##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632469.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30125
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.03877178423237	34.0	33.0	34.0	31.0	34.0
2	33.175435684647304	34.0	33.0	34.0	31.0	34.0
3	33.250091286307054	34.0	34.0	34.0	31.0	34.0
4	36.5402489626556	37.0	37.0	37.0	35.0	37.0
5	36.50698755186722	37.0	37.0	37.0	35.0	37.0
6	36.54844813278008	37.0	37.0	37.0	35.0	37.0
7	36.55528630705394	37.0	37.0	37.0	35.0	37.0
8	36.50655601659751	37.0	37.0	37.0	35.0	37.0
9	38.30323651452282	39.0	39.0	39.0	37.0	39.0
10	38.34277178423236	39.0	39.0	39.0	37.0	39.0
11	38.41052282157676	39.0	39.0	39.0	37.0	39.0
12	38.38336929460581	39.0	39.0	39.0	37.0	39.0
13	38.40570954356846	39.0	39.0	39.0	37.0	39.0
14	39.936132780082986	41.0	40.0	41.0	38.0	41.0
15	39.95050622406639	41.0	40.0	41.0	38.0	41.0
16	39.90891286307054	41.0	40.0	41.0	38.0	41.0
17	39.926771784232365	41.0	40.0	41.0	38.0	41.0
18	39.912896265560164	41.0	40.0	41.0	38.0	41.0
19	39.913626556016595	41.0	40.0	41.0	38.0	41.0
20	39.88813278008299	41.0	40.0	41.0	38.0	41.0
21	39.84288796680498	41.0	40.0	41.0	38.0	41.0
22	39.80039834024896	41.0	40.0	41.0	38.0	41.0
23	39.78190871369294	41.0	40.0	41.0	38.0	41.0
24	39.76126141078838	41.0	40.0	41.0	38.0	41.0
25	39.721593360995854	41.0	40.0	41.0	38.0	41.0
26	39.60305394190871	41.0	40.0	41.0	37.0	41.0
27	39.504497925311206	41.0	40.0	41.0	37.0	41.0
28	39.49546887966805	41.0	40.0	41.0	37.0	41.0
29	39.4388713692946	41.0	39.0	41.0	36.0	41.0
30	39.40680497925311	41.0	39.0	41.0	36.0	41.0
31	39.335834024896265	41.0	39.0	41.0	36.0	41.0
32	39.27196680497925	41.0	39.0	41.0	36.0	41.0
33	39.216896265560166	41.0	39.0	41.0	35.0	41.0
34	39.135203319502075	41.0	39.0	41.0	35.0	41.0
35	39.04873029045643	41.0	39.0	41.0	35.0	41.0
36	38.99120331950208	40.0	39.0	41.0	35.0	41.0
37	38.9349377593361	40.0	39.0	41.0	35.0	41.0
38	38.84265560165975	40.0	39.0	41.0	35.0	41.0
39	38.75319502074689	40.0	38.0	41.0	35.0	41.0
40	38.681427385892114	40.0	38.0	41.0	35.0	41.0
41	38.63455601659751	40.0	38.0	41.0	35.0	41.0
42	38.56265560165975	40.0	38.0	41.0	35.0	41.0
43	37.82771784232365	40.0	37.0	41.0	34.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	4.0
22	9.0
23	12.0
24	18.0
25	29.0
26	41.0
27	51.0
28	62.0
29	103.0
30	147.0
31	187.0
32	222.0
33	321.0
34	472.0
35	789.0
36	1195.0
37	2354.0
38	6051.0
39	18056.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.26058091286307	16.972614107883818	13.181742738589211	27.5850622406639
2	21.5103734439834	19.9701244813278	31.598340248962653	26.92116182572614
3	21.483817427385894	19.531950207468878	28.81659751037344	30.167634854771784
4	16.24564315352697	16.52448132780083	33.49045643153527	33.73941908713693
5	17.84564315352697	32.29211618257261	32.21908713692946	17.643153526970952
6	34.34688796680498	33.04896265560166	15.236514522821576	17.367634854771786
7	30.087966804979256	27.21659751037344	19.535269709543567	23.160165975103734
8	27.067219917012448	30.011618257261414	20.766804979253113	22.15435684647303
9	27.86721991701245	12.726970954356847	17.447302904564317	41.95850622406639
10	20.484647302904566	22.95435684647303	28.600829875518674	27.960165975103735
11	39.48879668049792	19.150207468879668	18.164315352697095	23.19668049792531
12	23.09045643153527	23.316182572614107	25.872199170124482	27.721161825726142
13	35.711203319502076	17.171784232365145	20.8597510373444	26.25726141078838
14	24.58755186721992	19.91701244813278	22.907883817427386	32.587551867219915
15	30.376763485477177	23.38921161825726	19.296265560165978	26.93775933609959
16	25.510373443983404	23.20331950207469	22.463070539419085	28.823236514522822
17	26.904564315352697	23.478838174273857	21.470539419087135	28.146058091286307
