##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632470.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3458008
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.02559681758978	34.0	33.0	34.0	31.0	34.0
2	33.1645742288624	34.0	33.0	34.0	31.0	34.0
3	33.24760758216869	34.0	34.0	34.0	31.0	34.0
4	36.53654560660357	37.0	37.0	37.0	35.0	37.0
5	36.50209224501505	37.0	37.0	37.0	35.0	37.0
6	36.54086254282812	37.0	37.0	37.0	35.0	37.0
7	36.53897937772267	37.0	37.0	37.0	35.0	37.0
8	36.51042189607427	37.0	37.0	37.0	35.0	37.0
9	38.30669853858059	39.0	39.0	39.0	37.0	39.0
10	38.33415249473107	39.0	39.0	39.0	37.0	39.0
11	38.40656268001693	39.0	39.0	39.0	37.0	39.0
12	38.36507318664387	39.0	39.0	39.0	37.0	39.0
13	38.39821018343509	39.0	39.0	39.0	37.0	39.0
14	39.953028448748526	41.0	40.0	41.0	38.0	41.0
15	39.94729798195956	41.0	40.0	41.0	38.0	41.0
16	39.915153464075274	41.0	40.0	41.0	38.0	41.0
17	39.910785342312685	41.0	40.0	41.0	38.0	41.0
18	39.9063955317628	41.0	40.0	41.0	38.0	41.0
19	39.915318877226426	41.0	40.0	41.0	38.0	41.0
20	39.88037332475807	41.0	40.0	41.0	38.0	41.0
21	39.85242920201457	41.0	40.0	41.0	38.0	41.0
22	39.82103135678113	41.0	40.0	41.0	38.0	41.0
23	39.78292126565352	41.0	40.0	41.0	38.0	41.0
24	39.76330476968243	41.0	40.0	41.0	38.0	41.0
25	39.71891620840669	41.0	40.0	41.0	38.0	41.0
26	39.63373479760602	41.0	40.0	41.0	37.0	41.0
27	39.5576785825828	41.0	40.0	41.0	37.0	41.0
28	39.51316711817902	41.0	40.0	41.0	37.0	41.0
29	39.46633379679862	41.0	40.0	41.0	37.0	41.0
30	39.41980440762428	41.0	39.0	41.0	37.0	41.0
31	39.363355145505736	41.0	39.0	41.0	36.0	41.0
32	39.30790472433841	41.0	39.0	41.0	36.0	41.0
33	39.24431724854309	41.0	39.0	41.0	36.0	41.0
34	39.201544068145594	41.0	39.0	41.0	35.0	41.0
35	39.120938123914115	41.0	39.0	41.0	35.0	41.0
36	39.07353250773278	41.0	39.0	41.0	35.0	41.0
37	39.01949532794603	41.0	39.0	41.0	35.0	41.0
38	38.94332893388332	40.0	39.0	41.0	35.0	41.0
39	38.860833751685945	40.0	39.0	41.0	35.0	41.0
40	38.78451553611212	40.0	38.0	41.0	35.0	41.0
41	38.72501162518999	40.0	38.0	41.0	35.0	41.0
42	38.65340161156365	40.0	38.0	41.0	35.0	41.0
43	37.918619332286106	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	2.0
15	5.0
16	10.0
17	16.0
18	47.0
19	94.0
20	191.0
21	340.0
22	684.0
23	1313.0
24	2025.0
25	2981.0
26	4263.0
27	5889.0
28	8212.0
29	11150.0
30	15233.0
31	20253.0
32	26442.0
33	35965.0
34	53041.0
35	83937.0
36	137672.0
37	262832.0
38	687097.0
39	2098311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62890427089816	16.027001672639276	12.0679593569477	28.276134699514866
2	21.05171532281013	18.8436232651862	32.2580514562141	27.846609955789575
3	21.378955745620022	19.426213010496216	28.152074836148444	31.04275640773532
4	15.94629046549343	15.137240862369318	32.64940393428818	36.267064737849076
5	17.615864393604642	32.583585694422915	32.547235286905064	17.25331462506738
6	35.96978375989876	32.64301297162991	14.703985647228116	16.683217621243212
7	31.534079736079267	27.042707824851764	19.169880462971744	22.253331976097222
8	27.935447228577843	30.88309801481084	19.212245894167975	21.96920886244335
9	28.0726649562407	13.041728070033384	17.189115814653984	41.69649115907193
10	19.560683491767513	23.388465266708465	29.548717064853523	27.502134176670502
11	39.546264785969264	19.355709992573757	18.785208131386625	22.312817090070354
12	23.53580442844551	22.972416489493373	26.429956206000682	27.061822876060436
13	34.623199252286284	17.46904576276284	20.794457387027446	27.11329759792343
14	24.542106322483928	18.8829233477771	22.51197799426722	34.062992335471755
