##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632473.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	236926
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.034263862978314	34.0	33.0	34.0	31.0	34.0
2	33.16590412196213	34.0	33.0	34.0	31.0	34.0
3	33.23603150350743	34.0	34.0	34.0	31.0	34.0
4	36.517659522382516	37.0	37.0	37.0	35.0	37.0
5	36.494120527084405	37.0	37.0	37.0	35.0	37.0
6	36.5457526822721	37.0	37.0	37.0	35.0	37.0
7	36.55267889552012	37.0	37.0	37.0	35.0	37.0
8	36.525320142154094	37.0	37.0	37.0	35.0	37.0
9	38.325316765572374	39.0	39.0	39.0	37.0	39.0
10	38.356343330829034	39.0	39.0	39.0	37.0	39.0
11	38.42170973215266	39.0	39.0	39.0	37.0	39.0
12	38.379764145767034	39.0	39.0	39.0	37.0	39.0
13	38.4211103888978	39.0	39.0	39.0	37.0	39.0
14	39.94107020757536	41.0	40.0	41.0	38.0	41.0
15	39.95089606037328	41.0	40.0	41.0	38.0	41.0
16	39.903066780344915	41.0	40.0	41.0	38.0	41.0
17	39.910951098655275	41.0	40.0	41.0	38.0	41.0
18	39.92986417700041	41.0	40.0	41.0	38.0	41.0
19	39.927766475608415	41.0	40.0	41.0	38.0	41.0
20	39.91140271645999	41.0	40.0	41.0	38.0	41.0
21	39.87114542093312	41.0	40.0	41.0	38.0	41.0
22	39.838485434270616	41.0	40.0	41.0	38.0	41.0
23	39.807230949748025	41.0	40.0	41.0	38.0	41.0
24	39.78559550239315	41.0	40.0	41.0	38.0	41.0
25	39.759156867545144	41.0	40.0	41.0	38.0	41.0
26	39.661877548264016	41.0	40.0	41.0	37.0	41.0
27	39.5563509281379	41.0	40.0	41.0	37.0	41.0
28	39.52499514616378	41.0	40.0	41.0	37.0	41.0
29	39.482526189611946	41.0	39.0	41.0	37.0	41.0
30	39.45319213594118	41.0	39.0	41.0	37.0	41.0
31	39.37916058178503	41.0	39.0	41.0	36.0	41.0
32	39.331533896659714	41.0	39.0	41.0	36.0	41.0
33	39.256582223985546	41.0	39.0	41.0	36.0	41.0
34	39.23148155964309	41.0	39.0	41.0	35.0	41.0
35	39.146020276373214	41.0	39.0	41.0	35.0	41.0
36	39.09119303073533	41.0	39.0	41.0	35.0	41.0
37	39.037095970893866	41.0	39.0	41.0	35.0	41.0
38	38.96451634687624	40.0	39.0	41.0	35.0	41.0
39	38.865291272380404	40.0	39.0	41.0	35.0	41.0
40	38.77923064585567	40.0	38.0	41.0	35.0	41.0
41	38.722618032634664	40.0	38.0	41.0	35.0	41.0
42	38.65466010484286	40.0	38.0	41.0	35.0	41.0
43	37.9053164279142	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	11.0
21	21.0
22	49.0
23	71.0
24	117.0
25	183.0
26	289.0
27	380.0
28	550.0
29	711.0
30	1032.0
31	1426.0
32	1746.0
33	2364.0
34	3599.0
35	5810.0
36	9143.0
37	17829.0
38	47522.0
39	144070.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.721356035217745	16.201683225986173	12.835653326355065	28.241307412441014
2	21.9473590910242	19.46641567409233	31.3059773937854	27.280247841098067
3	22.018267307091666	18.97723339776977	29.03353789790905	29.970961397229516
4	15.716299604095793	16.2903184960705	34.60869638621342	33.38468551362029
5	17.517283877666443	32.37677587094704	32.55953335640664	17.546406894979867
6	32.49580037648886	34.0051324042106	16.002886977368462	17.49618024193208
7	29.188438584199282	27.36761689303833	19.69728944902628	23.746655073736104
8	26.769961929041138	29.8751508909955	20.836041633252577	22.518845546710786
9	28.104555852882335	12.48955370031149	17.612250238471084	41.79364020833509
10	20.072934165097962	22.29008213535028	28.398318462304683	29.238665237247076
11	39.79470383157611	19.2815478250593	18.303605345128858	22.620142998235735
12	22.19469370183095	23.552079552265266	26.698631640258984	27.554595105644804
13	36.684027924330806	17.36998050024058	19.830242354152773	26.11574922127584
14	24.1683057157087	20.394553573689674	22.954846660982753	32.48229404961887
15	30.598161451254825	23.43263297400876	19.35920920456176	26.609996370174656
16	24.826317077906182	23.908308923461334	22.899133062644033	28.36624093598845
17	27.28066991381275	23.38789326625191	21.282594565391726	28.04884225454361
18	26.869571089707335	20.435072554299655	22.891113681064972	29.804242674928037
19	28.436305006626544	22.405730059174594	22.82611448300313	26.33185045119573
20	30.922735368849345	20.045921511357978	21.86336662080143	27.167976498991248
21	27.88296767767151	21.347171690738882	21.578467538387514	29.191393093202095
22	28.878215138904128	22.531507728151407	20.838151996826014	27.75212513611845
23	28.966428336273776	21.155550678270853	21.774731350717104	28.10328963473827
24	28.015920582798003	20.873606104859746	22.678811105577267	28.43166220676498
25	28.81785874070385	21.74349796983024	22.337354279395257	27.101289010070655
26	28.41224686188937	22.52475456471641	21.917391928281404	27.145606645112817
27	27.11479533694065	21.433696597249774	24.626676683859095	26.82483138195048