18	26.808298755186723	20.63734439834025	23.1701244813278	29.38423236514523
19	29.042323651452286	21.998340248962656	22.277178423236517	26.68215767634855
20	30.170954356846476	20.312033195020746	22.33692946058091	27.180082987551867
21	27.966804979253112	21.470539419087135	21.400829875518674	29.16182572614108
22	28.494605809128633	22.619087136929462	20.60746887966805	28.278838174273858
23	28.50124481327801	21.646473029045644	21.71286307053942	28.13941908713693
24	27.790871369294607	21.09875518672199	22.50954356846473	28.600829875518674
25	29.42738589211618	21.480497925311205	22.087966804979253	27.00414937759336
26	28.25228215767635	22.63900414937759	21.885477178423237	27.22323651452282
27	27.55850622406639	21.699585062240665	23.800829875518673	26.941078838174278
28	26.400000000000002	23.2896265560166	21.2149377593361	29.095435684647303
29	26.828215767634855	23.59170124481328	21.87551867219917	27.704564315352698
30	27.219917012448132	21.82240663900415	23.402489626556015	27.555186721991703
31	28.33526970954357	21.669709543568462	22.054771784232365	27.940248962655602
32	25.344398340248965	21.95850622406639	22.801659751037345	29.895435684647303
33	25.995020746887963	21.204979253112032	24.458091286307056	28.34190871369295
34	26.187551867219916	21.37427385892116	23.843983402489627	28.594190871369296
35	26.217427385892115	22.40663900414938	24.873029045643154	26.502904564315354
36	26.48630705394191	21.76597510373444	24.783402489626553	26.964315352697092
37	26.874688796680495	20.83319502074689	24.3551867219917	27.936929460580913
38	25.138589211618257	19.923651452282158	26.625726141078836	28.312033195020746
39	24.959336099585062	18.712033195020748	27.78091286307054	28.54771784232365
40	24.458091286307056	20.089626556016597	29.02240663900415	26.429875518672198
41	22.72863070539419	19.691286307053943	29.457261410788384	28.122821576763485
42	21.311203319502077	20.302074688796683	30.960995850622407	27.425726141078837
43	19.930290456431536	21.341078838174273	30.240663900414937	28.487966804979255
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	1.5
9	3.0
10	2.5
11	2.0
12	2.0
13	10.5
14	19.0
15	24.5
16	30.0
17	36.5
18	43.0
19	43.0
20	41.0
21	39.0
22	35.5
23	32.0
24	36.0
25	40.0
26	40.0
27	60.0
28	80.0
29	96.0
30	112.0
31	136.0
32	160.0
33	160.0
34	211.5
35	263.0
36	290.5
37	318.0
38	430.0
39	542.0
40	542.0
41	641.0
42	740.0
43	951.5
44	1163.0
45	1655.0
46	2147.0
47	2147.0
48	2461.0
49	2775.0
50	3108.5
51	3442.0
52	3574.0
53	3706.0
54	3706.0
55	3448.5
56	3191.0
57	3089.5
58	2988.0
59	2757.5
60	2527.0
61	2527.0
62	2234.5
63	1942.0
64	1569.5
65	1197.0
66	1055.5
67	914.0
68	914.0
69	769.0
70	624.0
71	536.5
72	449.0
73	352.5
74	256.0
75	256.0
76	216.0
77	176.0
78	129.0
79	82.0
80	73.5
81	65.0
82	65.0
83	45.5
84	26.0
85	21.0
86	16.0
87	11.5
88	7.0
89	7.0
90	7.0
91	7.0
92	4.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	30125.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.57510373443984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.71709976912119	58.599170124481326
2	6.607063909220416	8.929460580912863
3	2.3186127621948223	4.700414937759336
4	1.2477280542319595	3.3726141078838174
5	0.677899494031537	2.2904564315352696
6	0.5207054084590068	2.1112033195020747
7	0.3045635407967775	1.4406639004149377
8	0.28000196492606966	1.5136929460580912
9	0.1964926069656629	1.1950207468879668
>10	1.0856216534852876	13.935269709543569
>50	0.044210836567274155	1.912033195020747