15	29.203865346754547	23.782622827940248	19.80544868606435	27.208063139240856
16	26.662431087493143	23.365070294805566	21.850556736710846	28.12194188099044
17	27.07521208742143	23.15529634402234	21.947201972927765	27.82228959562846
18	26.89429868294116	21.712731723003532	23.100004395594226	28.29296519846108
19	28.60239189730041	22.084622129272113	22.741532119069706	26.571453854357767
20	29.378011849596646	21.273808504780785	22.313684641562425	27.034495004060137
21	27.75855347934418	22.187774001679582	21.77617865545713	28.277493863519116
22	28.312745372480343	22.629791486890717	21.168169651429377	27.889293489199563
23	28.24261829353778	22.110590837268163	22.0328871419615	27.613903727232557
24	28.081918838822812	21.789770295499604	21.903072520364326	28.225238345313258
25	28.448025568477576	21.879388364630735	22.319092379196345	27.353493687695345
26	28.003029489810316	22.375020532051977	22.17048080860426	27.451469169533443
27	27.611445664671685	21.86764750110468	22.958969441366243	27.561937392857388
28	27.011273542455655	22.64792331307504	22.102233424561195	28.238569719908106
29	27.537530277547074	23.12704308376383	21.997866980064824	27.337559658624272
30	27.362024610700725	22.25489356878295	23.09931035440057	27.283771466115752
31	27.810201711505584	22.105327691549586	22.336674756102358	27.74779584084247
32	25.901964367925117	22.092198745636217	23.00489183367997	29.0009450527587
33	26.429117572891673	21.79086919405623	23.763478858348506	28.01653437470359
34	26.768474798207524	21.483090843051837	23.8375966741546	27.91083768458604
35	26.350980101838978	22.280052562053065	24.093582201082242	27.275385135025715
36	26.34158162734152	21.214670411404484	24.835888176082875	27.60785978517112
37	26.41555485123227	20.50050202313008	24.85052087791584	28.233422247721812
38	25.390137905985178	20.139427092129342	26.26963269026561	28.20080231161987
39	24.860555556840815	19.537519866929166	27.366998572588614	28.2349260036414
40	24.635397026264833	19.99197225686002	28.27810114956356	27.094529567311586
41	23.221027828738393	19.734801076226542	29.065404128619715	27.978766966415346
42	21.97048127129839	20.37756997670335	29.740850801964598	27.911097950033657
43	21.316202854360082	20.568402386576317	29.539058324908446	28.576336434155152
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	25.0
2	30.0
3	66.0
4	102.0
5	102.0
6	143.0
7	184.0
8	178.5
9	173.0
10	286.5
11	400.0
12	400.0
13	823.0
14	1246.0
15	2219.0
16	3192.0
17	3190.0
18	3188.0
19	3188.0
20	3966.5
21	4745.0
22	4803.5
23	4862.0
24	6051.0
25	7240.0
26	7240.0
27	8970.0
28	10700.0
29	14442.0
30	18184.0
31	23001.5
32	27819.0
33	27819.0
34	36272.0
35	44725.0
36	52396.0
37	60067.0
38	75616.5
39	91166.0
40	91166.0
41	108064.5
42	124963.0
43	142178.0
44	159393.0
45	192229.5
46	225066.0
47	225066.0
48	251864.0
49	278662.0
50	306029.5
51	333397.0
52	346983.0
53	360569.0
54	360569.0
55	342671.0
56	324773.0
57	310590.0
58	296407.0
59	281834.5
60	267262.0
61	267262.0
62	249797.0
63	232332.0
64	201465.5
65	170599.0
66	151035.0
67	131471.0
68	131471.0
69	114062.0
70	96653.0
71	82904.5
72	69156.0
73	56440.0
74	43724.0
75	43724.0
76	35769.0
77	27814.0
78	22551.0
79	17288.0
80	13575.0
81	9862.0
82	9862.0
83	7650.5
84	5439.0
85	4177.0
86	2915.0
87	2140.0
88	1365.0
89	1365.0
90	978.5
91	592.0
92	385.5
93	179.0
94	115.5
95	52.0
96	52.0
97	39.0
98	26.0
99	16.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3458008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.81660454328391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34962124534202	39.3767927337773
2	9.699636202821956	9.276073370481148
3	2.8781541516440687	4.128706766513257
