ERR1632469.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632469.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30125
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	94	0.3120331950207469	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	83	0.27551867219917014	RNA PCR Primer, Index 26 (100% over 22bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	68	0.22572614107883815	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	63	0.2091286307053942	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	59	0.195850622406639	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	55	0.18257261410788383	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	52	0.17261410788381742	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	51	0.1692946058091286	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	51	0.1692946058091286	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	47	0.15601659751037344	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	47	0.15601659751037344	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	46	0.15269709543568466	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	44	0.14605809128630703	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	44	0.14605809128630703	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	42	0.13941908713692946	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	42	0.13941908713692946	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	42	0.13941908713692946	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	42	0.13941908713692946	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	41	0.13609958506224065	No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	41	0.13609958506224065	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	41	0.13609958506224065	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	40	0.13278008298755187	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	39	0.12946058091286308	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	38	0.12614107883817427	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	38	0.12614107883817427	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	38	0.12614107883817427	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	38	0.12614107883817427	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	37	0.12282157676348547	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	37	0.12282157676348547	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	37	0.12282157676348547	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	37	0.12282157676348547	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	35	0.11618257261410789	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	34	0.11286307053941907	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	32	0.1062240663900415	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	32	0.1062240663900415	No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT	31	0.10290456431535269	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	50	6.841219E-9	33.3	1
TATGCCG	25	0.0054127797	29.599998	33
CCGTCTT	25	0.0054127797	29.599998	37
ATGCCGT	25	0.0054127797	29.599998	34
TCTCCGA	25	0.0054127797	29.599998	1
GCCGTCT	25	0.0054127797	29.599998	36
TCTTATA	70	6.0645107E-9	26.42857	37
CAGCCGC	40	0.0018880758	23.125	18
GTATCAA	80	6.5092354E-7	20.8125	2
CTCTTAT	105	4.426238E-7	17.619047	36
TCTCTTA	170	2.1398591E-8	14.147059	37
AACGCAG	120	3.0958297E-5	13.874999	7
TATCAAC	120	3.0958297E-5	13.874999	3
ATCAACG	120	3.0958297E-5	13.874999	4
CAACGCA	120	3.0958297E-5	13.874999	6
TCAACGC	130	6.5690154E-5	12.807693	5
AGAGTAC	130	6.5690154E-5	12.807693	12
ACGCAGA	135	9.353156E-5	12.333334	8
CAGAGTA	135	9.353156E-5	12.333334	11
AGTACGG	135	9.353156E-5	12.333334	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content