FastQCFastQC Report
Fri 10 Feb 2017
ERR1632470.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632470.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458008
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA62230.17995909783898706No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA53050.15341202218155653No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA41430.1198088610552665No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA36110.10442428126250719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.030.1442741
GTATCAA53050.019.9820942
GCGGTAA27200.019.92830823
ATACGGC5700.019.14912229
TATTAGA4450.019.1235942
CTTATAC47950.018.71219837
TAATACG26900.018.527
CGGTAAT29600.018.2524
ATACACA9900.018.1262637
GGTAATA30650.017.9265925
CGCGGTA30600.017.89542422
TATACAC15700.017.55732537
GTAATAC31200.017.19551326
AACTCCG32450.017.1602465
CGTGCCA32450.017.10323510
TATACTG1301.3958925E-817.0769235
AGCCGCG33400.016.94910219
AATACGG29600.016.87528
TAAACCG2200.016.8181825
TAATACT4650.016.7096774
Produced by FastQC (version 0.11.5)