FastQCFastQC Report
Fri 10 Feb 2017
ERR1632473.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632473.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236926
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9290.39210555194448893No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7260.30642479086296986No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6180.260840937676743No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5560.2346724293661312No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5410.2283413386458219No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5310.22412061149894905No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5050.2131467209170796No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4930.20808184834083215No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4710.1987962486177119No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4580.19330930332677715No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT4560.19246515789740257No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4490.18951064889459157No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4440.18740028532115513No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4360.18402370360365686No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4310.18191334003022042No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4300.18149126731553314No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3940.1662966495867908No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3920.16545250415741622No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3570.15067995914336121No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3460.14603715928180105No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3400.14350472299367734No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3400.14350472299367734No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3390.14308265027899006No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3180.13421912327055704No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3050.12873217797962233No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3050.12873217797962233No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3020.12746595983556047No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2930.12366730540337491No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2890.12197901454462576No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2890.12197901454462576No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2890.12197901454462576No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2860.12071279640056388No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2850.12029072368587661No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2840.11986865097118932No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2740.11564792382431645No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2700.11395963296556731No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2670.11269341482150544No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2630.11100512396275629No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2590.10931683310400715No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2570.10847268767463257No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2520.10636232410119616No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2500.10551817867182159No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2490.10509610595713428No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2450.10340781509838515No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2450.10340781509838515No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2420.10214159695432329No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2400.10129745152494872No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2400.10129745152494872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT200.00183898837.013
TTTGCCG200.00183898837.08
CCGGTAT200.00183898837.029
CACCGGT200.00183898837.027
GTTAAGA251.2306694E-436.99999628
TCTATAC251.2306694E-436.9999963
GGTATCA4650.029.8387091
ACCGGTA250.005487649229.59999828
AGATCTC250.005487649229.5999985
GCAACTT250.005487649229.59999813
AACTTAA250.005487649229.59999815
TACGCAT250.005487649229.59999812
TCTACGC250.005487649229.59999810
CTACGCA250.005487649229.59999811
CAACTTA250.005487649229.59999814
GGTCTAT250.005487649229.5999981
CGGTATT250.005487649229.59999830
TAAGACT250.005487649229.59999830
GTTATTG250.005487649229.59999811
ACCCAAG405.9205446E-527.7500021
Produced by FastQC (version 0.11.5)