28	26.4690240834691	23.11903294699611	21.323113546001704	29.088829423533085
29	27.591737504537285	23.612435950465546	21.621941028000304	27.173885516996872
30	27.19034635286967	21.60083739226594	23.634805804343973	27.574010450520415
31	28.554063294024296	22.045702033546338	22.22086221014156	27.179372462287805
32	25.976887298143726	21.546390012071278	22.59946143521606	29.87726125456894
33	26.463115065463477	21.47337143243038	24.11765698994623	27.945856512159917
34	26.46438128360754	21.559474266226587	23.698538784261753	28.277605665904122
35	25.681436397862623	23.30980981403476	24.37554341862016	26.633210369482452
36	26.46100470189004	21.529507103483787	25.073651688712932	26.93583650591324
37	27.038822248296935	21.068181626330585	24.484860251724168	27.40813587364831
38	25.826207339000362	20.30718452174941	25.817765884706617	28.04884225454361
39	25.3800764795759	18.994960451786632	27.30852671298211	28.316436355655348
40	24.922127584140195	20.324489503051584	28.099490980306086	26.653891932502134
41	22.988612478157737	19.552940580603227	29.090517714391833	28.3679292268472
42	21.128538024530865	20.482766771059318	30.51290276288799	27.875792441521824
43	20.37809273781687	21.36447667204106	29.762879548888684	28.494551041253384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	5.5
4	8.0
5	8.0
6	8.5
7	9.0
8	11.0
9	13.0
10	20.0
11	27.0
12	27.0
13	71.0
14	115.0
15	196.5
16	278.0
17	260.5
18	243.0
19	243.0
20	290.0
21	337.0
22	285.5
23	234.0
24	258.0
25	282.0
26	282.0
27	331.5
28	381.0
29	513.5
30	646.0
31	778.0
32	910.0
33	910.0
34	1217.0
35	1524.0
36	1700.5
37	1877.0
38	2594.0
39	3311.0
40	3311.0
41	4371.0
42	5431.0
43	6870.0
44	8309.0
45	12952.0
46	17595.0
47	17595.0
48	20469.5
49	23344.0
50	26378.5
51	29413.0
52	29968.5
53	30524.0
54	30524.0
55	28126.5
56	25729.0
57	24642.0
58	23555.0
59	21756.0
60	19957.0
61	19957.0
62	17806.0
63	15655.0
64	12557.0
65	9459.0
66	8154.5
67	6850.0
68	6850.0
69	5666.5
70	4483.0
71	3703.0
72	2923.0
73	2206.5
74	1490.0
75	1490.0
76	1180.5
77	871.0
78	683.5
79	496.0
80	400.5
81	305.0
82	305.0
83	244.0
84	183.0
85	129.5
86	76.0
87	60.0
88	44.0
89	44.0
90	34.0
91	24.0
92	16.5
93	9.0
94	5.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	236926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.41963142009445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9983148105785	37.31174183794957
2	7.235647967441598	6.428096315986226
3	2.466551882305059	3.2868997647159275
4	1.2918690936398856	2.2953739592998077
5	0.8451082375454434	1.876969821092711
6	0.5893654353690235	1.5707637246501311
7	0.4215450437958995	1.3107412830667
8	0.37159883467603033	1.3205026617956703
9	0.2507293120648801	1.0023573265322232
>10	2.096736348382032	18.99598020998065
>50	0.24573470303371797	7.679858575214728
>100	0.1798058802685741	14.965696211280466
>500	0.006992450899333438	1.9550183084351795
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	929	0.39210555194448893	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	726	0.30642479086296986	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	618	0.260840937676743	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	556	0.2346724293661312	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	541	0.2283413386458219	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	531	0.22412061149894905	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	505	0.2131467209170796	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	493	0.20808184834083215	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	471	0.1987962486177119	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	458	0.19330930332677715	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	456	0.19246515789740257	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	449	0.18951064889459157	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	444	0.18740028532115513	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	436	0.18402370360365686	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	431	0.18191334003022042	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	430	0.18149126731553314	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	394	0.1662966495867908	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	392	0.16545250415741622	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	357	0.15067995914336121	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	346	0.14603715928180105	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	340	0.14350472299367734	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	