FastQCFastQC Report
Fri 10 Feb 2017
ERR1632471.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632471.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36349
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1800.49519931772538445No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1380.3796528102561281RNA PCR Primer, Index 27 (95% over 23bp)
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1250.3438884150870725No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA940.25860408814547853No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC900.24759965886269222No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT820.2255908002971196No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA790.21733747833502984No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG690.18982640512806406No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC680.18707529780736745No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC640.17607086852458115No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA630.17331976120388456No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT620.17056865388318798No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA620.17056865388318798No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT600.16506643924179482No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA570.15681311727970507No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA570.15681311727970507No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC570.15681311727970507No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA570.15681311727970507No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT560.1540620099590085No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC540.14855979531761535No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT540.14855979531761535No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA540.14855979531761535No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT520.14305758067622218No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC510.1403064733555256No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC450.12379982943134611No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA440.12104872211064954No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC430.11829761478995295No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC430.11829761478995295No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA420.11554650746925638No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC410.1127954001485598No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA410.1127954001485598No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA400.11004429282786322No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG400.11004429282786322No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA400.11004429282786322No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT390.10729318550716663No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG390.10729318550716663No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC380.10454207818647006RNA PCR Primer, Index 27 (95% over 21bp)
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA380.10454207818647006No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC370.10179097086577348No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC370.10179097086577348No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA370.10179097086577348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGAG303.5318595E-430.83333233
TTCTGCG303.5318595E-430.83333218
TCTGCGG303.5318595E-430.83333219
CTGCGGG303.5318595E-430.83333220
TTCTTCT303.5318595E-430.83333215
TCTTCTG303.5318595E-430.83333216
TGCGGGT303.5318595E-430.83333221
AATGAGC303.5318595E-430.83333234
TTCGCCT250.00542737429.625
CCTCATT250.00542737429.629
CTCATTA250.00542737429.630
GGTATCA453.8695725E-628.7777771
AACGTCA358.704335E-426.42857228
TAACGTC358.704335E-426.42857227
TCAATGA358.704335E-426.42857232
GAATATG358.704335E-426.42857215
GCTTCTT358.704335E-426.42857213
GTAACGT358.704335E-426.42857226
GGTAACG358.704335E-426.42857225
ATCCTCC400.001895586723.12530
Produced by FastQC (version 0.11.5)