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	94	0.3120331950207469	No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	83	0.27551867219917014	RNA PCR Primer, Index 26 (100% over 22bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	68	0.22572614107883815	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	63	0.2091286307053942	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	59	0.195850622406639	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	55	0.18257261410788383	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	52	0.17261410788381742	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	51	0.1692946058091286	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	51	0.1692946058091286	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	47	0.15601659751037344	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	47	0.15601659751037344	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	46	0.15269709543568466	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	44	0.14605809128630703	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	44	0.14605809128630703	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	42	0.13941908713692946	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	42	0.13941908713692946	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	42	0.13941908713692946	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	42	0.13941908713692946	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	41	0.13609958506224065	No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC	41	0.13609958506224065	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	41	0.13609958506224065	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	40	0.13278008298755187	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	39	0.12946058091286308	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	38	0.12614107883817427	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	38	0.12614107883817427	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	38	0.12614107883817427	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	38	0.12614107883817427	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	37	0.12282157676348547	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	37	0.12282157676348547	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	37	0.12282157676348547	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	37	0.12282157676348547	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	35	0.11618257261410789	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	34	0.11286307053941907	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	32	0.1062240663900415	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	32	0.1062240663900415	No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGAT	31	0.10290456431535269	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0033195020746887966	0.0
21	0.0	0.0	0.0	0.0033195020746887966	0.0
22	0.0	0.0	0.0	0.006639004149377593	0.0
23	0.0	0.0	0.0	0.006639004149377593	0.0
24	0.0	0.0	0.0	0.016597510373443983	0.0
25	0.0	0.0	0.0	0.01991701244813278	0.0
26	0.0	0.0	0.0	0.01991701244813278	0.0
27	0.0	0.0	0.0	0.04979253112033195	0.0
28	0.0	0.0	0.0	0.22240663900414936	0.0
29	0.0	0.0	0.0	0.4215767634854772	0.0
30	0.0	0.0	0.0	0.7203319502074689	0.0
31	0.0	0.0	0.0	1.404149377593361	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	50	6.841219E-9	33.3	1
TATGCCG	25	0.0054127797	29.599998	33
CCGTCTT	25	0.0054127797	29.599998	37
ATGCCGT	25	0.0054127797	29.599998	34
TCTCCGA	25	0.0054127797	29.599998	1
GCCGTCT	25	0.0054127797	29.599998	36
TCTTATA	70	6.0645107E-9	26.42857	37
CAGCCGC	40	0.0018880758	23.125	18
GTATCAA	80	6.5092354E-7	20.8125	2
CTCTTAT	105	4.426238E-7	17.619047	36
TCTCTTA	170	2.1398591E-8	14.147059	37
AACGCAG	120	3.0958297E-5	13.874999	7
TATCAAC	120	3.0958297E-5	13.874999	3
ATCAACG	120	3.0958297E-5	13.874999	4
CAACGCA	120	3.0958297E-5	13.874999	6
TCAACGC	130	6.5690154E-5	12.807693	5
AGAGTAC	130	6.5690154E-5	12.807693	12
ACGCAGA	135	9.353156E-5	12.333334	8
CAGAGTA	135	9.353156E-5	12.333334	11
AGTACGG	135	9.353156E-5	12.333334	14
>>END_MODULE