4	1.332782226179806	2.5491648260622934
5	0.7602696564936522	1.817675675540763
6	0.5169192387919687	1.4830393693278543
7	0.3689131844663698	1.2348123096702366
8	0.2720252785626656	1.0405860136646101
9	0.21534456267646535	0.9267341214642264
>10	1.31604181727037	12.102035389436475
>50	0.1493341164043887	5.008712469051381
>100	0.12172708130186348	11.322731714897532
>500	0.012861269607328273	4.3278505073951425
>1k	0.006248635704953288	5.070673633957185
>5k	1.213327321350153E-4	0.3344110987606246
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	6223	0.17995909783898706	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	5305	0.15341202218155653	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	4143	0.1198088610552665	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	3611	0.10442428126250719	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.891838306909643E-5
7	0.0	0.0	0.0	8.675514920728928E-5	2.891838306909643E-5
8	0.0	0.0	0.0	8.675514920728928E-5	2.891838306909643E-5
9	0.0	0.0	0.0	8.675514920728928E-5	2.891838306909643E-5
10	2.891838306909643E-5	0.0	0.0	1.1567353227638572E-4	2.891838306909643E-5
11	2.891838306909643E-5	0.0	0.0	1.4459191534548215E-4	5.783676613819286E-5
12	2.891838306909643E-5	0.0	0.0	2.0242868148367499E-4	8.675514920728928E-5
13	2.891838306909643E-5	0.0	0.0	2.891838306909643E-4	8.675514920728928E-5
14	2.891838306909643E-5	0.0	0.0	3.7593897989825355E-4	8.675514920728928E-5
15	2.891838306909643E-5	0.0	0.0	4.6269412910554286E-4	1.1567353227638572E-4
16	5.783676613819286E-5	0.0	0.0	8.097147259346999E-4	1.4459191534548215E-4
17	8.675514920728928E-5	0.0	0.0	0.0010988985566256642	1.4459191534548215E-4
18	8.675514920728928E-5	5.783676613819286E-5	0.0	0.0013302456211784356	1.4459191534548215E-4
19	1.1567353227638572E-4	5.783676613819286E-5	0.0	0.0016772662180075928	1.4459191534548215E-4
20	1.4459191534548215E-4	5.783676613819286E-5	0.0	0.002573736093149582	1.4459191534548215E-4
21	1.7351029841457857E-4	5.783676613819286E-5	0.0	0.003701553032844343	1.4459191534548215E-4
22	1.7351029841457857E-4	5.783676613819286E-5	0.0	0.0050896354201609715	1.4459191534548215E-4
23	1.7351029841457857E-4	5.783676613819286E-5	0.0	0.00607286044451025	1.7351029841457857E-4
24	2.0242868148367499E-4	5.783676613819286E-5	0.0	0.00801039211013971	1.7351029841457857E-4
25	2.0242868148367499E-4	5.783676613819286E-5	0.0	0.01003467892497646	1.7351029841457857E-4
26	2.0242868148367499E-4	5.783676613819286E-5	0.0	0.013909742256235381	1.7351029841457857E-4
27	2.0242868148367499E-4	5.783676613819286E-5	0.0	0.047628576914801816	1.7351029841457857E-4
28	2.0242868148367499E-4	5.783676613819286E-5	0.0	0.17865777060087773	1.7351029841457857E-4
29	2.0242868148367499E-4	5.783676613819286E-5	0.0	0.3725844474622384	1.7351029841457857E-4
30	2.0242868148367499E-4	5.783676613819286E-5	0.0	0.6200679697675656	1.7351029841457857E-4
31	2.0242868148367499E-4	5.783676613819286E-5	0.0	1.3202109422534591	1.7351029841457857E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3535	0.0	30.144274	1
GTATCAA	5305	0.0	19.982094	2
GCGGTAA	2720	0.0	19.928308	23
ATACGGC	570	0.0	19.149122	29
TATTAGA	445	0.0	19.123594	2
CTTATAC	4795	0.0	18.712198	37
TAATACG	2690	0.0	18.5	27
CGGTAAT	2960	0.0	18.25	24
ATACACA	990	0.0	18.12626	37
GGTAATA	3065	0.0	17.92659	25
CGCGGTA	3060	0.0	17.895424	22
TATACAC	1570	0.0	17.557325	37
GTAATAC	3120	0.0	17.195513	26
AACTCCG	3245	0.0	17.160246	5
CGTGCCA	3245	0.0	17.103235	10
TATACTG	130	1.3958925E-8	17.076923	5
AGCCGCG	3340	0.0	16.949102	19
AATACGG	2960	0.0	16.875	28
TAAACCG	220	0.0	16.818182	5
TAATACT	465	0.0	16.709677	4
>>END_MODULE