340	0.14350472299367734	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	339	0.14308265027899006	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	318	0.13421912327055704	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	305	0.12873217797962233	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	305	0.12873217797962233	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	302	0.12746595983556047	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	293	0.12366730540337491	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	289	0.12197901454462576	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	289	0.12197901454462576	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	289	0.12197901454462576	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	286	0.12071279640056388	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	285	0.12029072368587661	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	284	0.11986865097118932	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	274	0.11564792382431645	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	270	0.11395963296556731	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	267	0.11269341482150544	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	263	0.11100512396275629	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	259	0.10931683310400715	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	257	0.10847268767463257	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	252	0.10636232410119616	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	250	0.10551817867182159	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	249	0.10509610595713428	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	245	0.10340781509838515	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	245	0.10340781509838515	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	242	0.10214159695432329	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	240	0.10129745152494872	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	240	0.10129745152494872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.220727146872863E-4	0.0	0.0	0.0	0.0
2	4.220727146872863E-4	0.0	0.0	0.0	0.0
3	4.220727146872863E-4	0.0	0.0	0.0	0.0
4	4.220727146872863E-4	0.0	0.0	0.0	0.0
5	4.220727146872863E-4	0.0	0.0	0.0	0.0
6	4.220727146872863E-4	0.0	0.0	0.0	0.0
7	4.220727146872863E-4	0.0	0.0	0.0	0.0
8	8.441454293745726E-4	0.0	0.0	0.0	0.0
9	8.441454293745726E-4	0.0	0.0	0.0	0.0
10	8.441454293745726E-4	0.0	0.0	0.0	0.0
11	8.441454293745726E-4	0.0	0.0	0.0	0.0
12	8.441454293745726E-4	0.0	0.0	0.0	0.0
13	8.441454293745726E-4	0.0	0.0	4.220727146872863E-4	0.0
14	8.441454293745726E-4	0.0	0.0	4.220727146872863E-4	0.0
15	8.441454293745726E-4	0.0	0.0	4.220727146872863E-4	0.0
16	8.441454293745726E-4	0.0	0.0	8.441454293745726E-4	0.0
17	8.441454293745726E-4	0.0	0.0	0.001266218144061859	0.0
18	8.441454293745726E-4	0.0	0.0	0.0016882908587491452	0.0
19	8.441454293745726E-4	0.0	0.0	0.0016882908587491452	0.0
20	8.441454293745726E-4	0.0	0.0	0.002532436288123718	0.0
21	8.441454293745726E-4	0.0	0.0	0.003798654432185577	0.0
22	8.441454293745726E-4	0.0	0.0	0.007597308864371154	0.0
23	8.441454293745726E-4	0.0	0.0	0.008863527008433012	0.0
24	8.441454293745726E-4	0.0	0.0	0.013506326869993162	0.0
25	8.441454293745726E-4	0.0	0.0	0.014350472299367735	0.0
26	8.441454293745726E-4	0.0	0.0	0.018149126731553313	0.0
27	8.441454293745726E-4	0.0	0.0	0.0400969078952922	0.0
28	8.441454293745726E-4	0.0	0.0	0.16882908587491452	4.220727146872863E-4
29	8.441454293745726E-4	0.0	0.0	0.3562293711960697	4.220727146872863E-4
30	8.441454293745726E-4	0.0	0.0	0.6225572541637473	4.220727146872863E-4
31	8.441454293745726E-4	0.0	0.0	1.3286849058355774	4.220727146872863E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	20	0.001838988	37.0	13
TTTGCCG	20	0.001838988	37.0	8
CCGGTAT	20	0.001838988	37.0	29
CACCGGT	20	0.001838988	37.0	27
GTTAAGA	25	1.2306694E-4	36.999996	28
TCTATAC	25	1.2306694E-4	36.999996	3
GGTATCA	465	0.0	29.838709	1
ACCGGTA	25	0.0054876492	29.599998	28
AGATCTC	25	0.0054876492	29.599998	5
GCAACTT	25	0.0054876492	29.599998	13
AACTTAA	25	0.0054876492	29.599998	15
TACGCAT	25	0.0054876492	29.599998	12
TCTACGC	25	0.0054876492	29.599998	10
CTACGCA	25	0.0054876492	29.599998	11
CAACTTA	25	0.0054876492	29.599998	14
GGTCTAT	25	0.0054876492	29.599998	1
CGGTATT	25	0.0054876492	29.599998	30
TAAGACT	25	0.0054876492	29.599998	30
GTTATTG	25	0.0054876492	29.599998	11
ACCCAAG	40	5.9205446E-5	27.750002	1
>>END_MODULE