591240876	31.43248175182482
4	14.826642335766424	15.602189781021897	34.62591240875913	34.94525547445255
5	16.6514598540146	33.75912408759124	32.57299270072993	17.016423357664234
6	34.99087591240876	32.57299270072993	14.507299270072993	17.92883211678832
7	30.702554744525546	27.0985401459854	18.613138686131386	23.585766423357665
8	26.733576642335766	31.52372262773723	19.434306569343065	22.30839416058394
9	27.326642335766422	13.777372262773724	16.33211678832117	42.56386861313868
10	20.346715328467152	22.673357664233578	29.516423357664234	27.463503649635037
11	39.50729927007299	18.97810218978102	18.97810218978102	22.536496350364963
12	24.13321167883212	23.996350364963504	23.9507299270073	27.919708029197082
13	34.62591240875913	16.514598540145986	21.852189781021895	27.00729927007299
14	26.003649635036496	18.704379562043798	23.220802919708028	32.07116788321168
15	30.793795620437958	25.09124087591241	18.704379562043798	25.41058394160584
16	26.551094890510953	23.494525547445257	21.44160583941606	28.51277372262774
17	30.885036496350367	23.12956204379562	19.844890510948904	26.14051094890511
18	26.551094890510953	22.445255474452555	20.93978102189781	30.063868613138684
19	30.01824817518248	21.806569343065693	21.852189781021895	26.322992700729923
20	31.34124087591241	19.753649635036496	22.30839416058394	26.596715328467152
21	28.42153284671533	19.936131386861312	21.395985401459853	30.246350364963504
22	28.056569343065696	22.673357664233578	19.936131386861312	29.333941605839414
23	28.558394160583944	22.490875912408757	20.30109489051095	28.649635036496353
24	28.832116788321166	21.48722627737226	21.98905109489051	27.691605839416056
25	28.51277372262774	21.12226277372263	22.354014598540147	28.01094890510949
26	30.063868613138684	22.445255474452555	21.12226277372263	26.368613138686133
27	26.961678832116785	21.806569343065693	23.631386861313867	27.600364963503647
28	28.01094890510949	22.490875912408757	20.30109489051095	29.1970802919708
29	28.69525547445255	23.03832116788321	21.532846715328464	26.733576642335766
30	27.782846715328468	21.532846715328464	22.354014598540147	28.330291970802918
31	28.193430656934304	21.76094890510949	23.44890510948905	26.596715328467152
32	28.056569343065696	21.624087591240876	21.76094890510949	28.558394160583944
33	25.95802919708029	21.12226277372263	24.224452554744524	28.69525547445255
34	26.779197080291972	20.93978102189781	23.494525547445257	28.786496350364963
35	26.459854014598537	22.62773722627737	23.677007299270073	27.235401459854014
36	27.00729927007299	22.08029197080292	25.18248175182482	25.72992700729927
37	27.600364963503647	22.171532846715326	22.354014598540147	27.874087591240876
38	25.684306569343068	20.574817518248175	24.954379562043798	28.786496350364963
39	25.638686131386862	21.852189781021895	25.136861313868614	27.37226277372263
40	24.361313868613138	22.62773722627737	26.916058394160586	26.094890510948904
41	24.041970802919707	20.483576642335766	25.775547445255476	29.698905109489047
42	22.9014598540146	21.532846715328464	28.284671532846716	27.28102189781022
43	21.943430656934307	22.81021897810219	27.645985401459853	27.600364963503647
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	1.0
15	1.5
16	2.0
17	1.0
18	0.0
19	0.0
20	1.0
21	2.0
22	1.5
23	1.0
24	1.5
25	2.0
26	2.0
27	2.5
28	3.0
29	4.0
30	5.0
31	7.0
32	9.0
33	9.0
34	17.0
35	25.0
36	25.0
37	25.0
38	34.0
39	43.0
40	43.0
41	50.0
42	57.0
43	76.0
44	95.0
45	124.0
46	153.0
47	153.0
48	164.5
49	176.0
50	204.5
51	233.0
52	235.0
53	237.0
54	237.0
55	239.0
56	241.0
57	231.0
58	221.0
59	205.0
60	189.0
61	189.0
62	178.0
63	167.0
64	128.5
65	90.0
66	77.0
67	64.0
68	64.0
69	59.0
70	54.0
71	45.5
72	37.0
73	27.0
74	17.0
75	17.0
76	18.0
77	19.0
78	12.5
79	6.0
80	7.0
81	8.0
82	8.0
83	6.0
84	4.0
85	3.0
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2192.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.78102189781022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.88617886178862	83.3941605839416
2	4.725609756097561	8.485401459854014
3	1.5752032520325203	4.242700729927007
4	0.45731707317073167	1.6423357664233578
5	0.05081300813008131	0.22810218978102187
6	0.05081300813008131	0.2737226277372263
7	0.1524390243902439	0.958029197080292
8	0.05081300813008131	0.36496350364963503
9	0.05081300813008131	0.41058394160583944
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	9	0.41058394160583944	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	8	0.36496350364963503	RNA PCR Primer, Index 39 (95% over 23bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	7	0.3193430656934307	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	7	0.3193430656934307	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	7	0.3193430656934307	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	6	0.2737226277372263	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	5	0.22810218978102187	No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA	4	0.18248175182481752	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	4	0.18248175182481752	No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC	4	0.18248175182481752	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	4	0.18248175182481752	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	4	0.18248175182481752	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	4	0.18248175182481752	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC	4	0.18248175182481752	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	4	0.18248175182481752	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	4	0.18248175182481752	No Hit
CCTTGGTATTCTCTACCTGACCACCTGTGTCGGTTTGGGGTAC	3	0.13686131386861314	No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGG	3	0.13686131386861314	No Hit
CCCCATCAATTAACCTTCCGGCACCGGGCAGGCGTCACACCGT	3	0.13686131386861314	No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	3	0.13686131386861314	RNA PCR Primer, Index 39 (95% over 24bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	3	0.13686131386861314	RNA PCR Primer, Index 39 (95% over 21bp)
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	3	0.13686131386861314	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	3	0.13686131386861314	No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTG	3	0.13686131386861314	No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA	3	0.13686131386861314	No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTT	3	0.13686131386861314	No Hit
GTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT	3	0.13686131386861314	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	3	0.13686131386861314	No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT	3	0.13686131386861314	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	3	0.13686131386861314	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	3	0.13686131386861314	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	3	0.13686131386861314	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	3	0.13686131386861314	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	3	0.13686131386861314	No Hit
CAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGGTTGTAAAGT	3	0.13686131386861314	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	3	0.13686131386861314	No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG	3	0.13686131386861314	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	3	0.13686131386861314	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	3	0.13686131386861314	No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGAT	3	0.13686131386861314	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	3	0.13686131386861314	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	3	0.13686131386861314	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	3	0.13686131386861314	No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT	3	0.13686131386861314	No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	3	0.13686131386861314	No Hit
GGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCA	3	0.13686131386861314	No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA	3	0.13686131386861314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.04562043795620438	0.0
28	0.0	0.0	0.0	0.13686131386861314	0.0
29	0.0	0.0	0.0	0.2737226277372263	0.0
30	0.0	0.0	0.0	0.6843065693430657	0.0
31	0.0	0.0	0.0	1.186131386861314	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE