FastQCFastQC Report
Fri 10 Feb 2017
ERR1632472.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632472.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10130
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA580.5725567620927937No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA490.48371174728529126No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA420.4146100691016782No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT390.38499506416584406No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC350.3455083909180652No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT350.3455083909180652RNA PCR Primer, Index 46 (95% over 21bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA300.29615004935834155No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC290.28627838104639686No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA290.28627838104639686No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT280.2764067127344521No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT270.2665350444225074No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC270.2665350444225074No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA270.2665350444225074No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA270.2665350444225074No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA250.24679170779861795No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA250.24679170779861795No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC250.24679170779861795No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT240.23692003948667323No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC230.22704837117472854No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA220.21717670286278382No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC220.21717670286278382No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT220.21717670286278382No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA210.2073050345508391No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA210.2073050345508391No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG210.2073050345508391No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC200.19743336623889435No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC200.19743336623889435No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA200.19743336623889435No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT190.18756169792694966No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA190.18756169792694966No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA180.17769002961500494No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC180.17769002961500494No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG180.17769002961500494No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA180.17769002961500494No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT170.16781836130306022No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC170.16781836130306022No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT160.1579466929911155No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC160.1579466929911155No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG160.1579466929911155No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC160.1579466929911155No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG160.1579466929911155No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA160.1579466929911155No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT150.14807502467917077No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA150.14807502467917077No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC150.14807502467917077No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGCTGTC150.14807502467917077No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG150.14807502467917077No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGCTGTCT140.13820335636722605No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC140.13820335636722605No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC140.13820335636722605No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC140.13820335636722605No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT140.13820335636722605No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC140.13820335636722605No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA140.13820335636722605No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA140.13820335636722605No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA140.13820335636722605No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG140.13820335636722605No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG140.13820335636722605No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA140.13820335636722605No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATT140.13820335636722605No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA130.12833168805528133No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG130.12833168805528133No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG130.12833168805528133No Hit
GCTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTA130.12833168805528133No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT130.12833168805528133No Hit
TATCAACGCAGAGTACGGGGATTTCACATCTGACTTAACAAAC130.12833168805528133No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG130.12833168805528133No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA130.12833168805528133No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG130.12833168805528133No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCTGTCT120.11846001974333661No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA120.11846001974333661No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC120.11846001974333661No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG120.11846001974333661No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC120.11846001974333661No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT120.11846001974333661No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC120.11846001974333661No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG120.11846001974333661No Hit
GGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCAGATGTGAAA120.11846001974333661No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT120.11846001974333661No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT120.11846001974333661No Hit
CATTGACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCC120.11846001974333661No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA120.11846001974333661No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG120.11846001974333661No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG110.10858835143139191No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGACTG110.10858835143139191No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT110.10858835143139191No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTG110.10858835143139191No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA110.10858835143139191No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC110.10858835143139191No Hit
CCCTTCCTCCCCGCTGAAAGTACTTTACAACCCGAAGGCCTTC110.10858835143139191No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA110.10858835143139191No Hit
CAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCCGATTAAC110.10858835143139191No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG110.10858835143139191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT358.273132E-426.42857237
Produced by FastQC (version 0.11.5)