FastQCFastQC Report
Fri 10 Feb 2017
ERR1632475.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632475.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2802
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT351.2491077801570307RNA PCR Primer, Index 47 (95% over 22bp)
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA180.6423982869379015No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT110.39257673090649536No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA110.39257673090649536No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC110.39257673090649536RNA PCR Primer, Index 47 (95% over 23bp)
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC110.39257673090649536No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC110.39257673090649536No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT100.35688793718772305No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA100.35688793718772305No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA100.35688793718772305No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA90.32119914346895073No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA90.32119914346895073No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT80.28551034975017847No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA80.28551034975017847No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC80.28551034975017847No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG80.28551034975017847No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC80.28551034975017847No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA80.28551034975017847No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA80.28551034975017847No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC70.24982155603140616No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC70.24982155603140616No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT70.24982155603140616No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC60.21413276231263384No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC60.21413276231263384No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA60.21413276231263384No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC60.21413276231263384No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC60.21413276231263384No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT60.21413276231263384No Hit
GTACTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGC50.17844396859386152No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA50.17844396859386152No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC50.17844396859386152No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA50.17844396859386152No Hit
ACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTC50.17844396859386152No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC50.17844396859386152No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT50.17844396859386152No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG50.17844396859386152No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT50.17844396859386152No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG50.17844396859386152No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA50.17844396859386152No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC50.17844396859386152No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC50.17844396859386152No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA50.17844396859386152No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC50.17844396859386152No Hit
CCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCGCCT50.17844396859386152No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA50.17844396859386152No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA50.17844396859386152No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC50.17844396859386152No Hit
CTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAA40.14275517487508924No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA40.14275517487508924No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTTAT40.14275517487508924No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA40.14275517487508924No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG40.14275517487508924No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCCTGTC40.14275517487508924No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA40.14275517487508924No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG40.14275517487508924No Hit
GAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAA40.14275517487508924No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA40.14275517487508924No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG40.14275517487508924No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG40.14275517487508924No Hit
CGTGCGCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCT40.14275517487508924No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT40.14275517487508924No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC40.14275517487508924No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA40.14275517487508924No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGC40.14275517487508924No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG40.14275517487508924No Hit
CTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTCTT40.14275517487508924No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT40.14275517487508924No Hit
CTTTACAACCCGAAGGCCTTCTTCATACACGCGGCATGGCTGC40.14275517487508924No Hit
CAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCT30.10706638115631692No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG30.10706638115631692No Hit
GTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAAC30.10706638115631692No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT30.10706638115631692No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG30.10706638115631692No Hit
AAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAG30.10706638115631692No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC30.10706638115631692No Hit
CACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATC30.10706638115631692No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT30.10706638115631692No Hit
CCCTTATCCTTTGTTGCCAGCGGTCCGGCCGGGAACTCAAAGG30.10706638115631692No Hit
CCCATAGAGAGGCCCGTACTCTGCGTTGATACCCTGTCTCTTA30.10706638115631692No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG30.10706638115631692No Hit
GATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACG30.10706638115631692No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG30.10706638115631692No Hit
TGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTA30.10706638115631692No Hit
CAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAAT30.10706638115631692No Hit
CCGTAGTGCCTCGTCATCACGCCTCAGCCTTGATTTTCCGGAT30.10706638115631692No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG30.10706638115631692No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC30.10706638115631692No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT30.10706638115631692No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTC30.10706638115631692No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG30.10706638115631692No Hit
TTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCAAAGAA30.10706638115631692No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG30.10706638115631692No Hit
AAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACG30.10706638115631692No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGG30.10706638115631692No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT30.10706638115631692No Hit
GTATGAAGAAGGCCTTCGGGTTGTAAAGTACTTTCAGCGGGGA30.10706638115631692No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAG30.10706638115631692No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG30.10706638115631692No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA30.10706638115631692No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACC30.10706638115631692No Hit
GCTCCATGCAGACTGGCGTCCACACTTCAAAGCCTCCCACCTA30.10706638115631692No Hit
GGGAGGAAGGGAGTAAAGTTAATACCTTTGCTCATTGACGTTA30.10706638115631692No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA30.10706638115631692No Hit
GGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCT30.10706638115631692No Hit
CTCCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGA30.10706638115631692No Hit
CATTAAAGGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGC30.10706638115631692No Hit
GAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAAC30.10706638115631692No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT30.10706638115631692No Hit
AACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAA30.10706638115631692No Hit
ATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTA30.10706638115631692No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGAC30.10706638115631692No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG30.10706638115631692No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT30.10706638115631692No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA30.10706638115631692No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT30.10706638115631692No Hit
GTAAAGTACTTTCAGCGGGGAGGAAGGGAGTAAAGTTAATACC30.10706638115631692No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA30.10706638115631692No Hit
GTCTCCACCCGAGACTCAGTGAAATTGAACTCGCTGTGAAGAT30.10706638115631692No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG30.10706638115631692No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC30.10706638115631692No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers